Pituitary‐dependent expression of the testicular angiotensin II receptor and its subtypes in rats

Hirai, Kei; Song, Keifu; Kanehara, H; Shiota, Naotaka; Ueda, Haruhiko; Kim, S; Miyazaki, Hitoshi; Katsuoka, Yoji; Miyazaki, Mizuo

doi:10.1046/j.1365-2605.1998.00102.x

Cited by 7 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aguilera et al (7) reported also a decrease in ANG II binding during development and that in immature rat a high proportion of ANG II receptors in the testes are located in nondifferentiated mesenchymal cells of the interstitium. Hypophysectomy led to a marked increase in AT 1 and AT 2 receptor concentration of rat testis with predominant occurrence of AT 1 receptors measured by in vitro autoradiography (275). The concentration of rat AT 1 and AT 2 receptors at protein level is dependent on the developmental stage.…”

Section: Male Reproductive Tractmentioning

confidence: 92%

See 1 more Smart Citation

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,512

1,371

View full text Add to dashboard Cite

Since the first identification of renin by Tigerstedt and Bergmann in 1898, the renin-angiotensin system (RAS) has been extensively studied. The current view of the system is characterized by an increased complexity, as evidenced by the discovery of new functional components and pathways of the RAS. In recent years, the pathophysiological implications of the system have been the main focus of attention, and inhibitors of the RAS such as angiotensin-converting enzyme (ACE) inhibitors and angiotensin (ANG) II receptor blockers have become important clinical tools in the treatment of cardiovascular and renal diseases such as hypertension, heart failure, and diabetic nephropathy. Nevertheless, the tissue RAS also plays an important role in mediating diverse physiological functions. These focus not only on the classical actions of ANG on the cardiovascular system, namely, the maintenance of cardiovascular homeostasis, but also on other functions. Recently, the research efforts studying these noncardiovascular effects of the RAS have intensified, and a large body of data are now available to support the existence of numerous organ-based RAS exerting diverse physiological effects. ANG II has direct effects at the cellular level and can influence, for example, cell growth and differentiation, but also may play a role as a mediator of apoptosis. These universal paracrine and autocrine actions may be important in many organ systems and can mediate important physiological stimuli. Transgenic overexpression and knock-out strategies of RAS genes in animals have also shown a central functional role of the RAS in prenatal development. Taken together, these findings may become increasingly important in the study of organ physiology but also for a fresh look at the implications of these findings for organ pathophysiology.

show abstract

Section: Male Reproductive Tractmentioning

confidence: 92%

“…Hirai et al (275) could verify that the AT 1 and AT 2 mRNA gene expression in rat testis is pituitary dependent. After hypophysectomy, the receptor gene expression was significantly increased.…”

Section: Male Reproductive Tractmentioning

confidence: 99%

Physiology of Local Renin-Angiotensin Systems

Paul

Mehr²,

Kreutz³

2006

Physiological Reviews

1,512

1,371

View full text Add to dashboard Cite

show abstract

“…The components of the renin angiotensin system have long been known to be expressed in the mammalian testis (34,35), and their involvement in the development of rat testis has also been documented (36). The AT2 receptor is present, as the major receptor subtype, during the neonatal stage of testicular development and it subsequently shifts to AT1 subtype during puberty (37).…”

Section: Discussionmentioning

confidence: 99%

Angiotensin II Stimulates Contraction and Growth of Testicular Peritubular Myoid Cellsin Vitro

et al. 2002

View full text Add to dashboard Cite

Seminiferous tubule contraction, an important step in the regulation of spermatogenesis and testicular sperm output, is regulated by several agonists. In the present paper, we investigated whether angiotensin II (Ang II) may have a place among them. In binding experiments performed to assess the presence of specific receptors in rat peritubular myoid cells (TPMC), binding of (125)I-Ang II to TPMC was saturable in a time-dependent manner. Competition binding experiments performed with Losartan and PD 123319 showed that Losartan was able to inhibit the binding of (125)I-Ang II, whereas PD 123319 was ineffective. Ang II induced a dose-dependent rise in intracellular Ca(2+). Depletion of intracellular calcium stores by thapsygargin resulted in a lower rise of intracellular calcium, and the L-type voltage-operated calcium channel (VOCC-L) blocker verapamil abolished the Ca(2+) influx in rat TPMC. Altogether, these findings indicate that the Ang II-induced increase in [Ca(2+)](i) involves both extracellular influx and Ca(2+) release from intracellular stores. Ang II induced a dose-dependent TPMC contraction, and Losartan and not PD 123319 inhibited the response. Ang II-induced contraction was inhibited by adrenomedullin, previously shown to antagonize endothelin 1-provoked contraction in those cells. Ang II elicited (3)H-thymidine DNA incorporation and proliferation in a dose-dependent manner in TPMC. Losartan and both MAPK inhibitor PD 98059 and tyrosine kinase inhibitor AG18 were able to inhibit Ang II-induced (3)H-thymidine uptake and cell proliferation. In conclusion, the present study documents that angiotensin II, the active mediator of the tissue and circulating renin-angiotensin system present in the mammalian testis, induces contraction, growth and rise in intracellular calcium in rat peritubular myoid cells via angiotensin II type 1 receptors, and suggests that Ang II is involved in the paracrine regulation of the seminiferous tubule function.

show abstract

“…Ang II has been reported to be capable of inhibiting adenylate cyclase activity in rat Leydig cells, reducing basal and gonadotropin-stimulated cAMP and testosterone production [53,54]. On the other hand, it has also been proposed that Ang II may play a role in testicular growth and/or differentiation [55]. Finally, the Ang II receptors AT1R and AT2R have also been detected in rat, monkey and human testes, specifically in Leydig cells [53,56,57].…”

Section: The Renin/ace/ang Ii/at1r/at2r Axismentioning

confidence: 99%

Regulation of Male Fertility by the Renin-Angiotensin System

Citores

Subirán

2020

IJMS

View full text Add to dashboard Cite

The renin-angiotensin system (RAS) is a peptidic system known mainly for its roles in the maintenance of blood pressure and electrolyte and fluid homeostasis. However, several tissues and cells have been described to possess an intrinsic RAS that acts locally through different paracrine and autocrine mechanisms. In the male reproductive system, several components of this system have been observed in various organs and tissues, such as the testes, spermatozoa and seminal fluid. Some functions attributed to this local RAS are maintenance of seminal plasma electrolytes, regulation of steroidogenesis and spermatogenesis, and sperm functions. However, their specific actions in these locations are not fully understood. Therefore, a deep knowledge of the functions of the RAS at both the testicular and seminal levels could clarify its roles in male infertility and sperm physiology, and the different RAS elements could be used to design tools enabling the diagnosis and/or treatment of male infertility.

show abstract

Pituitary‐dependent expression of the testicular angiotensin II receptor and its subtypes in rats

Cited by 7 publications

References 37 publications

Physiology of Local Renin-Angiotensin Systems

Physiology of Local Renin-Angiotensin Systems

Angiotensin II Stimulates Contraction and Growth of Testicular Peritubular Myoid Cellsin Vitro

Regulation of Male Fertility by the Renin-Angiotensin System

Contact Info

Product

Resources

About