Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Swarupa, V.; Ravishankar, K. V.; Rekha, A.

doi:10.1007/s00425-013-2024-8

Cited by 96 publications

(69 citation statements)

References 143 publications

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“…Fusarium oxysporum is a widespread necrotrophic fungus that affects several crops. The fungus infection uses an oxidative burst that serves as a weapon; it triggers nutrient leakage from the host cells allowing the pathogen to survive from dead tissues causing significant crop losses [21]. In the present work, we observed that transgenic plants of tobacco showed different expression levels of OxDC gene, which might be associated to different fungus tolerance levels.…”

Section: 23rt-pcr Oxdc Expression Analysis In T 1 Transgenic Lineagessupporting

confidence: 49%

Control of Fusarium oxysporum infection in transgenic tobacco carrying oxalate descarboxilase gene.

Amaral¹,

Pinto²,

Souza³

et al. 2017

J App Biol Biotech

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Fusarium oxysporum is a widespread necrotrophic plant pathogen. Its infection affects several crop plants such as potato, tomato, cotton, banana, coffee, strawberry and sugarcane. The fungus produces oxalic acid, which triggers apoptosis in plants by a reactive oxygen species-dependent mechanism. Tobacco plants were genetically modified to express a gene coding for the enzyme oxalate descarboxilase (OxDc), isolated from Flammulina velutipes. The transgenic plants showed tolerance to Fusarium oxysporum. Even under lower expression of the gene, plants were able to control F. oxysporum infection. Thus, we demonstrated that the expression of the oxdc gene is a good alternative for the development of F. oxysporum resistant crops.

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Section: 23rt-pcr Oxdc Expression Analysis In T 1 Transgenic Lineagessupporting

confidence: 49%

Control of Fusarium oxysporum infection in transgenic tobacco carrying oxalate descarboxilase gene.

Amaral¹,

Pinto²,

Souza³

et al. 2017

J App Biol Biotech

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“…ROS can be involved in HR to produce cell death, in crosslinking with glycoproteins to reinforce the cell wall, or couple with other signaling factors to induce SAR (Torres et al, 2006; Xiao et al, 2013; Swarupa et al, 2014). The first step in ROS production is mediated by NADPH oxidase/RBO (Torres et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Galindo‐González

Deyholos

2016

Front. Plant Sci.

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Fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. Along with rust, fusarium wilt has become an important factor in flax production worldwide. Resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. This interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform breeding programs on potential candidate genes to evaluate or improve resistance across cultivars. In the current study, disease progression in two flax cultivars [Crop Development Center (CDC) Bethune and Lutea], showed earlier disease symptoms and higher susceptibility in the later cultivar. Chitinase gene expression was also divergent and demonstrated and earlier molecular response in Lutea. The most resistant cultivar (CDC Bethune) was used for a full RNA-seq transcriptome study through a time course at 2, 4, 8, and 18 days post-inoculation (DPI). While over 100 genes were significantly differentially expressed at both 4 and 8 DPI, the broadest deployment of plant defense responses was evident at 18 DPI with transcripts of more than 1,000 genes responding to the treatment. These genes evidenced a reception and transduction of pathogen signals, a large transcriptional reprogramming, induction of hormone signaling, activation of pathogenesis-related genes, and changes in secondary metabolism. Among these, several key genes that consistently appear in studies of plant-pathogen interactions, had increased transcript abundance in our study, and constitute suitable candidates for resistance breeding programs. These included: an induced RPMI-induced protein kinase; transcription factors WRKY3, WRKY70, WRKY75, MYB113, and MYB108; the ethylene response factors ERF1 and ERF14; two genes involved in auxin/glucosinolate precursor synthesis (CYP79B2 and CYP79B3); the flavonoid-related enzymes chalcone synthase, dihydroflavonol reductase and multiple anthocyanidin synthases; and a peroxidase implicated in lignin formation (PRX52). Additionally, regulation of some genes indicated potential pathogen manipulation to facilitate infection; these included four disease resistance proteins that were repressed, indole acetic acid amido/amino hydrolases which were upregulated, activated expansins and glucanases, amino acid transporters and aquaporins, and finally, repression of major latex proteins.

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“…These increases in the control treatments occurred two weeks after the increase in the group of plants treated with 1 and 2% EA. Increases in SA content were between 2 and 5 times higher than the basal values reported in plants at the onset of flowering, and in plants infected with necrotic pathogens such as Fusarium oxysporum (Swarupa et al, 2014). This fact may explain the increase in SA content in the control plants from week five to seven.…”

Section: Cuantificación De áCido Salicílico (As)mentioning

confidence: 72%

“…Estos incrementos en los controles, se presentaron dos semanas posterior al incremento que se presentó en el grupo de plantas tratadas con el EA al 1 y 2%. Los incrementos en el contenido de AS entre 2 a 5 veces los valores basales, son reportados en plantas durante el inicio de la floración, así como en plantas infectadas con patógenos necróticos como Fusarium oxysporum (Swarupa et al, 2014). Pudiendo ser esta la razón de los incrementos del AS en las plantas control en las semanas de la quinta a la séptima.…”

Section: Fully Bilingualunclassified

Inducción de respuesta de defensa en plantas de tomate contra Forl por extracto de ajo

Aguilar-Gastélum

Martínez-Téllez

Corrales-Maldonado

et al. 2018

RMF

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Resumen. Se evaluó un extracto de ajo (EA) para inducir la defensa de plantas de tomate contra Fusarium oxysporum radicis-lycopersici (Forl), mediante el incremento de la concentración endógena de ácido salicílico (AS) y ácido jasmónico (AJ). La aspersión de 1 y 2 % del EA sobre plantas intencionalmente infectadas con 1x10 7 esporas mL -1 de Forl, redujeron en 73.53% la severidad de la enfermedad producida por Forl respecto al control infectado. La altura fue similar (p≤0.05) en plantas tratadas con EA y no-tratadas. Abstract. A garlic extract (EA) was evaluated to induce the defense of tomato plants againstFusarium oxysporum radicis-lycopersici (Forl), by increasing the endogenous concentration of salicylic acid (AS) and jasmonic acid (AJ). Spraying 1 and 2% of EA on plants intentionally infected with 1x10 7 spores.mL -1 of Forl reduced in 73.53% the severity of the disease produced by Forl with respect to the infected control. The height was similar (p≤0.05) in plants treated with EA and untreated. The accumulation of AS in plants spayed with 1 and 2% of EA presented two significant increases (p≤0.05), in the second and fifth weeks after spraying, with higher concentrations than the infected and non-infected controls in 11 and 6 times for the first and 2.7 times and 3.8 times for the second, respectively. The AJ was induced from the first week after the treatments, increasing 2.5 and 1.8 times more than the infected and non-infected controls, maintaining those values throughout the evaluation. The results show that the EA reduced the development of the disease caused by Forl Publicación en línea, septiembre 2018 395Fully Bilingual Revista Mexicana de FITOPATOLOGÍA Mexican Journal of Phytopathology

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Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

Abstract: breeding, transgenic and cisgenic approaches have been discussed. This would help us to understand host defenses against Fusarium oxysporum and to formulate strategies to develop tolerant cultivars.

Cited by 96 publications

References 143 publications

Control of Fusarium oxysporum infection in transgenic tobacco carrying oxalate descarboxilase gene.

Control of Fusarium oxysporum infection in transgenic tobacco carrying oxalate descarboxilase gene.

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Inducción de respuesta de defensa en plantas de tomate contra Forl por extracto de ajo

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