Plant Cell Organelle Proteomics in Response to Abiotic Stress

Abstract

Proteomics is one of the finest molecular techniques extensively being used for the study of protein profiling of a given plant species experiencing stressed conditions. Plants respond to a stress by alteration in the pattern of protein expression, either by up-regulating of the existing protein pool or by the synthesizing novel proteins primarily associated with plants antioxidative defense mechanism. Improved protein extraction protocols and advance techniques for identification of novel proteins have been standardized in different plant species at both cellular and whole plant level for better understanding of abiotic stress sensing and intracellular stress signal transduction mechanisms. In contrast, an in-depth proteome study of subcellular organelles could generate much detail information about the intrinsic mechanism of stress response as it correlates the possible relationship between the protein abundance and plant stress tolerance. Although a wealth of reviews devoted to plant proteomics are available, review articles dedicated to plant cell organelle proteins response under abiotic stress are very scanty. In the present review, an attempt has been made to summarize all significant contributions related to abiotic stresses and their impacts on organelle proteomes for better understanding of plants abiotic stress tolerance mechanism at protein level. This review will not only provide new insights into the plants stress response mechanisms, which are necessary for future development of genetically engineered stress tolerant crop plants for the benefit of humankind, but will also highlight the importance of studying changes in protein abundance within the cell organelles in response to abiotic stress.

Lipids and proteins—major targets of oxidative modifications in abiotic stressed plants

“…In response to abiotic stress factors, plants may also accumulate and/or synthesize protein types (such as heat shock proteins (HSPs), zinc-finger proteins, mitogen-activated protein kinase (MAPK), late embryogenesis abundant (LEA) proteins; dehydrins, late embryogenesis abundant (LEA) proteins, chaperones, etc.). These proteins have been evidenced to help plants to run cellular metabolism and eventually to tolerate potential abiotic stress impacts (reviewed by Timperio et al 2008;Fatehi et al 2012;Hossain et al 2012;Ghosh and Xu 2014). However, owing to their great abundance and high rate constants of a range of reactive radicals, proteins are one of the major cellular constituents targeted by ROS (Davies 2005).…”

MS based proteomic approaches for analysis of barley malt

“…2) (Bolwell et al, 2004;Cánovas et al, 2004;Figeys, 2003;Hennig, 2007) including studies of the relationships between proteins and plant development, seed maturation, responses to biotic and abiotic stresses and human allergies (e.g. Chen and Harmon, 2006;Hossain et al, 2012;Komatsu et al, 2012;Kosová et al, 2011;Sergeant and Renaut, 2010;Taká c et al, 2011;Timperio et al, 2008;Zhang et al, 2012). Many plants have been studied including A. thaliana, tobacco, vegetables, fruits and cereals.…”

Proteomics of Important Food Crops in the Asia Oceania Region: Current Status and Future Perspectives

“…We recently reviewed plant cell organelle proteomics in response to abiotic stress 180 . A number of subcellular proteomics studies (gel-free or gel based) on soybean have been already reported.…”