Nowadays, the development and study of the landscape code (LC) of a botanical garden is relevant to the concept of the formation and development of an institution. In this regard, for the first time, a comprehensive analysis of the territory of the Nikitsky Botanical Gardens (NBG) was carried out using the example of the Upper and Lower parks; their figurative-spatial structure is considered. The work analyzes the influence of abiotic and biotic factors, include and anthropogenic factors on the LC of the NBG arboretum. The results obtained can be used in assessing ancient domestic parks in Russia.