Plant-Based Antimicrobial Studies – Methods and Approaches to Study the Interaction between Natural Products

Vuuren, Sandy van; Viljoen, Alvaro

doi:10.1055/s-0030-1250736

Cited by 299 publications

(321 citation statements)

References 153 publications

(165 reference statements)

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“…Despite major toxicity concerns and adverse reactions among patients, QUIN remains one of the most commonly used antimalarial for therapy (van Vuuren and Viljoen, 2011). Moreover, this alkaloid was reported for the inhibition of the invasive ability of some bacteria, namely, staphylococci, which would protect the immunocompromised patients of being infected during the antimalarial treatment (Wolf et al, 2002;.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

Abreu

Serra

Borges

et al. 2014

Diagnostic Microbiology and Infectious Disease

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The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 ± 2.2 mg/L) and theophylline (IC50 = 100 ± 4.7 mg/L).

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Section: Discussionmentioning

confidence: 99%

Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

Abreu

Serra

Borges

et al. 2014

Diagnostic Microbiology and Infectious Disease

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“…It is argued to be advantageous over MIC assays as it can show effects over time (van Vuuren and Viljoen, 2011). These assays would be used if a promising compound/extract is identified following MIC assays.…”

Section: Death Kinetic (Time-kill) Assaysmentioning

confidence: 99%

“…Three of the studies in this review involved the clinically difficult to treat Gram-negative bacteria, two researched with Gram positive Staphylococcus aureus and two involved both Gramnegative and Gram-positive strains. Diffusion assay and measurement of zones of inhibition offer an indication of interactions but require cautious evaluation due to a multitude of influencing factors (van Vuuren and Viljoen, 2011), for example issues with solubility of compounds. As synergistic effects may occur at different concentration ratios, Heinrich et al (2012) report that the isobole method is the agreed method of choice.…”

Section: Synergy Directed Fractionationmentioning

confidence: 99%

Antimicrobial resistance and synergy in herbal medicine

Mundy

Pendry

Rahman

2016

Journal of Herbal Medicine

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Antimicrobial resistance (AMR) is a serious and growing threat to human health. The development of new antibiotics is limited and slow. The tradition of synergy in herbal medicine is being used as a source of research ideas. A literature review of antimicrobial research and plant synergy published in a five year period was carried out using online databases. The in vitro findings were that most of the research reported synergy both within plants and between plants and antibiotics.Whole plant extracts and combinations of compounds were shown to be more effective antimicrobials than isolated constituents. The discussion highlights that the in vitro herbal research findings are difficult to apply to practice and aren't progressing to clinical trials.Collaborative, innovative, inter-disciplinary clinical research is recommended.

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“…Multidrug therapy has become especially important in the fight against infectious diseases. Several approaches used to evaluate the activities of antimicrobial drug and natural product combinations have been reviewed extensively [7]. Additionally, there have been reports on the reduction in the occurrence of resistance of antimicrobial strains to crude rather than single active compounds, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, there have been reports on the reduction in the occurrence of resistance of antimicrobial strains to crude rather than single active compounds, e.g. from antimalarial drug research [7,8].…”

Section: Introductionmentioning

confidence: 99%

An update on the strategies in multicomponent activity monitoring within the phytopharmaceutical field

Gostner¹,

Wrulich²,

Jenny³

et al. 2012

Z Phytother

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Plant-Based Antimicrobial Studies – Methods and Approaches to Study the Interaction between Natural Products

Cited by 299 publications

References 153 publications

Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus

Antimicrobial resistance and synergy in herbal medicine

An update on the strategies in multicomponent activity monitoring within the phytopharmaceutical field

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