Plant biotechnology in South Africa: Micropropagation research endeavours, prospects and challenges

Moyo, Mack; Bairu, Michael W.; Amoo, Stephen O.; Staden, J. Van

doi:10.1016/j.sajb.2011.06.003

Cited by 45 publications

(18 citation statements)

References 171 publications

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“…Segundo , a técnica permite a multiplicação rápida de genótipos, selecionados por maior produtividade, uniformidade e desempenho no campo, sendo uma importante ferramenta na propagação de mudas sadias, em tempo e espaço físico reduzidos. Diversas espécies de plantas medicinais têm sido micropropagadas com o intuito de aumentar a produção de metabólitos de interesse, quer seja por estímulo químico ou por manipulação das condições no microambiente da cultura in vitro (Moyo et al, 2011), a exemplo de algumas lenhosas, como Ephedra procera C.A. Mey., Syzygium cordatum Hochst.…”

Section: Introductionunclassified

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

et al. 2013

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In vitro multiplication of Tapirira guianensis Aubl. (Anacardiaceae)Tapirira guianensis is of great medicinal, ecological and socio-economical importance occurring throughout the Brazilian territory. The objective of this study was to establish and determine the best conditions for the in vitro multiplication of T. guianensis. The explants nodal, cotyledonary and epicotyl segments from seedlings germinated in vitro were tested in diverse concentrations of 6-benzilaminopurine (BAP) and/or napthaleneacetic acid (NAA) in medium WPM. The following parameters were evaluated: the percentage of responsive explants, the number of shoots and buds, and the length of shoots and shoot dry mass at 30 and 60 days after inoculation. The cotyledonary segment, under these conditions, is the most suitable explant for the multiplication, with no induction of adventitious buds in epicotyl. The treatment with 1.0 mg L -1 BAP in the absence of NAA is the most responsive to the T. guianensis regeneration.Key words: medicinal woody, in vitro propagation, regeneration, direct organogenesis. Tapirira guianensis possui grande relevância medicinal, ecológica e socioeconômica, ocorrendo em todo o territó-rio brasileiro. O objetivo deste estudo foi estabelecer e determinar as melhores condições para a sua multiplicação in vitro. Os explantes, segmentos nodais, cotiledonares e epicótilos, oriundos de plântulas germinadas in vitro, foram testados em concentrações de 6-benzilaminopurina (BAP) e, ou, ácido naftalenoacético (ANA), em meio de cultura WPM. As características avaliadas foram a percentagem de explantes responsivos, o número de brotos e de gemas, o comprimento dos brotos e a matéria seca da parte aérea, aos 30 e 60 dias após inoculação. Foi observado que o segmento cotiledonar, nas condições deste estudo, foi o explante mais indicado para a multiplicação, não havendo indução de brotos adventícios nos epicótilos. O tratamento com 1,0 mg L -1 de BAP na ausência de ANA é o mais responsivo para a regeneração de T. guianensis.Palavras-chave: lenhosa medicinal, propagação in vitro, regeneração, organogênese direta.

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Section: Introductionunclassified

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

et al. 2013

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“…The successful production of transgenic plants requires an adequate infrastructure, expertise in tissue culture and molecular biology, and sustainable funding to cover the high cost of such research (Brink et al, 1998). Research in plant biotechnology is playing a crucial role in the production and conservation of plant-based resources globally (Moyo et al, 2011). Germplasm conservation and cryopreservation ensure genetic stability and retention of biosynthetic potential (Rajasekharan and Prakashkumar, 2010).…”

Section: Integration Approachmentioning

confidence: 99%

Role of biotechnology in the conservation of rare, threatened and endangered medicinal plant species in the Kingdom of Eswatini (Swaziland)

Kunene¹,

Masarirambi²

2018

Adv Med Plant Res

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The use of indigenous medicinal plants by people is widespread in the Kingdom of Eswatini and the Southern Africa region as a whole. However, due to over exploitation, and for some other reasons like climate change, some indigenous medicinal plants have become endangered and are threatened with extinction. Subsequent loss of biodiversity is at stake. Urgent intervention is therefore, required to conserve them. The purpose of this study was to document plant biotechnology techniques which can be used in conservation of rare, endangered and potentially threatened medicinal plants of crucial importance in the country and region. Various ways of conservation through plant biotechnology are discussed.

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“…Plant tissue culture provides valuable tool for synthesizing same range of chemicals as that of natural plant as well as novel compounds are also synthesized via biotransformations. [68] The ability of A. rhizogenes to induce hairy roots in a range of medicinal plants has been exploited as a source of root-derived pharmaceutical compounds. [72] Over the past two decades, the concept of plant-based production of high volume-based pharmaceutical proteins such as vaccines and antibodies has received growing research interest and offered critical advantages over traditional bacterial and mammalian cell-based systems.…”

Section: Future Prospectsmentioning

confidence: 99%

In vitro production of alkaloids: Factors, approaches, challenges and prospects

Ahmad¹,

Garg²,

Tamboli³

et al. 2013

Phcog Rev

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The wide diversity of plant secondary metabolites is largely used for the production of various pharmaceutical compounds. In vitro cell tissue or organ culture has been employed as a possible alternative to produce such industrial compounds. Tissue culture techniques provide continuous, reliable, and renewable source of valuable plant pharmaceuticals and might be used for the large-scale culture of the plant cells from which these secondary metabolites can be extracted. Alkaloids are one of the most important secondary metabolites known to play a vital role in various pharmaceutical applications leading to an increased commercial importance in recent years. The tissue culture techniques may be utilized to improve their production of alkaloids via somaclonal variations and genetic transformations. The focus of this review is toward the application of different tissue culture methods/techniques employed for the in vitro production of alkaloids with a systematic approach to improve their production.

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Plant biotechnology in South Africa: Micropropagation research endeavours, prospects and challenges

Cited by 45 publications

References 171 publications

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

Role of biotechnology in the conservation of rare, threatened and endangered medicinal plant species in the Kingdom of Eswatini (Swaziland)

In vitro production of alkaloids: Factors, approaches, challenges and prospects

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