Objective: To study the improvement of hand performance and play behavior in children with developmental disabilities using remodeled glove puppetry. Method: A pretest-posttest design was used in this randomized controlled trial to evaluate the intervention. Sixty-two children with developmental disabilities were randomly divided into experimental and control groups (n=31 each). Both groups underwent puppet play sessions once daily in playrooms. The children in the experimental group underwent rehabilitation by playing with the remodeled glove puppetry for 12 weeks, while the children in the control group played with non-remodeled glove puppetry. The Chinese puppet was remodeled using the Lego EV3® robot. We analyzed the motion kinematics of the hand using Siliconcoach® Pro 7 software and measured the force produced by the Baseline® hydraulic pinch gauge. The revised Knox Preschool Play Scale was used to score the play behavior. Results: Improvement in hand kinematics and play scales in the experimental group indicated that glove puppetry remodeled with EV3® robots had a good training effect. Discussion: Remodeled glove puppetry improved the range of motion and the qualities of play behavior, such as material management and participation of play quality. Further studies using remodeled glove puppetry with different disability populations are needed.