Plant derived antioxidants and antifibrotic drugs: past, present and future

Ezhilarasan, Devaraj; Sokal, Étienne; Karthikeyan, S.; Najimi, Mustapha; Khan, Shah Alam

doi:10.12980/jclm.2.2014apjtb-2014-0111

Cited by 33 publications

(22 citation statements)

References 48 publications

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“…In traditional medicinal systems, herbal products have been used to treat myriad ailments, including symptoms associated with cancer . Herbal medicines possess effective anticancer compounds that may be used as adjuvants along with existing chemotherapeutic drugs to improve efficacy and/or reduce drug‐induced toxicity .…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic potentials of S. cumini methanolic seed kernel extract in human hepatoma HepG2 cells

Prakash

Ezhilarasan

2019

Environmental Toxicology

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Syzygium cumini (Myrtaceae) is commonly called as Jamun or Jambolan. It has antidiabetic, anti‐inflammatory, antipyretic, and antioxidant activities. Hepatocellular carcinoma is the most frequent and deadliest cancers worldwide. We investigated the cytotoxic potentials of S. cumini methanolic seed kernel extract against human hepatoma HepG2 cells. HepG2 cells were treated with 10, 20, and 40 μg/mL of seed kernel extract for 24 hours and cytotoxic analysis was performed by MTT assay. S. cumini induced apoptosis related morphological changes in HepG2 cells were analyzed by annexin V and propidium iodide double staining. Nuclear fragmentation and chromatin condensation were analyzed by Hoechst nuclear staining. Mitochondrial membrane potential (MMP) was investigated by 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) staining. Protein expressions of hepatocyte nuclear factor‐1α (HFN‐1α) was performed using western blotting. S. cumini treatments caused a significant and a concentration‐dependent increase in the cytotoxicity of HepG2 cells. S. cumini treatments increased the percentage of cells in an early and late apoptosis stage. This treatment also caused chromatin condensation and nuclear fragmentation. Further, S. cumini treatments decreased MMP and also caused a significant downregulation of HFN‐1α protein expression. The present study demonstrated that S. cumini seed extract induced apoptosis in HepG2 cells through decrease in MMP and downregulation of HFN‐1α.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic potentials of S. cumini methanolic seed kernel extract in human hepatoma HepG2 cells

Prakash

Ezhilarasan

2019

Environmental Toxicology

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“…The plant-based drug discovery resulted primarily in the development of antioxidant, anticancer, and other anti-infectious agents and continues to contribute to the new leads in clinical trials. [ 27 ] Plant-based drug therapy is a new therapeutic modality which targets specific molecules in the cancer cell and modulates signaling pathways involved in carcinogenesis. [ 28 ] A. catechu has been extensively studied for its myriad beneficial effects against various etiologies.…”

Section: Discussionmentioning

confidence: 99%

Acacia catechu ethanolic seed extract triggers apoptosis of SCC-25 cells

Thangavelu¹,

Ezhilarasan²,

Nagaich³

et al. 2017

Phcog Mag

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Background:Acacia catechu Willd (Fabaceae), commonly known as catechu, cachou, and black cutch, has been studied for its hepatoprotective, antipyretic, antidiarrheal, hypoglycemic, anti-inflammatory, immunomodulatory, antinociceptive, antimicrobial, free radical scavenging, and antioxidant activities.Objective:We evaluated the cytotoxic activity of ethanol extract of A. catechu seed (ACS) against SCC-25 human oral squamous carcinoma cell line.Methods:Cytotoxic effect of ACS extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, using concentrations of 0.1–1000 μg/mL for 24 h. A. catechu ethanol seed extract was treated SCC-25 cells with 25 and 50 μg/mL. At the end of treatment period, apoptotic marker gene expressions such as caspase 8, 9, Bcl-2, Bax, and cytochrome c were evaluated by semiquantitative reverse transcription-polymerase chain reaction. Morphological changes of ACS treated SCC-25 cells was evaluated by acridine orange/ethidium bromide (AO/EB) dual staining. Nuclear morphology and DNA fragmentation was evaluated by propidium iodide (PI) staining.Results:A. catechu ethanol seed extract treatment caused cytotoxicity in SCC-25 cells with an IC50 value of 100 μg/mL. Apoptotic markers caspases 8 and 9, cytochrome c, Bax gene expressions were significantly increased upon ACS extract treatment indicate the apoptosis induction in SCC-25 cells. This treatment also caused significant downregulation of Bcl-2 gene expression. Staining with AO/EB and PI shows membrane blebbing, and nuclear membrane distortion further confirms the apoptosis induction by ACS treatment in SCC-25 cells.Conclusion:The ethanol seed extracts of A. catechu was found to be cytotoxic at lower concentrations and induced apoptosis in human oral squamous carcinoma SCC-25 cells.SUMMARY Acacia catechu ethanolic seed extract contains phytochemicals such as epicatechin, rutin, and quercetinAcacia catechu seed (ACS) extract significantly (P < 0.001) inhibits the active proliferation of human oral squamous carcinoma (SCC-25) cellsACS extract treatment to SCC-25 cells significantly modulated the gene expressions pertaining to apoptosis and propidium iodide and acridine orange/ethidium bromide staining also confirm the apoptosis inductionAntiproliferative and apoptosis inducing activities of ACS extract is correlated with phytochemical contents. Abbreviations used: ACS: Acacia catechu seed extract; MTT: 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; AO/EO: Acridine orange/ethidium bromide; LC MS: Liquid chromatography mass spectrometry.

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“…Drugs derived from natural sources play a significant role in the prevention and treatment of human diseases. 12 Hence, in this study, we tested the antifungal effect of hesperetin and silibinin against different fungal species. Silibinin (SBN) is a flavonolignan extracted from the seeds of a medicinal plant Silybum marianum (Asteraceae), commonly called "milk thistle".…”

Section: Introductionmentioning

confidence: 99%

In vitro Antifungal Effects of Hesperetin and Silibinin

Gowtham¹,

Yousuf²,

Ezhilarasan³

et al. 2018

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Background:Increasing resistance of microorganisms against available antimicrobial agents is of major concern among scientists and clinicians worldwide. To overcome the resistance and other drawbacks of the current antimicrobial drugs and to obtain more efficacious drugs, an antimicrobial drug having a novel mode of action should be developed. The flavonoid compounds have demonstrated for their therapeutic potential as antimicrobial agents previously. Aim: To evaluate the antifungal efficacy of plant derived compounds such as hesperetin (HSP) and silibinin (SBN) compounds in vitro. Materials and Methods: In this study, we evaluated the antifungal effect of two plant derived flavonoid compounds i.e., SBN and HSP against Candida albicans, Candida tropicalis, Malassezia furfur, Aspergillus niger, Penicillium oxalicum. The disk diffusion test was performed to test the antifungal effect of HSP and SBN with three different concentrations i.e., 15, 20, 25 mg/ml of HSP and SBN. The antifungal efficacy was studied for 72 h. Results: Our results indicate that SBN has significant antifungal effect than that of HSP. The high sensitivity of these compounds was found against C. albicans, C. tropicalis. Conclusion: These drugs could be good candidate to combine with regular antimicrobial agents.

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Plant derived antioxidants and antifibrotic drugs: past, present and future

Cited by 33 publications

References 48 publications

Cytotoxic potentials of S. cumini methanolic seed kernel extract in human hepatoma HepG2 cells

Cytotoxic potentials of S. cumini methanolic seed kernel extract in human hepatoma HepG2 cells

Acacia catechu ethanolic seed extract triggers apoptosis of SCC-25 cells

In vitro Antifungal Effects of Hesperetin and Silibinin

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