Pitfalls in the Path of Quantitative Assessment of the Severity of Oncological Lesions in Diagnostic Nuclear Medicine

Suborgan Level Quantitation of Proteins in Tissues Delivered by Polymeric Nanocarriers

“…45 Nuclear imaging approaches also face challenges in quantification due to a variety of artifacts caused by the instrumentation and reconstruction algorithms that are used, resulting in the techniques being most commonly used for the relative quantitation of therapeutics in diseased vs healthy tissues. 46 In addition, nuclear imaging techniques have stringent safety requirements, relatively modest spatial resolution (∼1 mm), few readily available sources of radioisotopes, and a lack of broad accessibility to instrumentation. 44 Hence, better methods that can more readily quantitate protein therapeutics with a higher spatial resolution in animals are needed.…”

“…Fluorescence signals are influenced by the local microenvironment of the fluorophore, and this characteristic can sometimes lead to challenges in achieving absolute quantification in fluorescence imaging . Nuclear imaging approaches also face challenges in quantification due to a variety of artifacts caused by the instrumentation and reconstruction algorithms that are used, resulting in the techniques being most commonly used for the relative quantitation of therapeutics in diseased vs healthy tissues . In addition, nuclear imaging techniques have stringent safety requirements, relatively modest spatial resolution (∼1 mm), few readily available sources of radioisotopes, and a lack of broad accessibility to instrumentation .…”