Planar chromatography

Braithwaite, A.; Smith, Frederick M.

doi:10.1007/978-94-011-0599-6_3

Cited by 2 publications

(1 citation statement)

References 55 publications

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“…Planar chromatography is an analytical method, which depends on two separation mechanisms (14,15). One is based on the process of adsorption of the product to the stationary phase, such as Al 2 O 3 or silica-gel, and desorption of the molecules by the mobile phase or solvent.…”

Section: Discussionmentioning

confidence: 99%

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+

Faria

Buchpiguel

Marques

2015

Braz J Med Biol Res

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Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI)6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol:chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI)6]+ since impurities such as99mTc-reduced-hydrolyzed (RH),99mTcO4 - and [99mTc(cysteine)2]-complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.

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Section: Discussionmentioning

confidence: 99%

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+

Faria

Buchpiguel

Marques

2015

Braz J Med Biol Res

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Overview of High-Performance Thin Layer Chromatography and its Applications in Pesticide Analysis

Kandasamy

Muruganantham

Krishnaswami

et al. 2023

CAC

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In recent decades, increased demand for food has been caused by a rapid rise in the human population, which triggers agricultural intensification. To resist undesired pests from infecting crops, farmers widely utilize pesticides to improve agricultural production during the pre-harvest period. Despite the fact that pesticides cause a number of health risks, there is insufficient monitoring of these toxins. Therefore, it is important to develop a specific, accurate, and efficient method for determining the pesticides in varied samples in order to safeguard health against potential risks. Due to the lower concentrations of active compounds and their diversity of availability, it is challenging to detect pesticide residues in different samples. In this case, to effectively separate, identify, and accurately quantify pesticides at low concentrations in a variety of samples, a reliable analytical methodology is needed. Recently, the application of high-performance thin layer chromatography (HPTLC) offers a wider scope with excellent separation, identification, and quantitative/qualitative determination in pesticide analysis. In spite of their extremely low quantities, pesticide residues can be accurately and precisely identified using HPTLC. HPTLC has a number of benefits, such as easy sample preparation, automation, densitometry, and hyphenation, and is particularly well suited for identification and detection. Concerning this, the proposed review paper provides an overview of stationary phases, mobile phases, sample applicators, visualization, derivatization, and detection of HPTLC utilized for the identification and detection of pesticide residues in agriculture and environmental samples.

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Planar chromatography

Cited by 2 publications

References 55 publications

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+

Overview of High-Performance Thin Layer Chromatography and its Applications in Pesticide Analysis

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