Planar chromatography in studies of optically active drugs

Abstract

The use of planar chromatography for the investigation and separation of optically active drugs into individual stereoisomers with subsequent identification of isomers was demonstrated using amino acids and amlodipine besylate as examples.

Pharmaceuticals and Related Drugs

“…During the last two years, several hundred papers have been published that deal with various aspects of chiral separations. A few examples of them include ones that use high performance liquid chromatography, − gas chromatography, thin-layer chromatography, , capillary electrochromatography, − capillary electrophoresis − and micellar and related electrokinetic methods − to resolve many different enantiomeric compounds by use of a chiral stationary phase, a chiral additive, or a chiral derivatizing reagent. Summarized in Table are examples of some of the compounds where chiral purity was evaluated in either the bulk pharmaceutical or the formulated product using one of these approaches.…”

“…Among these are two reviews that discuss monolithic chiral stationary phases and the use of microscale chiral HPLC for process analysis . Likewise, four other papers have appeared that describe (1) the preparation of a permethyl-β-cyclodextrin bonded phase and (2) the online application of circular dichroic and NMR measurements to eliminate the need for authentic standards, the properties of eicosa- O -methyl-β-cyclodextrin isomers as capillary gas chromatographic chiral stationary phases, and the use of thin-layer chromatography for separating optically active pharmaceuticals . The first of the reviews presents information about both silica-based and organic monolithic materials and synthetic methods for preparing many different chiral forms of them.…”