Pituitary adenylate cyclase activating peptide-27 – like immunoreactive nerve fibers in the mucosal layer of canine gastrointestinal tract in physiology and during inflammatory bowel disease

Gonkowski, Sławomir; Rychlik, Andrzej; Całka, Jarosław

doi:10.2478/bvip-2013-0065

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…The fragments of the colonic mucosal layer were then frozen at -20 8C, cut to 10-mm sections with a Microm cryostat (HM525, Walldorf, Germany) and mounted on microscopic slides. The sections were examined by the single immunofluorescence technique according to Gonkowski et al (2013). Briefly, the procedure was as follows: (a) drying for 45 min at room temperature (rt), (b) incubation with blocking solution (10% goat serum, 0.1% bovine serum albumin, 0.01% NaN 3 , Triton X-100 and thiomersal in PBS) for 1 h to avoid non-specific binding of antibody, (c) incubation with anti-SP antibody (rat, Biogenesis Ltd, Poole, UK, catalogue no.…”

Section: Methodsmentioning

confidence: 99%

“…The above-mentioned signs are accompanied by histopathological changes in the intestinal wall. In recent times, attention has been focused on the participation of intestinal innervation in pathological processes connected with IBD (Gonkowski et al, 2013;Rychlik et al, 2017;Brinkman et al, 2019), but the roles of particular neuronal active substances in the development of this disease are still not clear.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the expression of substance P in nerve fibres of the colonic mucosa in dogs suffering from inflammatory bowel disease

Rychlik

Gonkowski

Makowska

et al. 2020

AVet

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Due to its difficult diagnosis and complicated treatment, inflammatory bowel disease (IBD) in dogs is a challenge for the veterinarian. Several aspects connected with pathological changes during IBD still remain unknown. Since one of these aspects is the participation of intestinal innervation in the evolution of the disease, the aim of this study was to demonstrate changes in the number and distribution of intramucosal colonic nerve fibres immunoreactive to substance P (SP) arising as the disease progresses. SP is one of the most important neuronal factors in intestinal innervation which, among other tasks, takes part in the conduction of pain stimuli. Using routine immunofluorescence technique, the density of nerve fibres containing SP was evaluated within mucosal biopsy specimens collected from the descending colon of healthy dogs and animals suffering from IBD of varying severity. The results of the study indicate that during severe IBD the number of nerve fibres containing SP located in the colonic mucosal layer increases in comparison to control animals. The number of SP-positive intramucosal nerves amounted to 10.99 ± 2.11 nerves per observation field in healthy dogs, 14.62 ± 2.86 in dogs with mild IBD, 14.80 ± 0.91 in dogs with moderate IBD and 19.03 ± 6.11 in animals with severe IBD. The observed changes were directly proportional to the intensity of the disease process. These observations may suggest a role of this neuronal substance in pathological processes occurring during IBD. Although the exact mechanism of the observed changes has not been completely explained, the results obtained in this investigation may contribute to improving the diagnosis and treatment of this disease, as well as the staging of canine IBD in veterinary practice.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in the expression of substance P in nerve fibres of the colonic mucosa in dogs suffering from inflammatory bowel disease

Rychlik

Gonkowski

Makowska

et al. 2020

AVet

Self Cite

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“…Results are presented as means ± standard error of mean (SEM). Nerve fiber distribution was assessed under × 40 objective by subjective observation (two independent researchers) as previously described (Gonkowski et al 2013 ) and depending on the density of labeling, described as not found or very few, small, moderate, and large number of nerve fibers.…”

Section: Methodsmentioning

confidence: 99%

Origins and Neurochemical Complexity of Preganglionic Neurons Supplying the Superior Cervical Ganglion in the Domestic Pig

Juranek

Wojtkiewicz

2014

J Mol Neurosci

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The superior cervical ganglion (SCG) is a center of sympathetic innervation of all head and neck organs. SCG sympathetic preganglionic neurons (SPN) were found in the nucleus intermediolateralis pars principalis (IMLpp), the nucleus intermediolateralis pars funicularis (IMLpf), the nucleus intercalatus spinalis (IC), and the nucleus intercalatus spinalis pars paraependymalis (ICpe). Despite its importance, little is known of SCG innervation and chemical coding in the laboratory pig, a model that is physiologically and anatomically representative of humans. Here in our study, we established the distribution and chemical coding of Fast Blue (FB) retrogradely labelled SPN innervating porcine SCG. After unilateral injection of FB retrograde tracer into the left SCG, labeled neurons were found solely on the ipsilateral side with approximately 98 % located in Th1–Th3 segments and predominantly distributed in the IMLpp and IMLpf. Neurochemical analysis revealed that approximately 80 % of SPN were positive both to choline acetyltransferase (ChAT) and nitric oxide synthase (NOS) and were surrounded by a plethora of opioidergic and peptiergic nerve terminals. The results of our study provide a detailed description of the porcine preganglionic neuroarchitecture of neurons controlling the SCG, setting the stage for further studies concerning SPN plasticity under experimental/pathological conditions.

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“…The specimens were transferred to 18% phosphate-buffered sucrose solution with a temperature of 4 °C. Frozen sections of 10 µm were cut using a Microm cryostat (HM525, Walldorf, Germany), placed on gelatinised microscopic slides and subjected to single-cell immunofluorescence staining in accordance with the method reported by Gonkowski et al (2013b). The specimens were dried for 45 min at room temperature and rinsed three times in buffered NaCl solution (PBS, 0.1 mol, pH 7.4) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Cocaine- and amphetamine-regulated transcript immunoreactive nerve fibres in the mucosal layer of the canine gastrointestinal tract under physiological conditions and in inflammatory bowel disease

et al. 2015

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ABSTRACT:The objective of this study was to determine the effect of canine inflammatory bowel disease (IBD) on the density of cocaine-and amphetamine-regulated transcript (CART) immunoreactive nerve fibres in the mucosa of different sections of the gastrointestinal tract. Fibre density was analysed in mucosal specimens from the duodenum, jejunum and descending colon of healthy dogs and patients with inflammatory bowel disease of varying intensity. Nervous fibres were stained in single-cell immunofluorescence assays. The density of CARTimmunoreactive fibres was determined using a semi-quantitative approach by counting the number of fibres in the field of view (0.1 mm 2 ). An increase in the density of CART-immunoreactive fibres was observed in dogs with moderate and severe IBD in comparison with healthy subjects. The results suggest that the presence of CART in enteric nerve fibres could play a role in the pathogenesis and development of canine IBD.

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Pituitary adenylate cyclase activating peptide-27 – like immunoreactive nerve fibers in the mucosal layer of canine gastrointestinal tract in physiology and during inflammatory bowel disease

Cited by 5 publications

References 33 publications

Changes in the expression of substance P in nerve fibres of the colonic mucosa in dogs suffering from inflammatory bowel disease

Changes in the expression of substance P in nerve fibres of the colonic mucosa in dogs suffering from inflammatory bowel disease

Origins and Neurochemical Complexity of Preganglionic Neurons Supplying the Superior Cervical Ganglion in the Domestic Pig

Cocaine- and amphetamine-regulated transcript immunoreactive nerve fibres in the mucosal layer of the canine gastrointestinal tract under physiological conditions and in inflammatory bowel disease

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