Pit pattern and three-dimensional configuration of isolated crypts from the patients with colorectal neoplasm

Abstract

Evaluation of the three-dimensional configuration revealed that when the pit patterns, of each isolated crypt differed, their three-dimensional configurations also differed. Recognition of the differences in the three-dimensional configuration should contribute toward both an understanding of pit-pattern diagnosis and the further development of the endoscopic diagnosis of various colorectal lesions.

Morphogenesis of a colorectal neoplasm with a type IIIS pit pattern inferred from isolated crypts

“…The orifi ces of the colonic gland can be elegantly visualized by SEM, but it is not possible to evaluate the deeper part of the glands without crypt isolation. Tamura et al 11 investigated and reported the three-dimensional structure of gland ducts corresponding to different pit patterns by using duct isolation.…”

“…21,22 The fi ndings of pit pattern studies have led to questions from endoscopic physicians regarding the relationship between the pits and the three-dimensional shape of the gland ducts. Tamura et al 11 examined the threedimensional structure of pit patterns using SEM. 11 This method, which involves much higher magnifi cation than is possible with endoscopy, is very useful for the investigation of tumor gland morphology.…”

“…Tamura et al 11 examined the threedimensional structure of pit patterns using SEM. 11 This method, which involves much higher magnifi cation than is possible with endoscopy, is very useful for the investigation of tumor gland morphology. A recent investigation described the SEM appearance of the surface structures of colonic adenomas and compared them with endoscopic and microscopic pit patterns.…”

“…8 However, the increasing number of cases diagnosed by magnifying videoendoscope has led to questions regarding the nature of the relationship between the pit pattern and the three-dimensional confi guration of the neoplastic gland. Using a crypt isolation method and an electron microscope, Tamura et al 11 investigated this relationship and showed that the shape of each isolated crypt corresponded to its pit pattern.…”

Evaluation of tumor development from the viewpoint of the three-dimensional configuration of isolated crypts in colorectal adenomas with a type IIIl pit pattern

“…4 However, in the type-IIIl pit pattern, the structural differences between superficial-type and protruded-type tumor are still obscure. We investigated isolated crypts, using an HCl-digestion method, 5 in LST nongranular type 1 (LST-NG) superficial tumors and Background.…”