Pitfalls in the application of digoxin determinations

Shapiro, Barry A.; Kollmann, George; Heine, W

doi:10.1016/s0001-2998(75)80010-9

Cited by 10 publications

(3 citation statements)

References 28 publications

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“…The accurate detection of digoxin in biological fluids such as blood and urine is not an easy task due to its low concentration and generally complex biological matrices. Different techniques have been used to determine digoxin in blood and urine including the enzyme-multiplied immunoassay technique 36 , fluorescence polarisation immunoassay 37 , high performance liquid chromatography (HPLC) and radioimmune assay (RIA) 38 Some of these methods are relatively tedious and have been largely replaced by RIA which is more practical. Although, RIA is sensitive and commonly used in clinical and non-clinical studies, it has specificity problems such as cross-reactions with digoxin metabolites and endogenous digoxin-like substances are known to occur.…”

Section: Introductionmentioning

confidence: 99%

“…34,35 The accurate detection of digoxin in biological uids such as blood and urine is not an easy task due to its low concentration and generally complex biological matrices. Different techniques have been used to determine digoxin in blood and urine including the enzyme-multiplied immunoassay technique, 36 uorescence polarisation immunoassay, 37 high performance liquid chromatography (HPLC) and radioimmune assay (RIA) 38 or uorescence detection and LC/MS 39 or LC/MS/MS assay. 40 Some of these methods are relatively tedious and have been largely replaced by RIA which is more practical.…”

Section: Introductionmentioning

confidence: 99%

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Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

2015

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In this paper we used a modified fluorine-doped tin oxide (FTO) as the substrate of a novel aptasensor for electrochemical determination of digoxin. For this purpose, a selective thiolated digoxin aptamer was immobilized onto the gold nanoparticles deposited FTO (GNPs/FTO) surface. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the characterization of modified surfaces. The aptasensor response was based on decrease in the methylene blue (MB) current, as an electrochemical probe, after incubating with digoxin. Digoxin can be determined in linear concentration range from 0.02 to 0.2 µg/L by differential pulse voltammetry (DPV). The detection limit of the proposed aptasensor was 0.01 µg/L. Also, the fabricated aptasensor was applied to determine digoxin in urine and blood plasma samples with satisfactory results. Furthermore, high sensitivity and specificity, low detection limit and fast response, can make it possible to determine trace amounts of digoxin for routine clinical analysis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

2015

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show abstract

“…Different techniques have been used to determine concentrations of digoxin in blood and urine, including radioimmunoassay (RIA) [5], the enzyme-multiplied immunoassay technique (EMIT) [6], fluorescence polarisation immunoassay (FPIA) [7], highperformance liquid chromatography (HPLC) assay with RIA [8] or fluorescence detection and LC/MS [9] or LC/MS/MS assay [10]. Some of these methods are relatively tedious and have been largely replaced by RIA which is more practical.…”

Section: Introductionmentioning

confidence: 99%