Plant Biotechnologies Applied to a Forest Tree, the American Red Oak (Quercus Rubra L.)

Rancillac, M.; Klinguer, Agnès; Klinguer, Serge

doi:10.17660/actahortic.1991.289.86

Cited by 8 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shoot-tip necrosis is a common physiological disorder with some in vitro cultures of tree species (Vieitez et al 1989). Explants cultured in a vertical orientation exhibited shoot growth and apical necrosis in Q. rubra (Vieitez et al 1993), and similar observations were reported by Rancillac et al (1991). In the present study, a low concentration of BA (0.44 μM) and GA 3 (0.29 μM) promoted shoot elongation and prevented shoot-tip dormancy and necrosis.…”

Section: Resultssupporting

confidence: 89%

“…In vitro propagation of Q. rubra has been reported using stem segments and embryonic axes, but the in vitro raised microshoots did not produce roots (Rancillac et al 1991;Schwarz and Schlarbaum 1993). Vieitez et al (1993) achieved plant regeneration in Q. rubra using shoot tip and nodal segments from both juvenile and adult trees, but shoot cultures exhibited arrest of growth and apical necrosis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro propagation of northern red oak (Quercus rubra L.)

Vengadesan

Pijut

2009

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

In vitro propagation of northern red oak (Quercus rubra) shoots was successful from cotyledonary node explants excised from 8-wk-old in vitro grown seedlings. Initially, four shoots per explant were obtained on Murashige and Skoog (MS) medium supplemented with 4.4 μM 6-benzylaminopurine (BA), 0.45 μM thidiazuron (TDZ), and 500 mg l −1 casein hydrolysate (CH) with a regeneration frequency of 64.7% after 3 wk. Subculturing explants (after harvesting shoots) to fresh treatment medium significantly increased shoot bud regeneration (16.6 buds per explant), but the buds failed to develop into shoots. A higher percentage (73.3%) of the explants regenerated four shoots per explant on woody plant medium (WPM) supplemented with 4.4 μM BA, 0.29 μM gibberellic acid (GA 3 ), and 500 mg l −1 CH after 3 wk. Explants subcultured to fresh treatment medium after harvesting shoots significantly increased shoot regeneration (16 shoots per explant). Shoot elongation was achieved (4 cm) when shoots were excised and cultured on WPM supplemented with 0.44 μM BA and 0.29 μM GA 3 . In vitro regenerated shoots were rooted on WPM supplemented with 4.9 μM indole-3-butyric acid. A higher percentage regeneration response and shoot numbers per explant were recorded on WPM supplemented with BA and GA 3 , than on MS medium containing BA and TDZ. Lower concentrations of BA and GA 3 were required for shoot elongation and prevention of shoot tip necrosis. Each cotyledonary node yielded approximately 20 shoots within 12 wk. Rooted plantlets were successfully acclimatized.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

In vitro propagation of northern red oak (Quercus rubra L.)

Vengadesan

Pijut

2009

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…Quercus rubra stem, leaf petiole, and leaf disc explants were used to induce somatic embryos, and embryos with cotyledons and a root pole were obtained only from leaf discs cultured on MS medium supplemented with 5.4 lM NAA and 0.09 lM BA after 6-8 weeks under a 16 h photoperiod without subculture, but the embryos failed to undergo apical bud elongation (Rancillac et al 1991). Rancillac et al (1996) also achieved somatic embryogenesis from leaf discs collected from juvenile NRO plants on MS medium supplemented with 5.4 lM NAA and 0.09 lM BA enriched with casein hydrolysate, and reported that light was necessary for somatic embryogenesis.…”

Section: Resultsmentioning

confidence: 99%

“…Direct and indirect embryogenesis were induced from immature zygotic embryo explants using 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA), but plantlet regeneration was low (Gingas and Lineberger 1989). Leaf discs from 1.5 to 3-month-old seedlings were used as explants to induce embryos using a-naphthaleneacetic acid (NAA) and BA, but the majority of embryos did not have a viable apical bud and plantlet survival was low (Rancillac et al 1991(Rancillac et al , 1996. Callus cultures obtained from male catkins using BA or 2,4-D did not regenerate embryos in NRO (Gingas 1991).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Vengadesan

Pijut

2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A somatic embryogenesis protocol for plant regeneration of northern red oak (Quercus rubra) was established from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium (MS) containing 3% sucrose, 0.24% Phytagel TM , and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) after 4 weeks of culture in darkness. A higher response (66%) of embryogenic callus was induced on 0.45 lM 2,4-D. Higher numbers of globular-(31), heart-(17), torpedo-(12), and cotyledon-stage (8) embryos per explant were obtained by culturing embryogenic callus on MS with 3% sucrose, 0.24% Phytagel TM , and devoid of growth regulators after 8 weeks culture in darkness. Continuous sub-culturing of embryogenic callus on medium containing 2,4-D yielded only compact callus. Desiccation of embryos for 3 days in darkness at 25 ± 2°C followed by cold storage at 4°C in darkness for 8 weeks favored embryo germination and development of plantlets. Cotyledon-stage embryos subjected to desiccation and chilling treatment cultured on MS with 3% sucrose, 0.24 Phytagel TM , 0.44 lM 6-benzylaminopurine (BA), and 0.29 lM gibberellic acid germinated at a higher frequency (61%) than with 0.44 lM BA alone and control cultures. Germinated plantlets developed a shoot and root, were acclimatized successfully, and maintained in a growth room for plantlet development.

show abstract

“…These authors concluded that episodic growth in culture was a significant factor in the cultures' demise. In the case of Q. rubra, difficulties were encountered even in micropropagation with juvenile seedling material (Rancillac et al 1991;Vengadesan and Pijut 2007) where shoot tip necrosis, dormancy and decline of shoot growth were common problems described (McCown 2000). Although the micropropagation from epicormic shoots of red oaks was also reported (Vieitez et al 1993a;Sánchez et al 1996), sustainable reliable results have been inconsistent for several genotypes indicating that genotypic effects need to be considered in terms of physiological requirements for maximum shoot proliferation.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Viéitez

Corredoira

Ballester

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

North American oak species, with their characteristic strong episodic seasonal shoot growth, are highly problematic for clonal micropropagation, resulting in the inability to achieve a stabilized shoot multiplication stage. The potential for initiating and proliferating shoot cultures derived from Quercus alba, Q. bicolor and Q. rubra explants was investigated, and a micropropagation method for these species was developed. Branch segments from 6 to 7-year-old trees were forced-flushed and the forced shoots were used as source of explants for culture initiation. A consistent shoot multiplication stage was achieved, in 13 of the 15 genotypes established in vitro, although marked differences occurred in explants from different genotypes/species. The control of efficient shoot multiplication involved the culture of decapitated shoots in a stressful horizontal position on cytokinin-containing medium with a sequence of transfers within a 6-week subculture cycle, which was beneficial to overcoming the episodic character of shoot growth. During each subculture cycle, the horizontally placed explants were cultured on media containing 0.2 mg l -1 benzyladenine (BA) for 2 weeks with two successive transfers (2 weeks each) to fresh medium with 0.1 mg l -1 BA, giving a 6-week subculture cycle. The general appearance and vigor of Q. alba and Q. bicolor shoot cultures were improved by the inclusion of both 0.1 mg l -1 BA and 0.5 mg l -1 zeatin in the medium used for the second transfer within the 6-week subculture cycle. Addition of AgNO 3 (3 mg l -1 ) to the shoot proliferation medium of Q. rubra had a significant positive effect on shoot development pattern by reducing deleterious symptoms, including shoot tip necrosis and early senescence of leaves. The three species showed acceptable in vitro rooting rates by culturing microcuttings in medium containing 25 mg l -1 indolebutyric acid for 48 h with subsequent transfer to auxin-free medium supplemented with 0.4% activated charcoal. Although an initial 5-day dark period generally improved the rooting response, it was detrimental to the quality of regenerated plantlets. However, activated charcoal stimulated not only the rooting frequencies, but it also enhanced plant quality, as evidenced by root, shoot and leaf growth.

show abstract

Plant Biotechnologies Applied to a Forest Tree, the American Red Oak (Quercus Rubra L.)

Cited by 8 publications

References 0 publications

In vitro propagation of northern red oak (Quercus rubra L.)

In vitro propagation of northern red oak (Quercus rubra L.)

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Contact Info

Product

Resources

About