“…[2] , and it is easy to be remained in the food of poultry which can do harm to the health of the consumers.Therefore, many countries and regions have set the limit of the remainder of the Gentamicin, the European Union specified that the limit of Gentamicin residue was 50 µg/kg [3] . In China Pharmacopoeia, the determination method is the high performance liquid chromatography [4][5][6] , in addition, there are the microorganism method [7][8] , the spectrophotometry [9][10] , the fluorescence analysis [11][12] , the ELISA [13][14] , the colorimetric method [15][16] , the capillary electrophoresis method [17][18] and so on. At present, fluorescence analysis method for its high sensitivity, simple, reproducible and other advantages, has been widely used in the detection of the food, environment, medicine and other areas [19][20][21] .…”