Pituitary Hormone-producing Cells After Estradiol Application in Rat Models of Menopause

Milošević, Verica; Ajdžanović, Vladimir

doi:10.2478/sjecr-2014-0015

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…Estradiol is able to increase follicular atresia either indirectly, through the inhibition of gonadotropins [54], or directly through the increase of oxidative stress, as indicated in our results obtained by determining the anti-oxidative enzyme activity. Because of its maturing effect [55], through the PRL synthesis and secretion affirmation, estradiol increased not only the number, but also the activity of mCL, which resulted in an increase of progesterone concentration in serum.…”

Section: Discussionmentioning

confidence: 64%

Effects of soy phytoestrogens on pituitary-ovarian function in middle-aged female rats

et al. 2015

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The aim of this study was to assess the effects of genistein (G) and daidzein (D) on the histological, hormonal, and functional parameters of the pituitary-ovarian axis in middle-aged female rats, and to compare these effects with the effects of estradiol (E), commonly used in the prevention and treatment of menopausal symptoms. Middle-aged (12 month old) Wistar female rats subcutaneously received 35 mg/kg of G, or 35 mg/kg of D, or 0.625 mg/kg of E every day for 4 weeks. Each of the treated groups had a corresponding control group. An intact control group was also established. G and D did not change the intracellular protein content within gonadotropic and lactotropic cells, but vacuolization was observed in all the cell types. In contrast, E caused an inhibition of gonadotropic and stimulation of lactotropic cells. Also, ovaries of middle-aged female rats exposed to G or D have more healthy primordial and primary follicles and less atretic follicles. E treatment in the ovaries had a mostly negative effect, which is reflected by the increased number of atretic follicles in all tested classes. G and D provoked decrease in CuZnSOD and CAT activity, while E treatment increased MnSOD and decreased CuZnSOD and GSHPx activity. All the treatments increased serum estradiol and decreased testosterone levels, while D and E increased the serum progesterone level. In conclusion, soy phytoestrogens exhibited beneficial effects on pituitary-ovarian function in middle-aged female rats, as compared to estradiol.

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Section: Discussionmentioning

confidence: 64%

Effects of soy phytoestrogens on pituitary-ovarian function in middle-aged female rats

et al. 2015

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Effect of selective serotonin reuptake inhibitors (SSRIs) versus estradiol administration on anterior pituitary cell population and possible regenerative capacity in ovariectomized adult albino rats

Hanna

Malak

Amin

et al. 2018

The Egyptian Journal of Anatomy

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Background: Reproductive ageing in women and the hormonal changes that occur during the onset of human menopause have been ascribed solely to ovarian failure and oocyte depletion. However, some studies have shown the central nervous system to be the major regulator of age-related reproductive dysfunction. Moreover, it has been postulated that the pituitary gland plays a key role in the menopausal process. Estradiol is a form of estrogen, a female sex hormone produced by the ovaries. Estradiol is used to treat symptoms of menopause such as hot flashes, and vaginal dryness, burning, and irritation. On the other hand, selective serotonin reuptake inhibitors (SSRIs) ease depression by increasing levels of serotonin in the brain. Aim of work: Thus, it became the aim of the present study, to elucidate the effect of SSRIs versus estradiol in ovariectomized rats on the overall anterior pituitary cell population with special emphasis on the Folliculo-stellate (FS) cells. Material and Methods: Thirty adult female Albino rats, ageing 3-4 months and 140-170 gm body weight were used in the present study. They were divided into 4 groups as follows: Group A (control group): further subdivided into 2 subgroups each including 6 rats. A1: rats were left untreated throughout the experiment. A2: rats were left untreated then received 2ml subcutaneous (s.c.) sesame oil injection daily for one week prior to sacrifice. In the remaining 3 groups (6 rats each), the ovaries were ectomized and rats were left for five months to mimic estrogen-depletion models. Then; Group B: 6 rats were used as an ovariectomy model. Group C: 6 rats received 40µg/kg body weight estradiol by s.c. injection daily for one week prior to sacrifice. Group D: 6 rats received 10mg/kg fluoxetine at a single dose/day by feeding tube for one week prior to sacrifice. Results: In the ovariectomized rats, there was loss of structural organization and an apparent decrease of cell population. Widespread vacuolations and cellular degeneration were also observed. An improved cellular architecture and cell population within pars distalis were seen with the administration of both estradiol and fluoxetine. Semithin sections confirmed these results. Immunostaining for the protein S-100 showed an apparent decrease in the distribution of the protein S-100 expression within the FS cells in ovariectomized rats that was significantly increased in response to estradiol and fluoxetine. Conclusion: Ovariectomized rats revealed degeneration of the anterior pituitary cell population and decreased expression of protein S-100 in the pars distalis indicating decreased population of FS cells. Such effects were reversed by either estradiol or fluoxetine administration. This might suggest the added effect of both drugs if administered together on the improvement of anterior pituitary cell population as well as FC cells.

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Pituitary Hormone-producing Cells After Estradiol Application in Rat Models of Menopause

Cited by 2 publications

References 35 publications

Effects of soy phytoestrogens on pituitary-ovarian function in middle-aged female rats

Effects of soy phytoestrogens on pituitary-ovarian function in middle-aged female rats

Effect of selective serotonin reuptake inhibitors (SSRIs) versus estradiol administration on anterior pituitary cell population and possible regenerative capacity in ovariectomized adult albino rats

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