Pitfalls in the performance of real time PCR tests for SARS CoV-2 and time to improve these tests

Bhatia, Sudhir

doi:10.21608/mid.2023.239655.1630

Microbes and Infectious Diseases

2023

DOI: 10.21608/mid.2023.239655.1630

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Pitfalls in the performance of real time PCR tests for SARS CoV-2 and time to improve these tests

Sudhir Bhatia

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2024

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Cited by 2 publications

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Defining the Quality Standards to Use and Cite PCR Primers and Probes from Literature

Bhatia

2024

Med Sci Discov

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Objective: Polymerase chain reaction (PCR) primers and probes from the medical literature are frequently used in current research and diagnostics, contributing to numerous publications. Our previous analysis revealed that many of these oligonucleotides fail to effectively detect all strains of various pathogens, leading to questionable results in published studies. Therefore, our goal is to establish quality standards for the use and citation of such primers and probes from the literature. Materials and Methods: We analyzed approximately 500 publications from PubMed and other sources over the past 20 years. In many cases, in-silico analysis was performed, while in others, primers and probes were tested in laboratory settings. Results: We identified numerous instances where outdated oligonucleotides from the literature are still being used, raising concerns about their ability to detect diverse pathogen strains. Authors utilizing these outdated primers and probes must provide evidence of their continued efficacy. Peer reviewers should also give this issue careful attention during the review process. In several cases, we redesigned and updated these oligos for use in our laboratory and in commercial kits. Conclusion: We propose preliminary guidelines to address the use of outdated primers and probes, aiming to prevent questionable publications that result in the wastage of taxpayer funds. Addressing this issue is critical to producing high-quality results that can contribute to the development of new diagnostics, vaccines, and therapies.

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Defining the Quality Standards to Use and Cite PCR Primers and Probes from Literature

Bhatia

2024

Med Sci Discov

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How to prepare for an avian influenza H5N1 pandemic outbreak: Lessons learned from the influenza H1N1 pandemic of 1918

Sudhir

2024

Sanamed

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In this paper, we highlight the significant challenges encountered during the influenza H1N1 pandemic of 1918. These lessons are being utilized to prepare for potential avian influenza H5N1 pandemic outbreaks, as this virus will impact both humans and food-serving animals. Preparation for this threat is crucial because H5N1 infections are already affecting the United States and many other countries.

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Pitfalls in the performance of real time PCR tests for SARS CoV-2 and time to improve these tests

Cited by 2 publications

References 6 publications

Defining the Quality Standards to Use and Cite PCR Primers and Probes from Literature

Defining the Quality Standards to Use and Cite PCR Primers and Probes from Literature

How to prepare for an avian influenza H5N1 pandemic outbreak: Lessons learned from the influenza H1N1 pandemic of 1918

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