Placental proteome alterations in women with intrahepatic cholestasis of pregnancy

He, Pei; Wang, Furong; Jiang, Yan; Zhong, Yingying; Lan, Yongfei; Chen, Shuqing

doi:10.1016/j.ijgo.2014.03.035

Cited by 14 publications

(5 citation statements)

References 23 publications

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“…In our study, more proteins in the placental tissue were identified with higher resolution mass spectrometry, which is 3337, more DEPs including 37 upregulated and 141 downregulated proteins were found. Compared with He et al [ 14 ] study, only 37 DEPs were identified in the placental tissue of ICP patients compared with normal controls by the method of two-dimensional electrophoresis and mass spectrometry. In Zhang et al study [ 15 ], only 2120 proteins were identified in the placental tissue of ICP patients compared with normal controls by isobaric tags for relative and absolute quantification-based proteomics approach, including 29 upregulated proteins and 9 downregulated proteins.…”

Section: Discussionmentioning

confidence: 68%

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy

Zeng,

Hou,

et al. 2024

Reprod. Sci.

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Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease, which can lead to adverse fetal outcomes, including preterm labor and intrauterine death. The pathogenesis of ICP is still unclear. We hypothesized that pathological index leads to abnormal placenta changes in ICP. Investigation of these differences in protein expression in parallel profiling is essential to understand the comprehensive pathophysiological mechanism underlying ICP. The present study screened differentially expressed proteins (DEPs) as novel diagnostic markers for ICP. Proteomic profiles of placental tissues from 32 ICP patients and 24 healthy volunteers (controls) were analyzed. Our founding was valid by following western blotting and immunohistochemistry staining, respectively. The association of the key protein expression with clinicopathological features of ICP was further analyzed. A total of 178 DEPs were identified between the ICP and control groups. Functional enrichment analysis showed these proteins were significantly enriched in the PPAR singling pathway by KEGG and PPARα/RXRα activation by IPA. Apolipoprotein A2 (APOA2) was the only upregulated protein, which uniquely identified in ICP groups and related to both pathways. Validation of western blotting and immunohistochemical staining analysis showed significantly higher APOA2 expression in the ICP group than in the control group. Furthermore, the expression of APOA2 is associated with clinicopathological features in ICP groups. Receiver operating characteristic (ROC) curve analyses showed that the AUC of APOA2 was 0.8984 (95% confidence interval (CI): 0.772–1.000). This study has identified up-regulated APOA2 associated with PPAR singling pathway and PPARα/RXRα activation in ICP. Thus, APOA2 may be involved in ICP pathogenesis, serving as a novel biomarker for its diagnosis.

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Section: Discussionmentioning

confidence: 68%

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy

Zeng,

Hou,

et al. 2024

Reprod. Sci.

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“…Thus, mass spectrometry based proteomics can be used to study the expression levels, isoforms and post-translational modifications of proteins. The present review collected a total of 9 proteomics studies of ICP (27)(28)(29)(30)(31)(32)(33)(34)(35), including 8 untargeted and 1 targeted studies (Table 2). There are three types of technologies for the analyses of untargeted proteomics studies, including: 2D-PAGE MS (two-dimensional polyacrylamide gel electrophoresis followed by mass spectrometry), DDA (data-dependent acquisition) based LC-MS/MS (Liquid chromatography-tandem mass spectrometry), and DIA (data-independent acquisition) based LC-MS/MS.…”

Section: Proteomics Strategiesmentioning

confidence: 99%

An omics review and perspective of researches on intrahepatic cholestasis of pregnancy

Wang,

Chen,

et al. 2024

Front. Endocrinol.

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Intrahepatic cholestasis of pregnancy (ICP) is one of the common pregnancy complications that may threaten the health of both pregnant women and their fetuses. Hence, it is of vital importance to identify key moleculars and the associated functional pathways of ICP, which will help us to better understand the pathological mechanisms as well as to develop precise clinical biomarkers. The emerging and developing of multiple omics approaches enable comprehensive studies of the genome, transcriptome, proteome and metabolome of clinical samples. The present review collected and summarized the omics based studies of ICP, aiming to provide an overview of the current progress, limitations and future directions. Briefly, these studies covered a broad range of research contents by the comparing of different experimental groups including ICP patients, ICP subtypes, ICP fetuses, ICP models and other complications. Correspondingly, the studied samples contain various types of clinical samples, in vitro cultured tissues, cell lines and the samples from animal models. According to the main research objectives, we further categorized these studies into two groups: pathogenesis and diagnosis analyses. The pathogenesis studies identified tens of functional pathways that may represent the key regulatory events for the occurrence, progression, treatment and fetal effects of ICP. On the other hand, the diagnosis studies tested more than 40 potential models for the early-prediction, diagnosis, grading, prognosis or differential diagnosis of ICP. Apart from these achievements, we also evaluated the limitations of current studies, and emphasized that many aspects of clinical characteristics, sample processing, and analytical method can greatly affect the reliability and repeatability of omics results. Finally, we also pointed out several new directions for the omics based analyses of ICP and other perinatal associated conditions in the future.

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“…Comparative proteomics analysis found less differentially expressed proteins. He et al reported 37 differentially expressed proteins, which involved in cytoskeleton activity, blood coagulation, and platelet activation as well as chaperones, heat shock proteins, RNA-binding and calcium-binding proteins, and various enzymes [ 88 ]. Zhang et al found 38 differentially expressed proteins, among which 29 were upregulated and 9 were downregulated in ICP placenta [ 89 ].…”

Section: The Role Of Bile Acid and Its Receptorsmentioning

confidence: 99%

Molecular Pathogenesis of Intrahepatic Cholestasis of Pregnancy

Xiao

Zhu

Song

et al. 2021

Canadian Journal of Gastroenterology and Hepatology

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Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease. The maternal symptoms are characterized by skin pruritus and elevated bile acids, causing several adverse outcomes for fetuses, including an increased risk of preterm birth, meconium-stained amniotic fluid, neonatal depression, respiratory distress syndrome, and stillbirth. Genetic, hormonal, immunological, and environmental factors contribute to the pathogenesis of ICP, and the estrogen-bile acid axis is thought to play a dominant role. The advances in the past 10 years uncover more details of this axis. Moreover, dysregulation of extracellular matrix and oxygen supply, organelle dysfunction, and epigenetic changes are also found to cause ICP, illuminating more potential drug targets for interfering with. Here, we summarize the molecular pathogenesis of ICP with an emphasis on the advancement in the past 10 years, aiming to give an updated full view of this field.

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Placental proteome alterations in women with intrahepatic cholestasis of pregnancy

Cited by 14 publications

References 23 publications

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy

An omics review and perspective of researches on intrahepatic cholestasis of pregnancy

Molecular Pathogenesis of Intrahepatic Cholestasis of Pregnancy

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