PIXE and metal hyperaccumulation: from soil to plants and insects

Mesjasz‐Przybyłowicz, Jolanta; Przybyłowicz, W.J.

doi:10.1002/xrs.1304

Cited by 32 publications

(19 citation statements)

References 35 publications

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“…Quantitative studies of concentration and distribution of elements from Na to U are possible on the basis of their K, L and M series of X‐rays, with detection sensitivity down to μg g −1 . Such a broad elemental range enables quantification of macro‐ and micronutrients, for example P, S, K, Ca, Mn, Fe, Cu and Zn, and their correlation with hyperaccumulated elements (Mesjasz‐Przybyłowicz & Przybyłowicz, , ). Simultaneous use of at least one additional analytical technique is a common practice and the facility is referred to as a nuclear microprobe or a proton microprobe.…”

Section: X‐ray Elemental Mapping Techniquesmentioning

confidence: 99%

“…(). Overviews of micro‐PIXE applications in plant sciences are available (Przybyłowicz et al ., ; Mesjasz‐Przybyłowicz & Przybyłowicz, ), as well as a specific review of micro‐PIXE applied to metal hyperaccumulation (Mesjasz‐Przybyłowicz & Przybyłowicz, ). Several recent reviews have provided an explanatory approach of the main synchrotron techniques and their applications in the plant sciences (e.g.…”

Section: Introductionmentioning

confidence: 99%

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X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

et al. 2017

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Contents Summary432I.Introduction433II.Preparation of plant samples for X‐ray micro‐analysis433III.X‐ray elemental mapping techniques438IV.X‐ray data analysis442V.Case studies443VI.Conclusions446Acknowledgements449Author contributions449References449 Summary Hyperaccumulators are attractive models for studying metal(loid) homeostasis, and probing the spatial distribution and coordination chemistry of metal(loid)s in their tissues is important for advancing our understanding of their ecophysiology. X‐ray elemental mapping techniques are unique in providing in situ information, and with appropriate sample preparation offer results true to biological conditions of the living plant. The common platform of these techniques is a reliance on characteristic X‐rays of elements present in a sample, excited either by electrons (scanning/transmission electron microscopy), protons (proton‐induced X‐ray emission) or X‐rays (X‐ray fluorescence microscopy). Elucidating the cellular and tissue‐level distribution of metal(loid)s is inherently challenging and accurate X‐ray analysis places strict demands on sample collection, preparation and analytical conditions, to avoid elemental redistribution, chemical modification or ultrastructural alterations. We compare the merits and limitations of the individual techniques, and focus on the optimal field of applications for inferring ecophysiological processes in hyperaccumulator plants. X‐ray elemental mapping techniques can play a key role in answering questions at every level of metal(loid) homeostasis in plants, from the rhizosphere interface, to uptake pathways in the roots and shoots. Further improvements in technological capabilities offer exciting perspectives for the study of hyperaccumulator plants into the future.

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Section: X‐ray Elemental Mapping Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

et al. 2017

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“…A distinctly different Ni accumulation pattern has been found in Berkheya coddii (Asteraceae) from South Africa (Mesjasz‐Przybylowicz et al ., ; Mesjasz‐Przybyłowicz & Przybyłowicz, , ; Budka et al ., ). In this species, Ni is strongly enriched in the leaf veins and mesophyll, while the concentrations in the epidermis are lower than the average value for the whole leaf (Mesjasz‐Przybyłowicz & Przybyłowicz, ).…”

Section: Introductionmentioning

confidence: 99%

Extreme nickel hyperaccumulation in the vascular tracts of the tree Phyllanthus balgooyi from Borneo

et al. 2015

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SummaryPhyllanthus balgooyi (Phyllanthaceae), one of > 20 nickel (Ni) hyperaccumulator plant species known in Sabah (Malaysia) on the island of Borneo, is remarkable because it contains > 16 wt% Ni in its phloem sap, the second highest concentration of Ni in any living material in the world (after Pycnandra acuminata (Sapotaceae) from New Caledonia with 25 wt% Ni in latex).This study focused on the tissue-level distribution of Ni and other elements in the leaves, petioles and stem of P. balgooyi using nuclear microprobe imaging (micro-PIXE).The results show that in the stems and petioles of P. balgooyi Ni concentrations were very high in the phloem, while in the leaves there was significant enrichment of this element in the major vascular bundles. In the leaves, cobalt (Co) was codistributed with Ni, while the distribution of manganese (Mn) was different. The highest enrichment of calcium (Ca) in the stems was in the periderm, the epidermis and subepidermis of the petiole, and in the palisade mesophyll of the leaf.Preferential accumulation of Ni in the vascular tracts suggests that Ni is present in a metabolically active form. The elemental distribution of P. balgooyi differs from those of many other Ni hyperaccumulator plant species from around the world where Ni is preferentially accumulated in leaf epidermal cells.

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“…By contrast, the bioconcentration factor (BCF), translocation factor (TF) and hyperaccumulation criteria used to evaluate the metal uptake in terrestrial plants have been widely reviewed in recent years [23][24][25][26]. In aquatic plants, heavy metal uptake has been assessed using in some cases, similar factors to those utilized in terrestrial plants.…”

Section: Metal Uptake In Phytofiltration and Phycoremediationmentioning

confidence: 96%

Heavy metal removal in phytofiltration and phycoremediation: the need to differentiate between bioadsorption and bioaccumulation

2012

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Phytoremediation and phycoremediation are cost-effective and environmentally sound technologies for the treatment of polluted streams and wastewaters contaminated with metals. Currently, the most commonly used parameter to assess the metal uptake of biomass is (q) expressed as mg metal g dry weight(-1). By contrast, the bioconcentration factor (BCF) is one of the most widely used factors to evaluate the metal uptake capacity of macrophytes. However, both parameters the metal uptake (q) and the BCF cannot be applied to differentiate between the ability of live plants or photosynthetic microorganisms to adsorb the metal onto their surface through passive mechanisms or to accumulate the contaminant at intracellular level through metabolically active mechanisms. This mini review has the objective of discussing the need to differentiate between bioadsorption and bioaccumulation of metals in live plants and photosynthetic microorganisms used in phytofiltration and phycoremediation processes, respectively. The use of two specific factors, the bioadsorption factor (BAF) and the intracellular accumulation factor (IAF) that have been previously reported in order to make a clear differentiation between these two metal removal mechanisms in Salvinia minima and Leptolyngbya crossbyana is highlighted. It is suggested that the BAF and the IAF can be used in phytofiltration wetlands and phycoremediation lagoons, where there is the need of specific information indicating the fate of the metal in order to gain information about possible removal mechanisms. These factors could also provide a tool to decide whether it is possible to harvest the biomass and to recover a fair amount of metal adsorbed onto the surface by means of desorbent agents. A critical assessment of the use of EDTA as desorbent agent is also included.

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PIXE and metal hyperaccumulation: from soil to plants and insects

Cited by 32 publications

References 35 publications

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

Extreme nickel hyperaccumulation in the vascular tracts of the tree Phyllanthus balgooyi from Borneo

Heavy metal removal in phytofiltration and phycoremediation: the need to differentiate between bioadsorption and bioaccumulation

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