Pivoting the Plant Immune System from Dissection to Deployment

Dangl, Jeffery L.; Horváth, Diána; Staskawicz, Brian J.

doi:10.1126/science.1236011

Cited by 1,056 publications

(897 citation statements)

References 93 publications

(92 reference statements)

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“…Timely activation of defense mechanisms requires pathogen detection using either cell surface receptors or intracellular immune receptors of the nucleotide-binding, leucine-rich repeat (NLR, aka NB-LRR or NBS-LRR) protein family [5][6][7] . Most disease resistance (R) genes encode NLR proteins, which directly or indirectly recognize pathogen effectors, and can carry TIR domain or a coiled-coil (CC) signaling domain at their N-termini (TIR-NLRs or CCNLRs) 5 .…”

mentioning

confidence: 99%

Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing

et al. 2016

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mentioning

confidence: 99%

Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing

et al. 2016

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“…Besides PRR-mediated basal resistance, plant genomes encode hundreds of intracellular nucleotide-binding and leucine-rich repeat (NB-LRR) immune receptors (also known as “R proteins”) to detect the presence of pathogen effectors delivered inside plant cells 10 . Individual or stacked R genes have been transformed into plants to confer effector-triggered immunity (ETI) 11, 12 . In addition to PRR and R genes, NPR1 is another favourite gene used in engineering plant resistance 4 .…”

mentioning

confidence: 99%

uORF-mediated translation allows engineered plant disease resistance without fitness costs

Yuan

et al. 2017

Nature

285

189

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Controlling plant disease has been a struggle for mankind since the advent of agriculture. Studies of plant immune mechanisms have led to strategies of engineering resistant crops through ectopic transcription of plants’ own defence genes, such as the master immune regulatory gene NPR11. However, enhanced resistance obtained through such strategies is often associated with significant penalties to fitness2, making the resulting products undesirable for agricultural applications. To remedy this problem, we sought more stringent mechanisms of expressing defence proteins. Based on our latest finding that translation of key immune regulators, such as TBF13, is rapidly and transiently induced upon pathogen challenge (accompanying manuscript), we developed “TBF1-cassette” consisting of not only the immune-inducible promoter but also two pathogen-responsive upstream open reading frames (uORFsTBF1) of the TBF1 gene. We demonstrate that inclusion of the uORFsTBF1-mediated translational control over the production of snc1 (an autoactivated immune receptor) in Arabidopsis (At) and AtNPR1 in rice enables us to engineer broad-spectrum disease resistance without compromising plant fitness in the laboratory or in the field. This broadly applicable new strategy may lead to reduced use of pesticides and lightening of selective pressure for resistant pathogens.

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“…Most of these approaches are focused on the family of genes containing NBS (nucleotide-binding site)/LRRs (Leucine-Rich Repeats)-domains which constitute an expanded group of plant receptor genes linked directly to defense reactions (Dangl et al 2013;Debener and Byrne 2014). In turn, LRR domains evolve novel ligand-binding specificities under strong positive selection with a wealth of different mechanisms, ranging from point mutations, variations in their repeat numbers and tandem gene duplications (Yang et al 2008;Zambounis et al 2012b).…”

Section: Introductionmentioning

confidence: 99%

Identification and evidence of positive selection upon resistance gene analogs in cotton (Gossypium hirsutum L.)

Zambounis

Ganopoulos

Kalivas³

et al. 2016

Physiol Mol Biol Plants

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Abstracts Upland cotton (Gossypium hirsutum L.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as Fusarium oxysporum f. sp. vasinfectum (Fov) causing severe wilt disease. Resistance gene analogs (RGAs) are the largest class of potential resistance (R) genes depicting highly conserved domains and structures in plants. Additionally, RGAs are pivotal components of breeding projects towards host disease resistance, serving as useful functional markers linked to R genes. In this study, a cloning approach based on conserved RGAs motifs was used in order to amplify 38 RGAs from two upland cotton cultivars differing in their Fov susceptibility. Besides, we assessed the phylogenetic expansion and the evolutionary pressures acting upon 127 RGA homologues, which were previously deposited in GenBank along with the 38 RGAs from this study. A total of 165 RGAs sequences were clustered according to their BLAST(P) similarities in ten paralogous genes groups (PGGs). These RGAs exhibited intensive signs of positive selection as it was revealed by inferring various maximum likelihood analyses. The results showed robust signs of positive selection, acting in almost all PGGs across the phylogeny. The evolutionary analysis revealed the existence of 42 positively selected residue sites across the PGG lineages, putatively affecting their ligand-binding specificities. As RGAs derived markers are in close linkage to R genes, these results could be used in ongoing breeding programs of upland cotton.

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Pivoting the Plant Immune System from Dissection to Deployment

Cited by 1,056 publications

References 93 publications

Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing

Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing

uORF-mediated translation allows engineered plant disease resistance without fitness costs

Identification and evidence of positive selection upon resistance gene analogs in cotton (Gossypium hirsutum L.)

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