Placental neutrophil count as an early predictor in neonatal sepsis

Abstract

This present study aims to assess the Placenta Neutrophil Count (PNC) as an early marker in the diagnosis of Neonatal Sepsis (NS). This study was conducted in February-May 2017. Cord blood samples and placental tissues were taken from 15 each of newborns at risk of sepsis (case group) and without risk of sepsis (control group). The PNC was estimated using conventional light microscope. We also measured some oxidative stress parameters from the cord blood samples, i.e., Hydrogen Peroxide (H 2 O 2 ), thiocyanate (SCN), myeloperoxidase (MPO) enzyme and Advance Oxidation Protein Products (AOPPs) to investigate the role of neutrophil in placenta. The results show that the PNC, the level of AOPPs and SCN, and MPO activity of cord blood were higher in the case group than the control group, while for the H 2 O 2 level show the opposite result.From this results, it can be assumed thatneutrophil have a role in NS and might be use as a potential marker for early prediction of NS. These indings need to be veri ied by further investigation of fresh samples, in a larger, prospective cohort, and with sophisticated analysis evidenced by deep sequencing. The placental pathologic and histologic examination has been used as a diagnostic tool for neonatologist in many neonatal diseases, except NS. This result suggested that PNC might can be used as an early predictor for NS.