Placental miRNAs in feto-maternal communication mediated by extracellular vesicles

Morales-Prieto, Diana M.; Favaro, Rodolfo R.; Markert, Udo R.

doi:10.1016/j.placenta.2020.07.001

“…The placenta separates maternal and fetal circulation. miRNAs may facilitate placental communication with the maternal and fetal compartments, reviewed in [ 34 ]. An in silico comparison between human placental enriched genes ( n = 494) derived from antibody-based protein profiling of healthy placenta tissue and all uniquely predicted targets ( n = 3322) of the eight selected miRNAs demonstrated an overlap of 84 genes (hypergeometric overlap statistics p < 10 −30 , Supplementary Figure S3 ).…”

Section: Resultsmentioning

confidence: 99%

The Temporal Profile of Circulating miRNAs during Gestation in Overweight and Obese Women with or without Gestational Diabetes Mellitus

Sørensen

¹

,

Poppel

²

,

Desoyé

³

et al. 2022

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Circulating non-coding microRNAs (miRNAs) are important for placentation, but their expression profiles across gestation in pregnancies, which are complicated by gestational diabetes mellitus (GDM), have not been fully established. Investigating a single time point is insufficient, as pregnancy is dynamic, involving several processes, including placenta development, trophoblast proliferation and differentiation and oxygen sensing. Thus, the aim of this study was to compare the temporal expression of serum miRNAs in pregnant women with and without GDM. This is a nested case-control study of longitudinal data obtained from a multicentric European study (the ‘DALI’ study). All women (n = 82) were overweight/obese (BMI ≥ 29 kg/m2) and were normal glucose tolerant (NGT) at baseline (before 20 weeks of gestation). We selected women (n = 41) who were diagnosed with GDM at 24–28 weeks, according to the IADPSG/WHO2013 criteria. They were matched with 41 women who remained NGT in their pregnancy. miRNA (miR-16-5p, -29a-3p, -103-3p, -134-5p, -122-5p, -223-3p, -330-3p and miR-433-3p) were selected based on their suggested importance for placentation, and measurements were performed at baseline and at 24–28 and 35–37 weeks of gestation. Women with GDM presented with overall miRNA levels above those observed for women remaining NGT. In both groups, levels of miR-29a-3p and miR-134-5p increased consistently with progressing gestation. The change over time only differed for miR-29a-3p when comparing women with GDM with those remaining NGT (p = 0.044). Our findings indicate that among overweight/obese women who later develop GDM, miRNA levels are already elevated early in pregnancy and remain above those of women who remain NGT during their pregnancy. Maternal circulating miRNAs may provide further insight into placentation and the cross talk between the maternal and fetal compartments.

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“…Placental miRNAs are temporally expressed throughout gestation and have critical roles in trophoblast differentiation and function 34,35 , thus the miRNAome was pro led to assess alterations in expression relative to infection. miRNA-seq identi ed expression of 326 miRNAs in TSCs, 335 miRNAs in ST3Ds, and 317 miRNAs in EVTs.…”

Section: Resultsmentioning

confidence: 99%

Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts

Block

¹

,

Schmidt

²

,

McKeon

³

et al. 2021

Preprint

0

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Zika virus (ZIKV) infection at the maternal-placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into early gestation syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT). TSCs and STs, but not EVTs, were highly permissive to productive infection with ZIKV strain DAK AR 41524. The impact of ZIKV on the cellular transcriptome showed that infection of TSCs and STs increased expression of immune related genes, including those involved in type I and type III interferon responses. ZIKV exposure altered extracellular vesicle (EV) protein, mRNA, and miRNA cargo, regardless of productive infection. These findings suggest that early gestation macaque TSCs and STs are permissive to ZIKV infection, and that EV analysis may provide a foundation for identifying non-invasive biomarkers of placental infection in a highly translational model.

show abstract

“…Placental miRNAs are temporally expressed throughout gestation and have critical roles in trophoblast differentiation and function 34,35 , thus the miRNAome was profiled to assess alterations in expression relative to infection. miRNA-seq identified expression of 326 miRNAs in TSCs, 335 miRNAs in ST3Ds, and 317 miRNAs in EVTs.…”

Section: Resultsmentioning

confidence: 99%

Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts

Block

¹

,

Schmidt

²

,

McKeon

³

et al. 2021

Preprint

1

0

View full text Add to dashboard Cite

Zika virus (ZIKV) infection at the maternal-placental interface is associated with adverse pregnancy outcomes including fetal demise and pregnancy loss. To determine how infection impacts placental trophoblasts, we utilized rhesus macaque trophoblast stem cells (TSC) that can be differentiated into early gestation syncytiotrophoblasts (ST) and extravillous trophoblasts (EVT). TSCs and STs, but not EVTs, were highly permissive to productive infection with ZIKV strain DAK AR 41524. The impact of ZIKV on the cellular transcriptome showed that infection of TSCs and STs increased expression of immune related genes, including those involved in type I and type III interferon responses. ZIKV exposure altered extracellular vesicle (EV) protein, mRNA, and miRNA cargo, regardless of productive infection. These findings suggest that early gestation macaque TSCs and STs are permissive to ZIKV infection, and that EV analysis may provide a foundation for identifying non-invasive biomarkers of placental infection in a highly translational model.

show abstract

Placental miRNAs in feto-maternal communication mediated by extracellular vesicles

Cited by 41 publications

References 65 publications

The Temporal Profile of Circulating miRNAs during Gestation in Overweight and Obese Women with or without Gestational Diabetes Mellitus

The Temporal Profile of Circulating miRNAs during Gestation in Overweight and Obese Women with or without Gestational Diabetes Mellitus

Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts

Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts

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