Pituitary Tumor Transforming Gene Binding Factor: A Novel Transforming Gene in Thyroid Tumorigenesis

Stratford, Anna L.; Boelaert, Kristien; Tannahill, Lesley; Kim, Dae S.; Warfield, Adrian; Eggo, Margaret C.; Gittoes, Neil; Young, Lawrence S.; Franklyn, Jayne A.; McCabe, Chris

doi:10.1210/jc.2005-0523

Cited by 53 publications

(111 citation statements)

References 37 publications

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“…Among these, there are pituitary tumor-transforming gene protein-binding factor PTTG1IP and ribonucleoprotein hnRNP F. PTTG1IP is a prognostic indicator in thyroid cancer, even if its precise contribution to tumorigenesis has not yet been explored. 61 Also, increased expression of hnRNP F has been seen in more aggressive colorectal tumors. This suggests hnRNP F as a potential marker for colorectal cancer progression.…”

Section: J Journal Of Proteome Researchmentioning

confidence: 99%

Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

et al. 2007

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Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used twodimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which G 2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

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Section: J Journal Of Proteome Researchmentioning

confidence: 99%

Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

et al. 2007

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“…NIH3T3 cells stably transfected with wild-type PTTG also demonstrated increased PBF expression. Conversely, PTTG expression was not stimulated in PBF stable cell lines (Stratford et al 2005).…”

Section: Pbf In Thyroid Cancermentioning

confidence: 85%

“…PBF expression was found to be significantly increased in thyroid cancer and independently associated with early tumour recurrence (Stratford et al 2005). When stratified by tumour type, there was no significant difference in PBF expression between papillary (nZ17) and follicular (nZ7) carcinomas.…”

Section: Pbf In Thyroid Cancermentioning

confidence: 89%

“…When stratified by tumour type, there was no significant difference in PBF expression between papillary (nZ17) and follicular (nZ7) carcinomas. No mutations were observed when the coding region of PBF was sequenced in these 24 thyroid tumours (Stratford et al 2005). PBF expression was significantly and positively correlated with PTTG expression, and a potential contributing factor to this relationship was identified through subsequent in vitro studies, which found that over expression of PTTG in primary human thyroid cultures and PTTG-null HCT116 colorectal carcinoma cells significantly increased PBF expression.…”

Section: Pbf In Thyroid Cancermentioning

confidence: 90%

“…However, similar to PTTG, PBF demonstrates transforming ability and is overexpressed in a growing number of tumour types , Stratford et al 2005, Watkins et al 2010. Following its discovery, many of the subsequent studies of PBF have also assessed PTTG and attempted to delineate the contribution of each protein .…”

Section: Introductionmentioning

confidence: 99%

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Expression and function of the novel proto-oncogene PBF in thyroid cancer: a new target for augmenting radioiodine uptake

Franklyn¹,

McCabe²

2011

Journal of Endocrinology

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Pituitary tumor-transforming gene (PTTG)-binding factor (PBF; PTTG1IP) was initially identified through its interaction with the human securin, PTTG. Like PTTG, PBF is upregulated in multiple endocrine tumours including thyroid cancer. PBF is believed to induce the translocation of PTTG into the cell nucleus where it can drive tumourigenesis via a number of different mechanisms. However, an independent transforming ability has been demonstrated both in vitro and in vivo, suggesting that PBF is itself a proto-oncogene. Studied in only a limited number of publications to date, PBF is emerging as a protein with a growing repertoire of roles. Recent data suggest that PBF possesses a complex multifunctionality in an increasing number of tumour settings. For example, PBF is upregulated by oestrogen and mediates oestrogen-stimulated cell invasion in breast cancer cells. In addition to a possible role in the induction of thyroid tumourigenesis, PBF overexpression in thyroid cancers inhibits iodide uptake. PBF has been shown to repress sodium iodide symporter (NIS) activity by transcriptional regulation of NIS expression through the human NIS upstream enhancer and further inhibits iodide uptake via a post-translational mechanism of NIS governing subcellular localisation. This review discusses the current data describing PBF expression and function in thyroid cancer and highlights PBF as a novel target for improving radioiodine uptake and thus prognosis in thyroid cancer.

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Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

Martínez,

Rodriguez,

McCubbin

et al. 2024

Biotechnology Journal

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Chinese hamster ovary (CHO) cells are widely used to produce complex biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand for such products. One way to enhance productivity is by cultivating cells at high densities, but inhibitory by‐products, such as metabolite derivatives from amino acid degradation, can hinder achieving high cell densities. This research examines the impact of these inhibitory by‐products on high‐density cultures. We cultured X1 and X2 CHO cell lines in a small‐scale semi‐perfusion system and introduced a mix of inhibitory by‐products on day 10. The X1 and X2 cell lines were chosen for their varied responses to the by‐products; X2 was susceptible, while X1 survived. Proteomics revealed that the X2 cell line presented changes in the proteins linked to apoptosis regulation, cell building block synthesis, cell growth, DNA repair, and energy metabolism. We later used the AB cell line, an apoptosis‐resistant cell line, to validate the results. AB behaved similar to X1 under stress. We confirmed the activation of apoptosis in X2 using a caspase assay. This research provides insights into the mechanisms of cell death triggered by inhibitory by‐products and can guide the optimization of CHO cell culture for biopharmaceutical manufacturing.

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Pituitary Tumor Transforming Gene Binding Factor: A Novel Transforming Gene in Thyroid Tumorigenesis

Abstract: PBF is an additional prognostic indicator in differentiated thyroid cancer that is transforming in vitro and tumorigenic in vivo.

Cited by 53 publications

References 37 publications

Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

Expression and function of the novel proto-oncogene PBF in thyroid cancer: a new target for augmenting radioiodine uptake

Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells

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