Pitavastatin nanoparticle-engineered endothelial progenitor cells repair injured vessels

Abstract

Endothelial progenitor cells (EPC) participate in vessel recovery and maintenance of normal endothelial function. Therefore, pitavastatin-nanoparticles (NPs)-engineered EPC may be effective in repairing injured vasculature. Pitavastatin-loaded poly(lactic-co-glycolic) acid (PLGA) NPs were obtained via ultrasonic emulsion solvent evaporation with PLGA as the carrier encapsulating pitavastatin. The effects and mechanism of pitavastatin-NPs on EPC proliferation in vitro were evaluated. Then, EPC that internalized pitavastatin-NPs were transplanted into rats after carotid artery injury. EPC homing, re-endothelialization, and neointima were evaluated by fluorescence labeling, evans Blue and hematoxylin/eosin (H&E) staining. Pitavastatin-NPs significantly improved EPC proliferation compared with control and pitavastatin group. Those effects were blocked by pretreatment with the pharmacological phosphoinositide 3-kinase (PI3K) blockers LY294002. After carotid artery injury, more transplanted EPC were detected in target zone in Pitavastatin-NPs group than pitavastatin and control group. Re-endothelialization was promoted and intimal hyperplasia was inhibited as well. Thus, pitavastatin-NPs promote EPC proliferation via PI3K signaling and accelerate recovery of injured carotid artery.

Characterization of Phenotypic and Genotypic Diversity of Stenotrophomonas maltophilia Strains Isolated From Selected Hospitals in Iran

“…S. maltophilia strains are well - known biofilm-producing organisms with ability to adhere to biotic and abiotic surfaces (Pompilio et al., 2008). Few genes associated with biofilm formation in S. maltophilia have been experimentally studied (Liu et al., 2017). More recently, the correlation between mutations in rpfF and rmlA genes, encoding enoyl-CoA hydratase and glucose-1-phosphate thymidyltransferase, respectively, and the less extensive biofilm formation have been reported (Huang et al., 2006; Fouhy et al., 2007).…”

Nanomagnetite-embedded PLGA Spheres for Multipurpose Medical Applications

“…The successful experimental use of unconventional pharmaceutical formulations based on this particular PLGA copolymer was reported in modern strategies for diabetes-related conditions [21,22], HIV management [23,24], vaccine therapy [25], therapeutic angiogenesis [26,27], and tissue engineering [28,29,30]. Relevant examples of PLGA-based anti-infective strategies include treatment of streptococcal infections [31], ocular infection management [32], treatment of vaginal infection [33], and systemic antibiotherapy [34].…”

Polymers and Nanoparticles for Statin Delivery: Current Use and Future Perspectives in Cardiovascular Disease

“…In the preclinical field of vascular surgery, the group of Liu et al [ 94 , 95 ] performed interesting studies on models of carotid artery injury. Pitavastatin-loaded nanoparticles with PLGA improved endothelial progenitor cells proliferation and were more effective in repairing injured vasculature via the PI3K signaling pathway, by promoting re-endothelialization and reducing intimal hyperplasia [ 95 ].…”

“…In the preclinical field of vascular surgery, the group of Liu et al [ 94 , 95 ] performed interesting studies on models of carotid artery injury. Pitavastatin-loaded nanoparticles with PLGA improved endothelial progenitor cells proliferation and were more effective in repairing injured vasculature via the PI3K signaling pathway, by promoting re-endothelialization and reducing intimal hyperplasia [ 95 ]. Similarly, rosuvastatin-loaded nanoparticles with poly(L-lactide-co-caprolactone) (PLCL) favored endothelialization and reduced thrombotic potential through increased vascular endothelial growth factor (VEGF) signaling [ 94 ].…”