Placental tumor necrosis factor-<b>α</b> protein expression during normal human gestation

Basu, Jayasri; Agamasu, Enyonam; Bendek, Bolek; Salafia, Carolyn M.; Mishra, Abha; Benfield, Nerys; Prasad, Priya; Mikhail, Magdy S.

doi:10.3109/14767058.2016.1156668

Cited by 14 publications

(16 citation statements)

References 29 publications

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“…EVTs were reported to stimulate VSMC apoptosis (Harris et al, ) and migration (Bulmer et al, ) during spiral artery remodeling. In addition, TNF‐α (Basu et al, ), FasL (Harris et al, ), and TRAIL (Keogh et al, ) secreted by EVTs were the key factors mediating this process. In this study, we confirmed that the expression of TNF‐α, FasL, and TRAIL was significantly decreased in HTR‐8/SVneo cells after MEG3 overexpression.…”

Section: Discussionmentioning

confidence: 99%

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

Tao

Liu

et al. 2019

Molecular Reproduction Devel

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Extravillous trophoblasts (EVTs) migrate into uterine decidua and induce vascular smooth muscle cell (VSMC) loss through mechanisms thought to involve migration and apoptosis, achieving complete spiral artery remodeling. Long noncoding RNA maternally expressed gene 3 (MEG3) can regulate diverse cellular processes, such as proliferation and migration, and has been discovered highly expressed in human placenta tissues. However, little is known about the role of MEG3 in modulating EVT functions and EVT‐induced VSMC loss. In this study, we first examined the location of MEG3 in human first‐trimester placenta by in situ hybridization. Then, exogenous upregulation of MEG3 in HTR‐8/SVneo cells was performed to investigate the effects of MEG3 on EVT motility and EVT capacity to displace VSMCs. Meanwhile, the molecules mediating EVT‐induced VSMC loss, such as tumor necrosis factor‐α (TNF‐α), Fas ligand (FasL), and tumor necrosis factor‐α‐related apoptosis‐inducing ligand (TRAIL) were detected at transcriptional and translational levels. Finally, VSMCs were cocultured with MEG3‐upregulated HTR‐8/SVneo to explore the role of MEG3 on EVT‐mediated VSMC migration and apoptosis. Results showed that MEG3 was expressed in trophoblasts in placental villi and decidua, and MEG3 enhancement inhibited HTR‐8/SVneo migration and invasion. Meanwhile, the displacement of VSMCs by HTR‐8/SVneo and the expression of TNF‐α, FasL and TRAIL in HTR‐8/SVneo were reduced following MEG3 overexpression in HTR‐8/SVneo. Furthermore, HTR‐8/SVneo with MEG3 upregulation impaired VSMC migration and apoptosis. The PI3K/Akt pathway, which is possibly downstream, was inactivated in MEG3‐upregulated HTR‐8/SVneo. These findings suggest that MEG3 might be a negative regulator of spiral artery remodeling via suppressing EVT invasion and EVT‐mediated VSMC loss.

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Section: Discussionmentioning

confidence: 99%

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

Tao

Liu

et al. 2019

Molecular Reproduction Devel

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“…7,8 In a previous study we reported that placental TNF-a protein expression in women with uncomplicated pregnancy peaks in the second trimester. 9 In the present study, we simultaneously investigated the patterns of changes in placental villous MMP-9 and TNF-a protein expression to determine the correlation between these 2 proteins throughout normal human gestation.…”

Section: Introductionmentioning

confidence: 99%

Correlation Between Placental Matrix Metalloproteinase 9 and Tumor Necrosis Factor-α Protein Expression Throughout Gestation in Normal Human Pregnancy

et al. 2018

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Matrix metalloproteinases (MMPs), specifically MMP-9 plays a role in human placentation. The enzyme confers an invasive ability to cytotrophoblasts and degrades the endometrial matrix as the cells infiltrate the decidua to keep up with placental growth. Since tumor necrosis factor-a (TNF-a) can induce the synthesis of MMP-9, we investigated the patterns of changes in and correlation between placental villous MMP-9 and TNF-a expressions throughout normal human gestation. Placentas were obtained from 179 normal pregnant women who underwent elective abortion or term delivery. Chorionic villi isolated from placental samples were grouped as first, second, and third trimester (7 0/7 -13 0/7 , 13 1/7 -23 6/7 , and 37 0/7 -42 4/7 weeks, respectively). Chorionic villous TNF-a and MMP-9 proteins were assayed using enzyme immunoassay kits. There were significant differences in MMP-9 and TNF-a protein expressions among the trimester groups (P ¼ .001). The MMP-9 protein increased progressively with an increase in gestational age (GA), but TNF-a peaked in the second trimester. Within each trimester group, we searched for the effects of variation of GA in days on the 2 variables. A significant positive correlation between MMP-9 and GA was noted in the first trimester (r ¼ 0.364, P ¼ .005). No other comparisons were significant. When GA was controlled for, partial correlation revealed a significant positive correlation between TNF-a and MMP-9 only in the second trimester (r ¼ 0.300, P ¼ .018). We hypothesize that the TNF-a peak and the positive correlation between TNF-a and MMP-9 in the second trimester of normal human gestation could contribute toward a successful pregnancy outcome.

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“…Tumor necrosis factor‐α is a cytokine that plays a role in the pathophysiology of inflammation and in pregnancy and labor . Etanercept (ETN) is a circulating receptor fusion protein within the class of drugs known as TNF‐α inhibitors (TNFi).…”

Section: Introductionmentioning

confidence: 99%

“…Tumor necrosis factor-α is a cytokine that plays a role in the pathophysiology of inflammation and in pregnancy and labor. 1,2 Etanercept (ETN) is a circulating receptor fusion protein within the class of drugs known as TNF-α inhibitors (TNFi). By inhibiting inflammatory processes, ETN and other TNFis have been found effective for treatment of inflammatory diseases, including chronic inflammatory arthritis (cIA) and psoriasis (PsO).…”

Section: Introductionmentioning

confidence: 99%

Pregnancy and infant outcomes including major congenital malformations among women with chronic inflammatory arthritis or psoriasis, with and without etanercept use

Carman

Accortt

Anthony

et al. 2017

Pharmacoepidemiology and Drug

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Placental tumor necrosis factor-α protein expression during normal human gestation

Cited by 14 publications

References 29 publications

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

LncRNA MEG3 inhibits trophoblast invasion and trophoblast‐mediated VSMC loss in uterine spiral artery remodeling

Correlation Between Placental Matrix Metalloproteinase 9 and Tumor Necrosis Factor-α Protein Expression Throughout Gestation in Normal Human Pregnancy

Pregnancy and infant outcomes including major congenital malformations among women with chronic inflammatory arthritis or psoriasis, with and without etanercept use

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