Planar chromatography – Current practice and future prospects

Wilson, Ian D.; Poole, Colin F.

doi:10.1016/j.jchromb.2022.123553

Cited by 17 publications

(14 citation statements)

References 162 publications

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“…For water samples requiring a high concentration factor octadecylsiloxane-bonded silica and (hypercrosslinked) porous organic polymer sorbents are typically used [3,4,86]. Solid phase microextraction techniques are not well represented among HPTLC methods although the scale would be appropriate for streamlined sample processing.…”

Section: Solid-phase Extractionmentioning

confidence: 99%

“…Also, the whole bioactivity of the sample can be observed on the layer in contrast to column-based methods where only fractions eluted from the column are accessible for bioassay. For screening environmental (and other) extracts common bioassays include bioluminescence bacteria for general toxicity assessment, enzyme inhibition assays for neurotoxins, algal and fungi spore assays for herbicides and fungicides, genetically engineered microorganisms for genotoxins, and genetically modified yeast cells for hormonal toxins [3,4,[9][10][11][96][97][98][99][100]. Identification of unknown compounds is typically by in situ extraction combined with ESI-MS [3,4,8,101].…”

Section: Solid-phase Extractionmentioning

confidence: 99%

“…HPTLC requires a considerable capital investment to support state-of-theart separations and analysts who use conventional TLC practices have not been sufficiently vocal or interested in advocating for suitable facilities to sperm major growth in HPTLC. This is unfortunate as HPTLC has much to offer as a complementary technique to high-performance liquid chromatography (HPLC) [4]. Growing interest outside of the TLC community is being driven by a higher level of automation with keyboard control of experimental variables [3,5], direct coupling to MS detection for structure assignment and quantification [3][4][5][6][7], and effects-directed detection employing chemical, enzymatic, microorganisms, and cell-based assays for bioactivity reporting [3,4,[8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…This is unfortunate as HPTLC has much to offer as a complementary technique to high-performance liquid chromatography (HPLC) [4]. Growing interest outside of the TLC community is being driven by a higher level of automation with keyboard control of experimental variables [3,5], direct coupling to MS detection for structure assignment and quantification [3][4][5][6][7], and effects-directed detection employing chemical, enzymatic, microorganisms, and cell-based assays for bioactivity reporting [3,4,[8][9][10][11]. The last two areas represent a significant influx of new applications for HPTLC highlighted by an increasing number of literature citations.…”

Section: Introductionmentioning

confidence: 99%

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Section: Solid-phase Extractionmentioning

confidence: 99%

Section: Solid-phase Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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“…In this vein, proteomics becomes an indispensable tool for studying carcinogenesis and other pathological processes. [1][2][3][4] Owing to their convenience and efficiency, various separation methods have been introduced for protein enrichment, including high performance liquid chromatography (HPLC), 5 capillary electrophoresis, 6 supercritical CO 2 fractionation technology, [7][8][9][10][11] etc.…”

Section: Introductionmentioning

confidence: 99%

Magnetic resin composites for the enrichment of proteins, peptides and phosphopeptides

Wang¹,

Pan²,

Yan³

et al. 2023

Anal. Methods

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Extraction solvent selection for Cannabis sativa L. by efficient exploration of cannabinoid selectivity and phytochemical diversity

Tzimas,

Petrakis,

Halabalaki

et al. 2023

Phytochemical Analysis

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IntroductionCannabis sativa L. is attracting worldwide attention due to various health‐promoting effects. Extraction solvent type is critical for the recovery of bioactive compounds from the plant, especially cannabinoids. However, the choice of solvent is varied and not adequately warranted elsewhere, causing confusion in involved fields.ObjectiveThe present work aimed to investigate the effect of extraction solvent on C. sativa (hemp) with regard to cannabinoid recovery and phytochemical profile of the extracts, considering most of the related solvents.MethodologyThe majority of solvents reported for C. sativa (n = 14) were compared using a representative hemp pool. Quantitative results for major and minor cannabinoids were rapidly and reliably obtained using ultrahigh‐performance liquid chromatography coupled with photodiode array detection (UPLC‐PDA). In parallel, high‐performance thin‐layer chromatographic (HPTLC) fingerprinting was employed, involving less toxic mobile phase than in relevant reports. Various derivatisation schemes were applied for more comprehensive comparison of extracts.ResultsDifferential selectivity towards cannabinoids was observed among solvents. MeOH was found particularly efficient for most cannabinoids, in addition to solvent systems such as n‐Hex/EtOH 70:30 and ACN/EtOH 80:20, while EtOH was generally inferior. For tetrahydrocannabinol (THC)‐type compounds, EtOAc and n‐Hex/EtOAc 60:40 outperformed n‐Hex, despite its use in the official EU method. Solvents that tend to extract more lipids or more polar compounds were revealed based on HPTLC results.ConclusionCombining the observations from UPLC quantitation and HPTLC fingerprinting, this work allowed comprehensive evaluation of extraction solvents, in view of robust quality assessment and maximised utilisation of C. sativa.

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Planar chromatography – Current practice and future prospects

Cited by 17 publications

References 162 publications

Sample preparation for planar chromatography

Sample preparation for planar chromatography

Magnetic resin composites for the enrichment of proteins, peptides and phosphopeptides

Extraction solvent selection for Cannabis sativa L. by efficient exploration of cannabinoid selectivity and phytochemical diversity

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