Planned immunization of human volunteers with HLA‐DR incompatible lymphocytes

Madsen, M.; Johnsen, Hans Erik; Kristensen, Tom; Jørgensen, F.; Lamm, L. U.; Kissmeyer-Nielsen, F

doi:10.1111/j.1399-0039.1982.tb00327.x

Cited by 6 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunizing donors were selected among about 5,000 HLA-A and -B typed local blood donors in order to match their recipient as closely as possible for HLA-A, B, and C antigens and to be incompatible for one HLA-DR antigen. The detailed procedure of immunization has been published previously (Madsen et al 1982). In short, peripheral blood lymphocytes (PBL) were isolated from the selected donors under sterile conditions as described previously (Jrargensen & Lamm 1974).…”

Section: Immunizationmentioning

confidence: 99%

One‐way positive cellular reactions between two HLA‐A, B, C, D/DR genotypically identical brothers following active allogeneic immunization

Kristensen

Madsen

1983

Tissue Antigens

Self Cite

View full text Add to dashboard Cite

The HLA-D/DR region in man encodes major determinants which stimulate T lymphocytes to proliferation. The genetic organization of this region is apparently complex and is at present largely unknown. One obstacle is the scarcity and quality of available typing reagents. In an attempt to obtain high quality anti-DR sera, a series of active immunizations was performed between highly selected, healthy unrelated donors and recipients. One recipient (AR8) was immunized using cells incompatible for HLA-A2, B40 (w60), Cw3 and DIDRw6 and readily developed anti-A2 and B40 antibodies but no anti-C, DR, or other antibodies. When tested against his HLA genotypically fully identical brother using the cellular MLC, PLT, or CML techniques before immunization, results were mutually negative as expected. Following immunization, however, AR8 was able to mount MLC, PLT, and possibly CML responses against lymphocytes from the brother while the reverse combinations remained negative. When tested in the family the trait(s) thus identified seems to be maternally inherited. These results suggest the existence of minor histocompatibility determinants encoded from regions not closely linked to HLA. The brother of AR8 and the immunizing donor thus seem to share one or more determinants not possessed by AR8.

show abstract

Section: Immunizationmentioning

confidence: 99%

One‐way positive cellular reactions between two HLA‐A, B, C, D/DR genotypically identical brothers following active allogeneic immunization

Kristensen

Madsen

1983

Tissue Antigens

Self Cite

View full text Add to dashboard Cite

show abstract

“…Aarhus. Denmark with the objective of obtaining specific anti-11LA-DR antisera (Madsen et al 1982). Identified incompatibilities hetween v-olunteer and donor were: HLA-A2; BW (w60); Cw3; and DwiDRw6.…”

mentioning

confidence: 99%

Human cytotoxic, specific anti HLA‐A, ‐B, ‐C T‐lymphocyte clones are OKT3 positive, but may change their OKT4 and 8 phenotypes during culture

Kristensen

Malissen

1983

Tissue Antigens

Self Cite

View full text Add to dashboard Cite

Serological responses of melanoma patients to vaccines derived from allogeneic cultured melanoma cells

Livingston

Takeyama

Pollack

et al. 1983

Intl Journal of Cancer

View full text Add to dashboard Cite

Twenty melanoma patients were immunized with a whole-cell vaccine prepared from irradiated cells of the melanoma cell line SK-MEL-13. This line, derived from the melanoma of patient AH, expresses a differentiation antigen (initially defined by autologous antibody) which is restricted to melanomas and other cells of neural crest origin. The patients' sera were tested in rosetting assays before and after vaccination for antibodies against cell surface antigens of autologous cultured melanoma cells and SK-MEL-13, and the specificity of observed reactions was defined by absorption tests. Nine patients developed antibodies against autologous cultured melanoma cells. In only one case, patient DM, were these antibodies directed against the AH antigen. In the remaining patients, the antibodies were related to fetal bovine serum in the vaccine growth medium. All patients developed antibodies against cell surface HLA alloantigens of the immunizing cell, SK-MEL-13, as demonstrated by absorption analysis with B cells from the donor of SK-MEL-13 and by serological typing with a panel of HLA typed peripheral blood T cells and B-cell CLL cells. No specificity other than the AH antibody found in patient DM was detected after removal of alloantigens with B cells. We conclude that vaccines prepared from irradiated allogeneic melanoma cells expressing AH antigen, a demonstrably immunogenic differentiation antigen of the melanocyte lineage, are not effective in inducing AH antibody.

show abstract

Planned immunization of human volunteers with HLA‐DR incompatible lymphocytes

Cited by 6 publications

References 19 publications

One‐way positive cellular reactions between two HLA‐A, B, C, D/DR genotypically identical brothers following active allogeneic immunization

One‐way positive cellular reactions between two HLA‐A, B, C, D/DR genotypically identical brothers following active allogeneic immunization

Human cytotoxic, specific anti HLA‐A, ‐B, ‐C T‐lymphocyte clones are OKT3 positive, but may change their OKT4 and 8 phenotypes during culture

Serological responses of melanoma patients to vaccines derived from allogeneic cultured melanoma cells

Contact Info

Product

Resources

About