Plant commensal type VII secretion system causes iron leakage from roots to promote colonization

Liu, Yunpeng; Shu, Xia Xia; Chen, Lin; Zhang, Huihui; Feng, Haichao; Sun, Xiaorong; Xiong, Qin; Li, Guangqi; Xun, Weibing; Xu, Zhihui; Zhang, Nan; Pieterse, Corné M. J.; Shen, Qirong; Zhang, Ruifu

doi:10.1038/s41564-023-01402-1

Cited by 30 publications

(8 citation statements)

References 63 publications

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“…These are organized in the same cluster and involve the crucial proteins for secreting conserved membrane component proteins EccB, EccC, EccD, and S8 family serine peptidase (MycP), which were similar to the T7SS gene cluster of other Gram-positive bacteria: Mycobacterium , Streptomyces , Micromonospora , Bifidobacterium , Bacillus , and Streptococcus ( Fyans et al., 2013 ; Houben et al., 2014 ; Trujillo et al., 2014 ; Rivera-Calzada et al., 2021 ). This secretion system plays an essential role in promoting the colonization of niches and host–microbe interactions between members in Actinobacteria and Firmicutes ( Abdallah et al., 2007 ; Liu et al., 2023 ). Two genes, tadA and tadB , in Amycolatopsis strain MEP2-6 T were identified as components of the Type II secretion system (T2SS), which encodes the TadA family conjugal transfer-associated ATPase and tight adherence protein B, respectively.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

Wannawong,

Mhuantong,

Macharoen

et al. 2024

Front. Plant Sci.

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A novel endophytic actinomycete, strain MEP2-6T, was isolated from scab tissues of potato tubers collected from Mae Fag Mai Sub-district, San Sai District, Chiang Mai Province, Thailand. Strain MEP2-6T is a gram-positive filamentous bacteria characterized by meso-diaminopimelic acid in cell wall peptidoglycan and arabinose, galactose, glucose, and ribose in whole-cell hydrolysates. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and hydroxy-phosphatidylethanolamine were the major phospholipids, of which MK-9(H6) was the predominant menaquinone, whereas iso-C16:0 and iso-C15:0 were the major cellular fatty acids. The genome of the strain was 10,277,369 bp in size with a G + C content of 71.7%. The 16S rRNA gene phylogenetic and core phylogenomic analyses revealed that strain MEP2-6T was closely related to Amycolatopsis lexingtonensis NRRL B-24131T (99.4%), A. pretoriensis DSM 44654T (99.3%), and A. eburnea GLM-1T (98.9%). Notably, strain MEP2-6T displayed 91.7%, 91.8%, and 87% ANIb and 49%, 48.8%, and 35.4% dDDH to A. lexingtonensis DSM 44653T (=NRRL B-24131T), A. eburnea GLM-1T, and A. pretoriensis DSM 44654T, respectively. Based on phenotypic, chemotaxonomic, and genomic data, strain MEP2-6T could be officially assigned to a novel species within the genus Amycolatopsis, for which the name Amycolatopsis solani sp. nov. has been proposed. The type of strain is MEP2-6T (=JCM 36309T = TBRC 17632T = NBRC 116395T). Amycolatopsis solani MEP2-6T was strongly proven to be a non-phytopathogen of potato scab disease because stunting of seedlings and necrotic lesions on potato tuber slices were not observed, and there were no core biosynthetic genes associated with the BGCs of phytotoxin-inducing scab lesions. Furthermore, comparative genomics can provide a better understanding of the genetic mechanisms that enable A. solani MEP2-6T to adapt to the plant endosphere. Importantly, the strain smBGCs accommodated 33 smBGCs encoded for several bioactive compounds, which could be beneficially applied in the fields of agriculture and medicine. Consequently, strain MEP2-6T is a promising candidate as a novel biocontrol agent and antibiotic producer.

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Section: Discussionmentioning

confidence: 99%

Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

Wannawong,

Mhuantong,

Macharoen

et al. 2024

Front. Plant Sci.

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“…This YukE might be involved in changing membrane permeability and further lead to iron loss at the early stage of infection. Thus, T7SS is important for niche colonization in mutualistic interactions with host plant ( Liu et al, 2023 ).…”

Section: Secretion System In Pgprs: An Overviewmentioning

confidence: 99%

Secretory molecules from secretion systems fine-tune the host-beneficial bacteria (PGPRs) interaction

Gupta,

Chauhan,

Jha

et al. 2024

Front. Microbiol.

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Numerous bacterial species associate with plants through commensal, mutualistic, or parasitic association, affecting host physiology and health. The mechanism for such association is intricate and involves the secretion of multiple biochemical substances through dedicated protein systems called secretion systems SS. Eleven SS pathways deliver protein factors and enzymes in their immediate environment or host cells, as well as in competing microbial cells in a contact-dependent or independent fashion. These SS are instrumental in competition, initiation of infection, colonization, and establishment of association (positive or negative) with host organisms. The role of SS in infection and pathogenesis has been demonstrated for several phytopathogens, including Agrobacterium, Xanthomonas, Ralstonia, and Pseudomonas. Since there is overlap in mechanisms of establishing association with host plants, several studies have investigated the role of SSs in the interaction of plant and beneficial bacteria, including symbiotic rhizobia and plant growth bacteria (PGPB). Therefore, the present review updates the role of different SSs required for the colonization of beneficial bacteria such as rhizobia, Burkholderia, Pseudomonas, Herbaspirillum, etc., on or inside plants, which can lead to a long-term association. Most SS like T3SS, T4SS, T5SS, and T6SS are required for the antagonistic activity needed to prevent competing microbes, including phytopathogens, ameliorate biotic stress in plants, and produce substances for successful colonization. Others are required for chemotaxis, adherence, niche formation, and suppression of immune response to establish mutualistic association with host plants.

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“…cucumerinum J. H. Owen (FOCO) increased the concentrations of citric acid, fumaric acid, and tryptophan in root exudates (Liu et al, 2014(Liu et al, , 2017. Citric acid acts as a chemoattractant, and fumaric acid serves as a biofilm stimulator for the biocontrol strain B. velezensis SQR9; being the precursor for indole 3-acetic acid (IAA) synthesis, tryptophan can also contribute to enhanced rhizosphere colonization of the IAA-producing strain SQR9 (Liu et al, 2014(Liu et al, , 2017; additionally, the plant commensal type VII secretion system in strain SQR9 causes iron leakage from roots to promote its own colonization and subsequent plant beneficial functions (Liu, Shu, et al, 2023;Liu, Zhao, et al, 2023). Therefore, induced root secretion of different molecular signals can recruit the beneficial rhizobacterium SQR9 to resist FOCO infection (Liu et al, 2014).…”

Section: Pathogen Suppressionmentioning

confidence: 99%

“…Complicated and dynamic factors, such as soil characteristics, plant genotypes, and indigenous microbiota, can all influence the colonization and functional efficacy of inoculated Bacillus agents (Xiong et al, 2017). Accordingly, a few approaches have been attempted to enhance biocontrol efficiency, such as the combination of Bacillus strains with organic amendments to make bioorganic fertilizers (Cao et al, 2011;Chen et al, 2020;Ling et al, 2010;Qiu et al, 2012;Xiong et al, 2017;Zhang et al, 2011), the use of antagonistic metabolites instead of/mixed with biocontrol agents (Kang et al, 2021;Medeot et al, 2017;Mihalache et al, 2018;Ravi et al, 2021), and the combined application of bacterial isolates and chemical agents (e.g., fungicides; Ji et al, 2019;Liu, Shu, et al, 2023;Liu, Zhao, et al, 2023). These strategies have been summarized to improve the biocontrol efficacy of Bacillus agents on different levels, and two emerging strategies, including synthetic microbial consortia and rhizosphere-derived prebiotics, are introduced here (Du et al, 2021; Figure 3).…”

Section: Pr Actical and Emerging Str Ategies For Enhancing The Biocon...mentioning

confidence: 99%

Biocontrol mechanisms of Bacillus: Improving the efficiency of green agriculture

Zhang,

Wang,

Shao

et al. 2023

Microbial Biotechnology

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Species of the genus Bacillus have been widely used for the biocontrol of plant diseases in the demand for sustainable agricultural development. New mechanisms underlying Bacillus biocontrol activity have been revealed with the development of microbiome and microbe‐plant interaction research. In this review, we first briefly introduce the typical Bacillus biocontrol mechanisms, such as the production of antimicrobial compounds, competition for niches/nutrients, and induction of systemic resistance. Then, we discussed in detail the new mechanisms of pathogen quorum sensing interference and reshaping of the soil microbiota. The “cry for help” mechanism was also introduced, in which plants can release specific signals under pathogen attack to recruit biocontrol Bacillus for root colonization against invasion. Finally, two emerging strategies for enhancing the biocontrol efficacy of Bacillus agents, including the construction of synthetic microbial consortia and the application of rhizosphere‐derived prebiotics, were proposed.

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Plant commensal type VII secretion system causes iron leakage from roots to promote colonization

Cited by 30 publications

References 63 publications

Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

Comparative genomics reveals insight into the phylogeny and habitat adaptation of novel Amycolatopsis species, an endophytic actinomycete associated with scab lesions on potato tubers

Secretory molecules from secretion systems fine-tune the host-beneficial bacteria (PGPRs) interaction

Biocontrol mechanisms of Bacillus: Improving the efficiency of green agriculture

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