Plant cell culture technology in the cosmetics and food industries: current state and future trends

Eibl, Regine; Meier, Philipp; Stutz, Irène; Schildberger, David; Hühn, Tilo; Eibl, Dieter

doi:10.1007/s00253-018-9279-8

Cited by 171 publications

(130 citation statements)

References 90 publications

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“…Regarding plant cell culture, several recent reviews bring a new light to in vitro culture, for investigation purposes as well as for its uses as a valuable platform for high-value metabolite production (Wilson and Roberts, 2012;Moscatiello et al, 2013;Ochoa-Villarreal et al, 2015;Eibl et al, 2018). Examples are scattered in the literature with in vitro cultures producing compounds of interest either using dedifferentiated cells from callus or undifferentiated cells from meristematic cambial cells.…”

Section: Lautié Et Al Maximizing Explored Plant Chemical Diversitymentioning

confidence: 99%

“…As seen above, many examples can be found in the literature, but the remaining question is how much the methodology varied from one example to the next in order to obtain such callus and then to obtain such cell suspensions producing the desired compound. Some general procedures for the establishment of dedifferentiated plant cell suspension cultures exist (Mustafa et al, 2011;Eibl et al, 2018) but with many specific adaptations in the function of species, organs of origins, and culture conditions. Probably because the authors concentrated mainly on the productivity and yield of the targeted compound and not on a larger picture applicable to a more general view of accessing and testing the chemical diversity of plants.…”

Section: Lautié Et Al Maximizing Explored Plant Chemical Diversitymentioning

confidence: 99%

“…The use of plant cell and organ culture for the production at the industrial scale of compounds with superior added value has been reported in various reviews (Wilson and Roberts, 2012;Imseng et al, 2014;Eibl et al, 2018). These reviews cited a large range of applications from the pharmaceutical area (suspension cells of Pacific yew in 75m 3 stirred bioreactors delivering 500kg/ year of paclitaxel) to the cosmetic and food industries like cell cultures of Malus domestica grown in 50 to 100 L production bioreactors.…”

Section: Industrial Scale Production: Synthetic Biology and Organic Smentioning

confidence: 99%

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Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Lautie

Russo

Ducrot³

2020

Front. Pharmacol.

166

120

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The screening and testing of extracts against a variety of pharmacological targets in order to benefit from the immense natural chemical diversity is a concern in many laboratories worldwide. And several successes have been recorded in finding new actives in natural products, some of which have become new drugs or new sources of inspiration for drugs. But in view of the vast amount of research on the subject, it is surprising that not more drug candidates were found. In our view, it is fundamental to reflect upon the approaches of such drug discovery programs and the technical processes that are used, along with their inherent difficulties and biases. Based on an extensive survey of recent publications, we discuss the origin and the variety of natural chemical diversity as well as the strategies to having the potential to embrace this diversity. It seemed to us that some of the difficulties of the area could be related with the technical approaches that are used, so the present review begins with synthetizing some of the more used discovery strategies, exemplifying some key points, in order to address some of their limitations. It appears that one of the challenges of natural product-based drug discovery programs should be an easier access to renewable sources of plant-derived products. Maximizing the use of the data together with the exploration of chemical diversity while working on reasonable supply of natural product-based entities could be a way to answer this challenge. We suggested alternative ways to access and explore part of this chemical diversity with in vitro cultures. We also reinforced how important it was organizing and making available this worldwide knowledge in an "inventory" of natural products and their sources. And finally, we focused on strategies based on synthetic biology and syntheses that allow reaching industrial scale supply. Approaches based on the opportunities lying in untapped natural plant chemical diversity are also considered.

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Section: Lautié Et Al Maximizing Explored Plant Chemical Diversitymentioning

confidence: 99%

Section: Lautié Et Al Maximizing Explored Plant Chemical Diversitymentioning

confidence: 99%

Section: Industrial Scale Production: Synthetic Biology and Organic Smentioning

confidence: 99%

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Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Lautie

Russo

Ducrot³

2020

Front. Pharmacol.

166

120

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“…Cell factories, using production hosts ranging from bacterial, fungal, mammalian, and plant cells, are used to make important industrial products including pharmaceuticals, proteins, commodity chemicals, and biofuels [1][2][3][4] . As cellular engineering becomes increasingly sophisticated, incorporating longer and more complex heterologous metabolic pathways, the likelihood of crosstalk with native cellular functions in the host cell also increases.…”

Section: Introductionmentioning

confidence: 99%

Repurposing the yeast peroxisome to compartmentalize a toxic enzyme enables improved (S)-reticuline production

Grewal¹,

Samson²,

Baker

et al. 2020

Preprint

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Eukaryotic cells compartmentalize metabolic pathways in organelles to achieve optimal reaction conditions and avoid crosstalk with other factors in the cytosol. Increasingly, engineers are researching ways in which synthetic compartmentalization could be used to address challenges in metabolic engineering. Here, we identified that norcoclaurine synthase (NCS), the enzyme which catalyzes the first committed reaction in benzylisoquinoline alkaloid (BIA) biosynthesis, is toxic when expressed cytosolically in Saccharomyces cerevisiae and, consequently, restricts (S)-reticuline production. We developed a compartmentalization strategy that alleviates NCS toxicity while promoting increased (S)-reticuline titer, achieved through efficient targeting of toxic NCS to the peroxisome while, crucially, taking advantage of the free flow of metabolite substrates and product across the peroxisome membrane. We identified that peroxisome protein capacity in S. cerevisiae becomes a limiting factor for further improvement of BIA production and demonstrate that expression of engineered transcription factors can mimic the oleate response for larger peroxisomes, further increasing BIA titer without the requirement for peroxisome induction with fatty acids. This work specifically addresses the challenges associated with toxic NCS expression and, more broadly, highlights the potential for engineering organelles with desired characteristics for metabolic engineering.

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“…The utilization of bioreactor is an exciting preposition in medicinal plant biotechnology for shoot multiplication, and production of bioactive metabolites has recently progressed exhibiting a considerable development towards cultivation of plants for metabolite production using cost effective, easily available bioreactor devices [6][7][8][9][10]. The potential of in vitro regenerated shoot cultures for the production of bacosides [1,[11][12][13] along with suitability of liquid medium to grow shoots has been analyzed [14,15], which provides the basis for large scale cultivation in bioreactor.…”

Section: Introductionmentioning

confidence: 99%

Suitability of bench scale bioreactor system for shoot biomass production and bacoside biosynthesis from Bacopa monnieri (L.)

Sharma

Koul

Ahuja

et al. 2019

Engineering in Life Sciences

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According to folklore, Bacopa monnieri commonly called as Brahmi is known for its cognitive enhancing properties. The plant is found abundantly in wetlands but the drug content (bacosides) is very low (0.2%), therefore, alternative biotechnological protocols are highly needed to supplement the constant source of this valuable plant material which produces stable amounts of bacosides. The present study was conducted to explore the application of different culture systems for cultivation of shoot biomass and maximization of biologically active bacoside biosynthesis in this medicinally important plant. Shoot cultures of Bacopa were cultivated in two different modified benchtop bioreactors: glass bottle bioreactor and balloon type bubble bioreactor and compared with those grown in traditional Erlenmeyer agitated flask. The shoots cultivated in the balloon type bubble bioreactor system showed excellent growth (growth index 796.47 ± 17.27 fresh weight and 395.55 ± 7.55 dry weight) as compared to glass bottle bioreactor system (growth index 488.17 ± 14.4 fresh weight and 327.79 ± 6.64 dry weight) and agitated flask (growth index 363.43 ± 11 fresh weight and 304.22 ± 6.76 dry weight). Furthermore, bacosides produced by shoot cultures cultivated in the balloon type bubble bioreactor (321.95 ± 17.14 mg/L) and glass bottle bioreactor (180.18 ± 6.25 mg/L) configurations were ∼2.78 fold and ∼1.55 fold higher than that recorded in agitated flask cultures (115.7 ± 3.84 mg/L). The balloon type bubble bioreactor system was found to be advantageous for enhancing B. monnieri shoot biomass and bacoside biosynthesis along with ensuring a successful protocol for continuous supply.

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Plant cell culture technology in the cosmetics and food industries: current state and future trends

Cited by 171 publications

References 90 publications

Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes

Repurposing the yeast peroxisome to compartmentalize a toxic enzyme enables improved (S)-reticuline production

Suitability of bench scale bioreactor system for shoot biomass production and bacoside biosynthesis from Bacopa monnieri (L.)

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