2001
DOI: 10.1007/s004410100410
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Pivotal role of the interstitial cells of Cajal in the nitric oxide signaling pathway of rat small intestine

Abstract: The nitric oxide (NO) signaling pathway is a major nonadrenergic-noncholinergic transmitter mechanism in the enteric nervous system. Our aim was to localize the enzymes in question, i.e., neuronal nitric oxide synthase (nNOS), soluble guanylate cyclase (sGC), and cGMP-dependent kinase type I (cGK-I) in rat small intestine by indirect immunofluorescence. nNOS staining was found in neurons of the myenteric plexus and in varicose nerve fibers mainly in the circular muscle layer. The cells positive for neurokinin-… Show more

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Cited by 54 publications
(10 citation statements)
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“…Consistent with the present observations and idea that IMA activity may reflect the complex integration occurring in the IMA-ICC complexes, ICC pacemaking and activity has previously been shown to be modulated by catecholaminergic (Jun et al, 2004), nitrergic (Zhang et al, 2010), and cholinergic (Ward et al, 2000; Zhang et al, 2010) signals. Furthermore, and correspondingly, in different immunohistochemical experiments, these axonal phenotypes have also been previously observed in close apposition to ICCs: TH+ axons (Cobine et al, 2009), NOS+ neurites (Salmhofer et al, 2001; Cobine et al, 2009), and VAChT+ processes (Ward et al, 2000; Beckett et al, 2003). The present observations replicate these earlier structural observations, but, more importantly, also establish that one and the same ICCs are innervated by IMAs and the different efferent inputs.…”
Section: 1 Discussionsupporting
confidence: 57%
See 1 more Smart Citation
“…Consistent with the present observations and idea that IMA activity may reflect the complex integration occurring in the IMA-ICC complexes, ICC pacemaking and activity has previously been shown to be modulated by catecholaminergic (Jun et al, 2004), nitrergic (Zhang et al, 2010), and cholinergic (Ward et al, 2000; Zhang et al, 2010) signals. Furthermore, and correspondingly, in different immunohistochemical experiments, these axonal phenotypes have also been previously observed in close apposition to ICCs: TH+ axons (Cobine et al, 2009), NOS+ neurites (Salmhofer et al, 2001; Cobine et al, 2009), and VAChT+ processes (Ward et al, 2000; Beckett et al, 2003). The present observations replicate these earlier structural observations, but, more importantly, also establish that one and the same ICCs are innervated by IMAs and the different efferent inputs.…”
Section: 1 Discussionsupporting
confidence: 57%
“…Panels A and B illustrate the parallel strings of ICCs (Vector VIP stained) found in both circular (panel A) and longitudinal (panel B) that appear to serve as accessory tissue scaffolding for vagal IMAs. Panel C illustrates, as previously described (e.g., Salmhofer et al, 2001), NOS+ fibers (DAB, brown) are observed in tight association with ICCs (Vector VIP, violet); Panel D depicts, as has been previously reported (e.g., Ward et al, 2000 ), that cholinergic or VChAT+ fibers (DAB, brown) also appear to course so as to make contact with chains of ICCs (Vector VIP, violet); Panels E and F illustrate that catecholaminergic or TH+ axons (DAB, brown), as has been reported (e.g., Cobine et al, 2009), course in tight registration with the ICC scaffolds; Panels G and H illustrate the fact that (presumably visceral afferent) CGRP+ axons (DAB, brown) also course on or in conjunction with the chains of ICCs (Vector VIP, violet) with which vagal IMAs form complexes. Scale bars: Panel A: 40 μm, applies to panels A and B; Panel C: 20 μm, applies to panels C and D; Panel E: 25 μm, applies to panels E and G; Panel F: 20 μm, applies to panels F and H.…”
Section: Figurementioning
confidence: 94%
“…sGC is expressed in ICC-DMP of the rat small intestine [34] and almost all ICC in the guinea pig [20]. Activated sGC catalyses the conversion of GTP to cyclic GMP (cGMP), a second messenger in intracellular signaling cascades.…”
Section: Functional Investigations Of Icc and Neurotransmissionmentioning
confidence: 99%
“…Enhanced cGMP levels were blocked with the NO antagonist, L-nitroarginine. cGMP-dependent protein kinase I, which is activated by cGMP, was also expressed highly in ICC-DMP [34] in the rat small intestine and almost all ICC in the guinea pig (unpublished data). These observations show that ICC are functionally innervated by nitrergic inhibitory neurons.…”
Section: Functional Investigations Of Icc and Neurotransmissionmentioning
confidence: 99%
“…6 NO is a presumed neurotransmitter of the gastrointestinal tract, it can play an important role in the relaxation of the smooth muscles and is involved in many physiological and pathological processes. 79 The production of NO is indicated immunohistochemically by the demonstration of nitric oxide synthase (NOS), the enzyme responsible for NO generation.…”
Section: Introductionmentioning
confidence: 99%