Planar optical sensors: A tool for screening enzyme activity in high density cell arrays

Gerlach, Jochen; Pohn, Brigitte; Karl, Wolfgang; Scheideler, Marcel; Uray, Martina; Bischof, Horst; Schwab, Helmut; Klimant, Ingo

doi:10.1016/j.snb.2005.08.008

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…Facing the needs of directed evolution programs and of exploring metagenomes, a new high throughput enzyme screening system was developed [63] [64]. It was based on the optical detection by CCD camera of the microcolony array system with a fluorescent chemo-sensor and automated CDIA providing simultaneous time-resolved monitoring of enzyme activity of up to 7000 single microcolonies.…”

Section: Microcolonies and Biofilmsmentioning

confidence: 99%

Image Analysis in Microbiology: A Review

Pushchino¹

2016

JCC

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This review is focused on using computer image analysis as a means of objective and quantitative characterizing optical images of the macroscopic (e.g. microbial colonies) and the microscopic (e.g. single cell) objects in the microbiological research. This is the way of making many visual inspection assays more objective and less time and labor consuming. Also, it can provide new visually inaccessible information on relation between some optical parameters and various biological features of the microbial cultures. Of special interest is application of image analysis in fluorescence microscopy as it opens new ways of using fluorescence based methodology for single microbial cell studies. Examples of using image analysis in the studies of both the macroscopic and the microscopic microbiological objects obtained by various imaging techniques are presented and discussed.

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Section: Microcolonies and Biofilmsmentioning

confidence: 99%