Simultaneous determination of elements in soft tissues of the Japanese oyster Crassostrea gigas was carried out. The recently developed method of PIXE was used in this study. The elements determined repeatedly during the experimental period were Cl, S, K, Zn, Ca, Fe, Br, Cu, Sr, Mn, Ni, Se, Ga, Cr and Ti. These can be classified into five groups in the order of elemental quantity. Localities of several elements, such as K, Ca, Mn, Fe, Cu and Zn among five different tissues such as mantle, gill, digestive diverticula, adductor muscle and remainders were compared in three physiological stages of the oyster. The element K showed no significant difference in five kinds of tissue. A typical accumulation was found for Ca in the mantle. A typical pattern was also observed for Mn in the remainders. The patterns were quite similar for Fe, Cu and Zn. The concentration of Mn, Fe, Cu and Zn in the remainders seems to be decreased as a result of an increase in the remainders weight, while the diminution was not observed for K and Ca. These results suggest that the high accumulation occurs as a result of characteristic affinities of elements with specific tissues and organs due to the physiological activity of the oyster, especially for certain stages, concerning glycogen stores and sexual maturation.