In this paper, synchronous analysis based on wavelet transform is applied to genomic sequences. To focus on the particular feature of periodicity 3 in the protein-coding region of genes, a coding method is applied on the sequence, which will be segmented to form a pitch synchronous representation. This modelling concept captures period of period fluctuation of signals. The wavelet transform enhances the periodicity, and a given threshold is used to make decision about exon's positions. The algorithm simulation shows the accuracy of the method in simultaneously locating exons and revealing the reading frame associated.