Place of salivary β glucuronidase activity in head &amp; neck cancers

Ghadge, M. S.; Raste, A. S.

doi:10.1007/bf02913096

Cited by 1 publication

(2 citation statements)

References 6 publications

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“…Several publications have shown that saliva contains betaglucuronidase enzymes [35][36][37]. To investigate whether these OF enzymes are able to hydrolyze cannabinoid conjugates, the degree of degradation of two glucuronides was evaluated under various conditions to discriminate between nonenzymatic (chemical) and enzymatic hydrolysis.…”

Section: Chemical and Enzymatic Hydrolysis Of Glucuronides In Ofmentioning

confidence: 99%

“…In the latter case, the presence of beta-glucuronidase enzymes in OF [35][36][37] could explain the lack of detection of THCCOOH-gluc and THC-gluc in OF. In agreement with the assumption that glucuronide conjugates of cannabinoids are present in OF in significant amounts, a study published in 2007 by Moore et al [4] suggests that 48.2 % of THCCOOH is glucuronidated in OF (estimated after beta-glucuronidase treatment).…”

Section: Analyses Of Authentic Oral Fluid Samplesmentioning

confidence: 99%

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Analysis of cannabinoids in oral fluid by liquid chromatography–tandem mass spectrometry

et al. 2012

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A sensitive method was developed for quantifying a wide range of cannabinoids in oral fluid (OF) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). These cannabinoids include D 9-tetrahydrocannabinol (THC), 11-hydroxy-D 9-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-D 9-tetrahydrocannabinol (THCCOOH), cannabinol (CBN), cannabidiol (CBD), D 9-tetrahydrocannabinolic acid A (THC-A), 11-nor-9-carboxy-D 9-tetrahydrocannabinol glucuronide (THCCOOH-gluc), and D 9-tetrahydrocannabinol glucuronide (THC-gluc). Samples were collected using a Quantisal TM device. The advantages of performing a liquidliquid extraction (LLE) of KCl-saturated OF using heptane/ ethyl acetate versus a solid-phase extraction (SPE) using HLB copolymer columns were determined. Chromatographic separation was achieved in 11.5 min on a Kinetex TM column packed with 2.6-lm core-shell particles. Both positive (THC, 11-OH-THC, CBN, and CBD) and negative (THCCOOH, THC-gluc, THCCOOH-gluc, and THC-A) electrospray ionization modes were employed with multiple reaction monitoring using a high-end AB Sciex API 5000 TM triple quadrupole LC-MS/MS system. Unlike SPE, LLE failed to extract THC-gluc and THCCOOH-gluc. However, the LLE method was more sensitive for the detection of THCCOOH than the SPE method, wherein the limit of detection (LOD) and limit of quantification (LOQ) decreased from 100 to 50 pg/ml and from 500 to 80 pg/ml, respectively. The two extraction methods were successfully applied to OF samples collected from volunteers before and after they smoked a homemade cannabis joint. High levels of THC were measured soon after smoking, in addition to significant amounts of THC-A. Other cannabinoids were found in low concentrations. Glucuronide conjugate levels were lower than the method's LOD for most samples. Incubation studies suggest that glucuronides could be enzymatically degraded by glucuronidase prior to OF collection.

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Section: Chemical and Enzymatic Hydrolysis Of Glucuronides In Ofmentioning

confidence: 99%