Plant Cell Redox Homeostasis and Reactive Oxygen Species

Trchоunian, Armen; Petrosyan, Margarit; Sahakyan, Naira

doi:10.1007/978-3-319-44081-1_2

Cited by 52 publications

(24 citation statements)

References 57 publications

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“…before 3 h) or may be masked by an efficient activation of scavenging or detoxifying mechanisms, such as CAT activity. To regulate the levels of H 2 O 2 , seeds have a ROS‐scavenging mechanism directed by both non‐enzymatic and enzymatic H 2 O 2 scavengers (Hossain et al , Trchounian et al ). In particular, the role of SA in the regulation of seed germination and dormancy may be associated with the regulation of the ROS levels in the seed.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal variations in salicylic acid and hydrogen peroxide in sunflower seeds during transition from dormancy to germination

Vigliocco

Bel

Pérez-Chaca

et al. 2019

Physiologia Plantarum

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Phytohormones and reactive oxygen species mediate processes such as germination and dormancy. The elucidation of the physiological and biochemical events implicated in the transition from dormancy to germination in different plant species such as sunflower becomes a topic of interest. In this study, we investigated the spatiotemporal variation of salicylic acid (SA), hydrogen peroxide (H 2 O 2 ) and the activity of two antioxidant enzymes (catalase, CAT -EC 1.11.1.6 and ascorbate peroxidase -EC 1.11.1.11) in embryonic axis and cotyledons of dry and imbibed seeds of dormant (B123) and non-dormant (B91) sunflower lines. The results showed that embryonic axis had higher level of SA and H 2 O 2 than cotyledons in both lines. In dry seeds, B123 embryo (embryonic axis + cotyledons) showed a higher SA content than B91. After dry storage at room temperature, SA decreased in B123 embryos to a value close to that registered in B91 embryos. B123 embryonic axis of dry seeds presented higher H 2 O 2 levels than B91. Dry storage led to an increase of H 2 O 2 levels and a decrease of CAT activity in B123 embryonic axis. During imbibition, B123 seeds stored for 33 days displayed an increase in SA level in the embryonic axis (3 h of imbibition) and this lower level correlated with a decrease in H 2 O 2 (6 h of imbibition). Thus, the embryo-imposed dormancy in B123 dry seeds was associated with high levels of SA and low H 2 O 2 , whereas the dormancy release was linked with SA decrease and increase of H 2 O 2 as a consequence of lower CAT activity.

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Section: Discussionmentioning

confidence: 99%

Spatiotemporal variations in salicylic acid and hydrogen peroxide in sunflower seeds during transition from dormancy to germination

Vigliocco

Bel

Pérez-Chaca

et al. 2019

Physiologia Plantarum

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“…The environment of molecular oxygen (O 2 ) is generally inactive due to its electron configuration [37]. But the unbalanced metabolism of O 2 can lead to the production of ROS, which include both free radicals (O 2 ∙− , superoxide radical; OH ∙ , hydroxyl radical; HO 2 ∙ , perhydroxyl radical; and RO ∙ , alkoxy radicals) and nonradical molecules (H 2 O 2 , hydrogen peroxide; and 1 O 2 , singlet oxygen) [5, 15, 38].…”

Section: The Metabolism Of Ros In Plantsmentioning

confidence: 99%

The Roles of Environmental Factors in Regulation of Oxidative Stress in Plant

Xie

Chen

et al. 2019

BioMed Research International

297

165

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Exposure to a variety of environmental factors such as salinity, drought, metal toxicity, extreme temperature, air pollutants, ultraviolet-B (UV-B) radiation, pesticides, and pathogen infection leads to subject oxidative stress in plants, which in turn affects multiple biological processes via reactive oxygen species (ROS) generation. ROS include hydroxyl radicals, singlet oxygen, and hydrogen peroxide in the plant cells and activates signaling pathways leading to some changes of physiological, biochemical, and molecular mechanisms in cellular metabolism. Excessive ROS, however, cause oxidative stress, a state of imbalance between the production of ROS and the neutralization of free radicals by antioxidants, resulting in damage of cellular components including lipids, nucleic acids, metabolites, and proteins, which finally leads to the death of cells in plants. Thus, maintaining a physiological level of ROS is crucial for aerobic organisms, which relies on the combined operation of enzymatic and nonenzymatic antioxidants. In order to improve plants’ tolerance towards the harsh environment, it is vital to reinforce the comprehension of oxidative stress and antioxidant systems. In this review, recent findings on the metabolism of ROS as well as the antioxidative defense machinery are briefly updated. The latest findings on differential regulation of antioxidants at multiple levels under adverse environment are also discussed here.

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“…Actually, in a normal and healthy body there is a balance between reactive oxygen species formation or free radical and endogenous antioxidant defense mechanisms [8]. In addition, the ROS are mostly removed by endogenous antioxidants enzymes, including catalase (CAT) and glutathione peroxidase (GPx) [9,10].…”

Section: Introductionmentioning

confidence: 99%

Thunbergia laurifolia Leaf Extract Increased Levels of Antioxidant Enzymes and Protected Human Cell-Lines In Vitro Against Cadmium

et al. 2020

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Thunbergia laurifolia or Rang Jued has been used as an herbal tea and in folk medicine as a detoxifying agent. Cd contamination is globally widespread and a serious public health problem. The aim of this study was to determine the endogenous antioxidant enzyme activities and malondialdehyde (MDA) production of the crude dried extract (CDE) of T. laurifolia leaves, using human embryonic kidney (HEK293) and human liver (HepG2) cells as in vitro models. Moreover, the cytotoxicity including anti-cadmium (Cd) toxicity in both cells were measured. The experimental design had 3 treatment groups with combined, pre-, and post-treatments for investigating the anti-Cd toxicity, and cell viability was determined with MTT test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). The CDE showed low cytotoxicity and increased catalase (CAT) and glutathione peroxidase (GPx) activities with decreased malondialdehyde (MDA) levels in both cell types. It was found that the CDE protected against Cd-induced toxicity in both cell types, and a synergistic combination therapy effect was seen when CaNa2EDTA, a chelating agent, was applied. Therefore, CDE can protect against Cd-induced oxidative stress in cells, possibly due to its antioxidant properties. Moreover, using the extract or drinking the herbal tea together with chelating agent should have an efficacy advantage over using the CDE or the chelating agent singly.

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Plant Cell Redox Homeostasis and Reactive Oxygen Species

Cited by 52 publications

References 57 publications

Spatiotemporal variations in salicylic acid and hydrogen peroxide in sunflower seeds during transition from dormancy to germination

Spatiotemporal variations in salicylic acid and hydrogen peroxide in sunflower seeds during transition from dormancy to germination

The Roles of Environmental Factors in Regulation of Oxidative Stress in Plant

Thunbergia laurifolia Leaf Extract Increased Levels of Antioxidant Enzymes and Protected Human Cell-Lines In Vitro Against Cadmium

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