Plant cutin genesis: unanswered questions

Domínguez, Ernesto Redondo; Heredia‐Guerrero, José A.; Heredia, Antonio

doi:10.1016/j.tplants.2015.05.009

Cited by 92 publications

(81 citation statements)

References 79 publications

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“…In Arabidopsis, this putative SUS gene (named here) was found downregulated in both myb107 and myb9 lines. Cutin polymerization has recently received significant attention and yet still remains largely undescribed (Domínguez et al, 2015). While spontaneous polymerization of cutin monomers was considered, CUS proteins (which have been localized to the extracellular region) have been described to act as acyltransferase enzymes able to link cutin monomers (such as monoglycerols) (Yeats et al, 2012(Yeats et al, , 2014.…”

Section: Discussionmentioning

confidence: 99%

MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

et al. 2016

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Suberin, a polymer composed of both aliphatic and aromatic domains, is deposited as a rough matrix upon plant surface damage and during normal growth in the root endodermis, bark, specialized organs (e.g., potato [Solanum tuberosum] tubers), and seed coats. To identify genes associated with the developmental control of suberin deposition, we investigated the chemical composition and transcriptomes of suberized tomato (Solanum lycopersicum) and russet apple (Malus x domestica) fruit surfaces. Consequently, a gene expression signature for suberin polymer assembly was revealed that is highly conserved in angiosperms. Seed permeability assays of knockout mutants corresponding to signature genes revealed regulatory proteins (i.e., AtMYB9 and AtMYB107) required for suberin assembly in the Arabidopsis thaliana seed coat. Seeds of myb107 and myb9 Arabidopsis mutants displayed a significant reduction in suberin monomers and altered levels of other seed coat-associated metabolites. They also exhibited increased permeability, and lower germination capacities under osmotic and salt stress. AtMYB9 and AtMYB107 appear to synchronize the transcriptional induction of aliphatic and aromatic monomer biosynthesis and transport and suberin polymerization in the seed outer integument layer. Collectively, our findings establish a regulatory system controlling developmentally deposited suberin, which likely differs from the one of stressinduced polymer assembly recognized to date.

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Section: Discussionmentioning

confidence: 99%

MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

et al. 2016

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“…Waxes cover or are embedded into the cutin polymer, forming the plant cuticle (Domínguez et al , ). Since we found changes in the epicuticular wax, we focused on the other component of the cuticle, the cutin.…”

Section: Metabolic Changes In the Receptacles Of Famads9‐silenced Linmentioning

confidence: 99%

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Vallarino

Merchante

Sánchez-Sevilla

et al. 2019

Plant Biotechnology Journal

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FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines did not show differences in basic parameters, such as fruit firmness or colour, but exhibited lower Brix values in three of the four independent lines. The gene ontology MapMan category that was most enriched among the differentially expressed genes in the receptacles at the white stage corresponded to the regulation of transcription, including a high percentage of transcription factors and regulatory proteins associated with auxin action. In contrast, the most enriched categories at the red stage were transport, lipid metabolism and cell wall. Metabolomic analysis of the receptacles of the transformed fruits identified significant changes in the content of maltose, galactonic acid‐1,4‐lactone, proanthocyanidins and flavonols at the green/white stage, while isomaltose, anthocyanins and cuticular wax metabolism were the most affected at the red stage. Among the regulatory genes that were differentially expressed in the transgenic receptacles were several genes previously linked to flavonoid metabolism, such as MYB10, DIV, ZFN1, ZFN2, GT2, and GT5, or associated with the action of hormones, such as abscisic acid, SHP, ASR, GTE7 and SnRK2.7. The inference of a gene regulatory network, based on a dynamic Bayesian approach, among the genes differentially expressed in the transgenic receptacles at the white and red stages, identified the genes KAN1, DIV, ZFN2 and GTE7 as putative targets of FaMADS9. A MADS9‐specific CArG box was identified in the promoters of these genes.

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“…Cutin forms an amorphous and viscoelastic framework based on the interesterification of C 16 and C 18 polyhydroxyalkanoic acids (Heredia, 2003;Domínguez et al, 2011). The significant progress made toward understanding cutin chemical composition and its monomer biosynthesis (Heredia, 2003;Pollard et al, 2008;Beisson et al, 2012) has recently been accompanied by a partial description of the mechanism of transport and further synthesis of cutin (Heredia-Guerrero et al, 2008;Girard et al, 2012;Yeats et al, 2012;Domínguez et al, 2015).…”

mentioning

confidence: 99%

Ultrastructure of the Epidermal Cell Wall and Cuticle of Tomato Fruit (Solanum lycopersicum L.) during Development

2015

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The epidermis plays a pivotal role in plant development and interaction with the environment. However, it is still poorly understood, especially its outer epidermal wall: a singular wall covered by a cuticle. Changes in the cuticle and cell wall structures are important to fully understand their functions. In this work, an ultrastructure and immunocytochemical approach was taken to identify changes in the cuticle and the main components of the epidermal cell wall during tomato fruit development. A thin and uniform procuticle was already present before fruit set. During cell division, the inner side of the procuticle showed a globular structure with vesicle-like particles in the cell wall close to the cuticle. Transition between cell division and elongation was accompanied by a dramatic increase in cuticle thickness, which represented more than half of the outer epidermal wall, and the lamellate arrangement of the non-cutinized cell wall. Changes in this non-cutinized outer wall during development showed specific features not shared with other cell walls. The coordinated nature of the changes observed in the cuticle and the epidermal cell wall indicate a deep interaction between these two supramolecular structures. Hence, the cuticle should be interpreted within the context of the outer epidermal wall.

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Plant cutin genesis: unanswered questions

Cited by 92 publications

References 79 publications

MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Ultrastructure of the Epidermal Cell Wall and Cuticle of Tomato Fruit (Solanum lycopersicum L.) during Development

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