Plant defence mechanisms against mycotoxin Fumonisin B1

Iqbal, Nadeem; Czékus, Zalán; Poór, Péter; Ördög, Attila

doi:10.1016/j.cbi.2021.109494

Cited by 28 publications

(26 citation statements)

References 159 publications

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“…At cellular level, fumonisins act as inhibitor of the isoenzyme LONGEVITY ASSURANCE GENE ONE HOMOLOG1 (LOH1), leading to the accumulation of specific long chain bases (LCB) that induce a SA-dependent cell death response (Luttegeharm et al 2015(Luttegeharm et al , 2016. The imbalance in LCB, together with the induction of oxidative stress at cytoplasm and ER level, triggers different downstream signaling pathways, eventually leading to the programmed cell death (PCD) in plants (Qin et al 2017;Iqbal et al 2021). Conversely, the overexpression of LOH1 did not disclose any resistance to the mycotoxin (Luttegeharm et al 2015(Luttegeharm et al , 2016.…”

Section: Introductionmentioning

confidence: 99%

“…However, the exact mechanism behind the antioxidant defense system and crosstalk among the phytohormones, sphingolipids ratio, and the resulting responses at nuclear and organelle level that led to PCD is still unclear. Likewise, it remains to be established whether the oxidative stress is caused by FB 1 or a consequence of other events that take place upon exposure (Zeng et al 2020;Iqbal et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Arabidopsis thaliana is a model organism, which has been extensively used to study gene expression and toxicity mechanisms induced by FB 1 exposure at cellular level (Iqbal et al 2021). Understanding the molecular and biochemical pathways induced by this mycotoxin is essential to counteract the toxic effects of fungal and mycotoxin contamination, and possibly to identify bioactive compounds able to minimize or neutralize them or key components that confer resistance to mycotoxin or fungal contamination.…”

Section: Introductionmentioning

confidence: 99%

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Cell death induced by mycotoxin fumonisin B1 is accompanied by oxidative stress and transcriptional modulation in Arabidopsis cell culture

et al. 2022

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Key message Fumonisin B1 induces rapid programmed cell death in Arabidopsis cells, oxidative and nitrosative bursts, and differentially modulates cell death responsive genes. Glutathione is the main antioxidant involved in the stress response. Abstract Fumonisin B1 (FB1) is a fungal toxin produced by Fusarium spp. able to exert pleiotropic toxicity in plants. FB1 is known to be a strong inducer of the programmed cell death (PCD); however, the exact mechanism underling the plant–toxin interactions and the molecular events that lead to PCD are still unclear. Therefore, in this work, we provided a comprehensive investigation of the response of the model organism Arabidopsis thaliana at the nuclear, transcriptional, and biochemical level after the treatment with FB1 at two different concentrations, namely 1 and 5 µM during a time-course of 96 h. FB1 induced oxidative and nitrosative bursts and a rapid cell death in Arabidopsis cell cultures, which resembled a HR-like PCD event. Different genes involved in the regulation of PCD, antioxidant metabolism, photosynthesis, pathogenesis, and sugar transport were upregulated, especially during the late treatment time and with higher FB1 concentration. Among the antioxidant enzymes and compounds studied, only glutathione appeared to be highly induced in both treatments, suggesting that it might be an important stress molecule induced during FB1 exposure. Collectively, these findings highlight the complexity of the signaling network of A. thaliana and provide information for the understanding of the physiological, molecular, and biochemical responses to counteract FB1-induced toxicity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell death induced by mycotoxin fumonisin B1 is accompanied by oxidative stress and transcriptional modulation in Arabidopsis cell culture

et al. 2022

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“…At cellular level, fumonisins act as competitive substrates and inhibitors of the enzyme ceramide synthase, causing an accumulation of sphinganine and sphingosine, the natural ceramide synthase substrates, and a depletion of dihydro-sphingosine, complex sphingolipids, and ceramides in the endoplasmic reticulum (ER). This imbalance, together with the induction of oxidative stress at cytoplasm and ER level, triggers different downstream signaling pathways and epigenetic changes, eventually leading to the programmed cell death (PCD) in plants (Qin et al, 2017;Iqbal et al, 2021). Besides being structural components of the cell membrane, sphingolipids act as secondary messengers and regulate the expression of the phytohormones salicylic acid (SA), jasmonic acid (JA) and ethylene (ET).…”

Section: Introductionmentioning

confidence: 99%

“…No signi cant variation was displayed by the APX and CRN1 genes for both treatment times and concentrations, except CRN1 at 48 h that resulted signi cantly more expressed under 5 µM FB 1 exposure (Fig4 f).It is known that ethylene (ET) is involved in plant responses to FB 1 and contributes to PCD and activation of defense mechanisms by a concentration and time-dependent manner(Zeng et al, 2020;Iqbal et al, 2021). Different phenotypes were observed in the Arabidopsis ethylene response 1-1 (etr1-1) mutants, probably due to the diverse light and growth conditions(Asai et al, 2000;Iqbal et al, 2021). Wu and coworkers (2015) by employing several ET mutants reported that sphingolipid synthesis was suppressed by ET signaling that acted as a negative regulator of FB 1 -challenged PCD Moore et al (1999).…”

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confidence: 99%

The Mycotoxin Fumonisin B1 Induces Metabolic and Molecular Perturbations Leading to Cell Death in Arabidopsis Cell Culture

Lanubile

Michele

Loi

et al. 2022

Preprint

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Fumonisin B1 (FB1) is a fungal toxin produced by Fusarium spp. able to exert pleiotropic toxicity in plants. FB1 is known to be a strong inducer of the programmed cell death (PCD); however, the exact mechanism underling the plant-toxin interactions and the molecular events that lead to PCD are still unclear. Therefore, in this work we provided a comprehensive investigation of the response of the model organism Arabidopsis thaliana at the nuclear, transcriptional, and biochemical level after the treatment with FB1 at two different concentrations, namely 1 and 5µM during a time-course of 96 h. FB1 induced oxidative and nitrosative bursts and a rapid cell death in Arabidopsis cell cultures, which resembled a HR-like PCD event. Different genes involved in the regulation of PCD, antioxidant metabolism, photosynthesis, resistance, and sugar transport were upregulated, especially during the late treatment time and with higher FB1 concentration. Among the antioxidant enzymes and compounds studied, only glutathione appeared to be highly induced in both treatments, suggesting that it might be an important defense molecule induced during FB1 exposure. Collectively, these findings highlight the complexity of the defense network of A. thaliana and provide important information for the understanding of the physiological, molecular, and biochemical responses to counteract FB1-induced toxicity.

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Fusaric acid-evoked oxidative stress affects plant defence system by inducing biochemical changes at subcellular level

Iqbal,

Czékus,

Ördög

et al. 2023

Plant Cell Rep

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Fusaric acid (FA) is one of the most harmful phytotoxins produced in various plant–pathogen interactions. Fusarium species produce FA as a secondary metabolite, which can infect many agronomic crops at all stages of development from seed to fruit, and FA production can further compromise plant survival because of its phytotoxic effects. FA exposure in plant species adversely affects plant growth, development and crop yield. FA exposure in plants leads to the generation of reactive oxygen species (ROS), which cause cellular damage and ultimately cell death. Therefore, FA-induced ROS accumulation in plants has been a topic of interest for many researchers to understand the plant–pathogen interactions and plant defence responses. In this study, we reviewed the FA-mediated oxidative stress and ROS-induced defence responses of antioxidants, as well as hormonal signalling in plants. The effects of FA phytotoxicity on lipid peroxidation, physiological changes and ultrastructural changes at cellular and subcellular levels were reported. Additionally, DNA damage, cell death and adverse effects on photosynthesis have been explained. Some possible approaches to overcome the harmful effects of FA in plants were also discussed. It is concluded that FA-induced ROS affect the enzymatic and non-enzymatic antioxidant system regulated by phytohormones. The effects of FA are also associated with other photosynthetic, ultrastructural and genotoxic modifications in plants. Graphical abstract

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Plant defence mechanisms against mycotoxin Fumonisin B1

Cited by 28 publications

References 159 publications

Cell death induced by mycotoxin fumonisin B1 is accompanied by oxidative stress and transcriptional modulation in Arabidopsis cell culture

Cell death induced by mycotoxin fumonisin B1 is accompanied by oxidative stress and transcriptional modulation in Arabidopsis cell culture

The Mycotoxin Fumonisin B1 Induces Metabolic and Molecular Perturbations Leading to Cell Death in Arabidopsis Cell Culture

Fusaric acid-evoked oxidative stress affects plant defence system by inducing biochemical changes at subcellular level

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