PL-7 Mechanism of foreign RNA recognition in the cytoplasm

Takahasi, Kiyohiro; Yoneyama, Mitsutoshi; Nishihori, Tatsuya; Hirai, Reiko; Kumeta, Hiroyuki; Narita, Ryo; Inagaki, Fuyuhiko; Fujita, Takashi

doi:10.1016/j.cyto.2008.07.029

Cited by 3 publications

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“…The 5′-triphosphate of RNA plays a role in the recognition of pathogenic RNA genomes by RIG-I. 25 We confirmed that calf intestinal alkaline phosphatase (CIAP)-treated IVT-B2 RNA lost the capability of inducing RIG-I/MAVS-related downstream Noxa and TRAIL expression (Supplementary Figure S7a) and IFN-β secretion in PC3 cells (Supplementary Figure S7b). These results suggested that both the double-stranded stem region and 5′-triphosphate of IVT-B2 are crucial in inducing the RIG-I/ MAVS-related downstream anticancer effects.…”

Section: Ivt-di Rna-induced Cancer Cell Death and The Expression Of Apoptosis-related Proteins In Pc3 Cellssupporting

confidence: 55%

An RNA Molecule Derived From Sendai Virus DI Particles Induces Antitumor Immunity and Cancer Cell-selective Apoptosis

2016

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Inactivated Sendai virus (hemagglutinating virus of Japan; HVJ) envelope (HVJ-E) induces anticancer immunity and cancer cell-selective apoptosis through the recognition of viral RNA genome fragments by retinoic acid-inducible gene-I (RIG-I). Here, we discovered that the "copy-back" type of defective-interfering (DI) particles that exist in the Cantell strain of HVJ induced the human PC3 prostate cancer cell death more effectively than the Sendai/52 strain or Cantell strain, which contain fewer DI particles. DI particle genomic RNA (~550 bases) activated proapoptotic genes such as Noxa and/or TNF-related apoptosis-inducing ligand (TRAIL) in human prostate cancer cells to induce cancer cell-selective apoptosis. DI particle-derived RNA was synthesized by in vitro transcription (in vitro transcribed (IVT)-B2). IVT-B2 RNA, which has a double-stranded region in its secondary structure, promoted a stronger anticancer effect than IVT-HN RNA, which does not have a double-stranded region in its secondary structure. The intratumoral transfection of IVT-B2 significantly reduced the volume of a human prostate tumor and induced tumor cell apoptosis in the xenograft mouse model. Moreover, the involvement of natural killer (NK) cells in IVT-B2-RNA-induced anticancer effects was also suggested. These findings provide a novel nucleic acid medicine for the treatment of cancer.

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Section: Ivt-di Rna-induced Cancer Cell Death and The Expression Of Apoptosis-related Proteins In Pc3 Cellssupporting

confidence: 55%

An RNA Molecule Derived From Sendai Virus DI Particles Induces Antitumor Immunity and Cancer Cell-selective Apoptosis

2016

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“…The C-terminal domain (CTD) consists of four conserved cysteines (C810, C813, C864, C869) linked by zinc or, less commonly, a mercury atom. These are the major structural and functional components of this protein that is responsible for transmitting the signal that activates the antiviral response by binding dsRNA and ssRNA 5'-triphosphate (23,24). The activation of RIG-I receptor expression is only possible when the appropriate ligand interacts with the CTD domain (9).…”

Section: Rig-i-like Receptorsmentioning

confidence: 99%

Factors affecting RIG-I-Like receptors activation - New research direction for viral hemorrhagic fevers

Małkowska

Niedźwiedzka-Rystwej

2022

Front. Immunol.

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Viral hemorrhagic fever (VHF) is a term referring to a group of life-threatening infections caused by several virus families (Arenaviridae, Bunyaviridae, Filoviridae and Flaviviridae). Depending on the virus, the infection can be mild and can be also characterized by an acute course with fever accompanied by hypervolemia and coagulopathy, resulting in bleeding and shock. It has been suggested that the course of the disease is strongly influenced by the activation of signaling pathways leading to RIG-I-like receptor-dependent interferon production. RIG-I-like receptors (RLRs) are one of two major receptor families that detect viral nucleic acid. RLR receptor activation is influenced by a number of factors that may have a key role in the differences that occur during the antiviral immune response in VHF. In the present study, we collected data on RLR receptors in viral hemorrhagic fevers and described factors that may influence the activation of the antiviral response. RLR receptors seem to be a good target for VHF research, which may contribute to better therapeutic and diagnostic strategies. However, due to the difficulty of conducting such studies in humans, we suggest using Lagovirus europaeus as an animal model for VHF.

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“…Retinoic acid-induced gene I (RIG-I) is a cytosolic receptor that senses double-stranded RNA (dsRNA) originating from pathogens such as viruses (2)(3)(4)(5). Binding to dsRNA disrupts an intramolecular interaction that keeps RIG-I in an autoinhibitory state (6,7), triggering an overall conformational change that releases the N-terminal CARD domain (8, 9). The N-terminal CARD of RIG-I undergoes homotypic oligomerization and heterooligomerization with that of the mitochondrion antiviral signaling (MAVS) adaptor molecule (10).…”

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confidence: 99%

Emerging Roles of Protein Deamidation in Innate Immune Signaling

Zhao

et al. 2016

J Virol

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Protein deamidation has been considered a nonenzymatic process associated with protein functional decay or "aging." Recent studies implicate protein deamidation in regulating signal transduction in fundamental biological processes, such as innate immune responses. Work investigating gammaherpesviruses and bacterial pathogens indicates that microbial pathogens deploy deamidases or enzyme-deficient homologues (pseudoenzymes) to induce deamidation of key signaling components and evade host immune responses. Here, we review studies on protein deamidation in innate immune signaling and present several imminent questions concerning the roles of protein deamidation in infection and immunity.

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PL-7 Mechanism of foreign RNA recognition in the cytoplasm

Cited by 3 publications

References 0 publications

An RNA Molecule Derived From Sendai Virus DI Particles Induces Antitumor Immunity and Cancer Cell-selective Apoptosis

An RNA Molecule Derived From Sendai Virus DI Particles Induces Antitumor Immunity and Cancer Cell-selective Apoptosis

Factors affecting RIG-I-Like receptors activation - New research direction for viral hemorrhagic fevers

Emerging Roles of Protein Deamidation in Innate Immune Signaling

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