Pistil Factors Controlling Pollination

Sánchez, Ana

doi:10.1105/tpc.017806

Cited by 108 publications

(86 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The pollen coat becomes liquid at pollination and is necessary for pollen germination (Edlund et al, 2004). In wet stigma-type plants (e.g., tobacco [Nicotiana tabacum]), the function of the pollen coat is carried by the stigmatic exudate (Sanchez et al, 2004). This exudate is secreted by the secretory zone of the stigma and is composed of polysaccharides, proteins, and lipids.…”

Section: Introductionmentioning

confidence: 99%

Lipid Transfer Proteins Enhance Cell Wall Extension in Tobacco

et al. 2005

View full text Add to dashboard Cite

Plant cells are enclosed by a rigid cell wall that counteracts the internal osmotic pressure of the vacuole and limits the rate and direction of cell enlargement. When developmental or physiological cues induce cell extension, plant cells increase wall plasticity by a process called loosening. It was demonstrated previously that a class of proteins known as expansins are mediators of wall loosening. Here, we report a type of cell wall-loosening protein that does not share any homology with expansins but is a member of the lipid transfer proteins (LTPs). LTPs are known to bind a large range of lipid molecules to their hydrophobic cavity, and we show here that this cavity is essential for the cell wall-loosening activity of LTP. Furthermore, we show that LTP-enhanced wall extension can be described by a logarithmic time function. We hypothesize that LTP associates with hydrophobic wall compounds, causing nonhydrolytic disruption of the cell wall and subsequently facilitating wall extension.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipid Transfer Proteins Enhance Cell Wall Extension in Tobacco

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Analysis of the dry stigmas of other crucifers (Heslop-Harrison and Shivanna, 1977) has indicated that the surface of each papillar cell is covered by an interrupted layer of cutin, called the cuticle, and a superficial proteinaceous pellicle layer of poorly defined molecular composition (Gaude and Dumas, 1986;Hiscock et al, 2002), which may function in the adhesion and hydration of pollen grains through interactions with components of the pollen surface (Stead et al, 1980;Gaude and Dumas, 1986;Zinkl et al, 1999). For their part, cells of the transmitting tract that line the path of pollen tube growth are thought to produce molecules that contribute to the rapid rate and directionality of tube elongation (Sanchez et al, 2004). Despite the importance of these cells, however, the mechanisms that underlie successful pollen-pistil interactions are poorly understood.…”

mentioning

confidence: 99%

Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth

et al. 2005

View full text Add to dashboard Cite

Plant reproductive development is dependent on successful pollen-pistil interactions. In crucifers, the pollen tube must breach the stigma surface and burrow through the extracellular matrix of the stigma epidermal cells and transmitting tract cells before reaching its ovule targets. The high degree of specificity in pollen-pistil interactions and the precision of directional pollen tube growth suggest that signals are continually being exchanged between pollen/pollen tubes and cells of the pistil that line their path. However, with few exceptions, little is known about the genes that control these interactions. The specialized functions of stigma epidermal cells and transmitting tract cells are likely to depend on the activity of genes expressed specifically in these cells. In order to identify these genes, we used the Arabidopsis (Arabidopsis thaliana) ATH1 microarray to compare the whole-genome transcriptional profiles of stigmas and ovaries isolated from wild-type Arabidopsis and from transgenic plants in which cells of the stigma epidermis and transmitting tract were specifically ablated by expression of a cellular toxin. Among the 23,000 genes represented on the array, we identified 115 and 34 genes predicted to be expressed specifically in the stigma epidermis and transmitting tract, respectively. Both gene sets were significantly enriched in predicted secreted proteins, including potential signaling components and proteins that might contribute to reinforcing, modifying, or remodeling the structure of the extracellular matrix during pollination. The possible role of these genes in compatible and incompatible pollen-pistil interactions is discussed.

show abstract

“…Within the ovary, the tube grows toward a receptive ovule, enters the ovule through the micropyle, and delivers the sperm cells within the FG to effect double fertilization. The genetic programs that govern the development of the MG and its interactions with the sporophyte are reviewed elsewhere in this issue (Edlund et al, 2004;McCormick, 2004;Kao and Tsukamoto, 2004;Sanchez et al, 2004).…”

Section: Introductionmentioning

confidence: 99%