Placenta extract promote liver regeneration in CCl4-injured liver rat model

Jung, Jieun; Lee, Hyunjung; Lee, Jung Min; Na, Kyu-Hwan; Hwang, Seong-Gyu; Kim, Gi Jin

doi:10.1016/j.intimp.2011.02.012

Cited by 48 publications

(47 citation statements)

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“…There are a number of methods, such as placenta extract [40], platelet [41], and carbon monoxide [42], have been shown to promote LR in animal studies. However, none of these methods have been translated into clinical practice because of their low safety and efficacy.…”

Section: Discussionmentioning

confidence: 99%

Down-Regulation of microRNA-26a Promotes Mouse Hepatocyte Proliferation during Liver Regeneration

Zhou

Wang

et al. 2012

PLoS ONE

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BackgroundInadequate liver regeneration (LR) is still an unsolved problem in major liver resection and small-for-size syndrome post-living donor liver transplantation. A number of microRNAs have been shown to play important roles in cell proliferation. Herein, we investigated the role of miR-26a as a pivotal regulator of hepatocyte proliferation in LR.Methodology/Principal FindingsAdult male C57BL/6J mice, undergoing 70% partial hepatectomy (PH), were treated with Ad5-anti-miR-26a-LUC or Ad5-miR-26a-LUC or Ad5-LUC vector via portal vein. The animals were subjected to in vivo bioluminescence imaging. Serum and liver samples were collected to test liver function, calculate liver-to-body weight ratio (LBWR), document hepatocyte proliferation (Ki-67 staining), and investigate potential targeted gene expression of miR-26a by quantitative real-time PCR and Western blot. The miR-26a level declined during LR after 70% PH. Down-regulation of miR-26a by anti-miR-26a expression led to enhanced proliferation of hepatocytes, and both LBWR and hepatocyte proliferation (Ki-67+ cells %) showed an increased tendency, while liver damage, indicated by aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin (T-Bil), was reduced. Furthermore, CCND2 and CCNE2, as possible targeted genes of miR-26a, were up-regulated. In addition, miR-26a over-expression showed converse results.Conclusions/SignificanceMiR-26a plays crucial role in regulating the proliferative phase of LR, probably by repressing expressions of cell cycle proteins CCND2 and CCNE2. The current study reveals a novel miRNA-mediated regulation pattern during the proliferative phase of LR.

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Section: Discussionmentioning

confidence: 99%

Down-Regulation of microRNA-26a Promotes Mouse Hepatocyte Proliferation during Liver Regeneration

Zhou

Wang

et al. 2012

PLoS ONE

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“…5 HPE has been approved by the FDA for use in humans, and is now being used widely for treatment of tiredness. 6,7 Studies using animal models have provided evidence that HPE has liver function improvement 8 and wound healing 9 attributes, as assessed via liver regeneration and production of transforming growth factor-b and vascular endothelial growth factor, respectively. However, despite the recent popularity of HPE, its mechanism is not yet fully understood.…”

Section: Introductionmentioning

confidence: 99%

Potential synergistic effects of human placental extract and minoxidil on hair growth-promoting activity in C57BL/6J mice

Kwon

Park

et al. 2015

Clin Exp Dermatol

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“…Jung and co-workers [138] recently showed that placenta extracts may be used to promote liver regeneration in CCl 4 -injured rats. The authors investigated the use of extracts both in vitro (on rat hepatic cells) and in vivo.…”

Section: Placenta-derived Stem Cellsmentioning

confidence: 99%

Recent Advances in Derivation of Functional Hepatocytes from Placental Stem Cells

Iacono

Anzalone²,

Corrao

et al. 2013

TOTERMJ

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End-stage liver diseases are one of the leading causes of death in the world. Often orthotopic liver transplantation represents the final therapeutic choice. The limits of this approach are the scarcity of donor livers available, and the many side effects related to the administration of immune suppressants to the patients. Cellular therapy for liver diseases is increasingly being viewed as a promising strategy to provide hepatocytes to replenish the parenchymal cells of the organ. This technique suffers of some important limitations, such as the difficulty in isolating sufficient cell numbers (e.g. when adult or foetal hepatocytes are used for transplantation), the limited viability of isolated hepatocytes and, when applicable, the limited differentiation of stem cells (when hepatocyte-like cells are derived from hepatic or extra-hepatic progenitor populations).In recent years, perinatal stem cells have been proposed as reliable cellular populations which may be successfully used to derive hepatocyte-like cells. These cells feature key advantages over other adult stem cells: may be easily sourced from the tissues of origin, can be expanded ex vivo to obtain high cell numbers, may be differentiated towards hepatocyte-like cells. In addition, these cells feature relevant immunomodulatory and anti-inflammatory activities, and their sourcing is not limited by ethical concernsIn the present review we analyze the molecular basis of hepatocyte biology and development, and discuss the recent advances in deriving hapatocyte-like cells from perinatal stem cells. Very recent papers on mesenchymal stem cells derived from bone marrow and adipose tissues are also comparatively discussed as prototypes of the use of adult extrahepatic stem cells. In our opinion, perinatal stem cells do represent a promising tool for liver regenerative medicine, and recent research reports further strengthened this perception and fostered further efforts by multiple research groups worldwide.

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Placenta extract promote liver regeneration in CCl4-injured liver rat model

Cited by 48 publications

References 50 publications

Down-Regulation of microRNA-26a Promotes Mouse Hepatocyte Proliferation during Liver Regeneration

Down-Regulation of microRNA-26a Promotes Mouse Hepatocyte Proliferation during Liver Regeneration

Potential synergistic effects of human placental extract and minoxidil on hair growth-promoting activity in C57BL/6J mice

Recent Advances in Derivation of Functional Hepatocytes from Placental Stem Cells

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