Plant-derived Ca, N, S-Doped carbon dots for fast universal cell imaging and intracellular Congo red detection

Durrani, Samran; Zhang, Jie; Yang, Zihuayuan; Pang, Aiping; Zeng, Jia; Sayed, Sayed Mir; Khan, Adeel; Zhang, Yaqin; Wu, Fugen; Lin, Fengming

doi:10.1016/j.aca.2022.339672

Cited by 24 publications

(16 citation statements)

References 58 publications

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“…Obviously, the fluorescence emission spectrum of GB-CDs was dependent on the excitation wavelength. The relative quantum yield (QY) of GB-CDs was calculated to be 7.2%, which was not that high as compared to that of some CDs reported in the literature which exhibited a QY of 20–70% but is comparable with that of other reported CDs. , Some CDs without proper surface passivation often have QY less than 10% . Sometimes, QY is still too low even after surface functionalization/doping.…”

Section: Resultsmentioning

confidence: 73%

“…Fluorescent sensors selectively targeting subcellular organelles are powerful tools in the fields of biochemistry, molecular biology, and clinical diagnostics because subcellular localization allows selective monitoring of biological events occurring in a specific region with high accuracy . Carbon dots (CDs) as a type of quasi-spherical carbon-based fluorescent nanomaterials in amorphous or nanocrystalline structure have recently found tremendous application potential in areas including bioimaging, sensors, catalysis, biomedicine, fluorescent ink, and optoelectronics. − Compared to organic fluorophores or conventional semiconductor quantum dots, CDs have appealing merits including ease of synthesis, facile functionalization, unique photoelectronic properties, chemical inertness, and low toxicity, placing them in an advantageous position for achieving unprecedented performance . Currently, most existing CD-based fluorescence biosensors detect cellular Fe 3+ , and adenosine triphosphate (ATP) , in the whole living cells.…”

Section: Introductionmentioning

confidence: 99%

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Biomass-Based Carbon Dots for Fe³⁺ and Adenosine Triphosphate Detection in Mitochondria

Durrani

Zhang

Mukramin

et al. 2022

ACS Appl. Nano Mater.

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Real-time live-cell monitoring of Fe3+ and adenosine triphosphate (ATP) in mitochondria important for cellular functions and related to different diseases is highly desired, yet lack study. Herein, carbon dots, green bean (GB)-carbon dots (CDs), were made from GB through easy one-step hydrothermal treatment. GB-CDs were in spherical form with a diameter of 13 ± 3 nm, negatively charged, and green emissive. GB-CDs contained amino groups, which enabled their binding to Fe3+, resulting in their fluorescence quenching. Based on this, GB-CDs were employed to detect Fe3+ with high selectivity, showing a linear detection range of 10–70 μM and a detection limit of 3.6 nM. Furthermore, the addition of ATP can recover the fluorescence quenching of GB-CDs by Fe3+. Therefore, the GB-CD + Fe3+ system was deployed to assay ATP with a linear detection range of 50–600 μM and a detection limit of 60 nM, exhibiting high sensitivity and specificity. GB-CDs and GB-CDs + Fe3+ were individually harnessed to assess Fe3+ in tap water and ATP in fetal bovine serum. More importantly, GB-CDs can enter cells to localize in mitochondria for mitochondrial imaging with high biocompatibility and superior photostability. As a result, GB-CDs and GB-CD + Fe3+ system were implemented to track Fe3+ and ATP in mitochondria of A549, respectively. This study provides a potent tool to monitor Fe3+ and ATP in mitochondria, expanding the application of CDs as biosensors for biological compounds in subcellular organelles.

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Section: Resultsmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Biomass-Based Carbon Dots for Fe³⁺ and Adenosine Triphosphate Detection in Mitochondria

Durrani

Zhang

Mukramin

et al. 2022

ACS Appl. Nano Mater.

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“…Our group synthesized Ca‐, N‐, S‐doped CDs (Mis‐mPD‐CDs) from plant Salvadora persica and m ‐phenylenediamine (mPD) by a hydrothermal approach. [ 285 ] Mis‐mPD‐CDs were negatively charged and green‐emissive. Because their fluorescence could be quenched by CR through the IFE, Mis‐mPD‐CDs could be used to detect CR with a linear range of 0.2–1.2 μM and an LOD of 58 nM.…”

Section: For Detecting Intracellular Compoundsmentioning

confidence: 99%

Carbon Dots for Intracellular Sensing

Lin

Jia

2022

Small Structures

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Carbon dots (CDs), a type of small, carbon element‐based nanomaterials, have found numerous applications in many fields due to their outstanding properties like fascinating photoluminescence characteristic, great biocompatibility, easy and economical synthesis, and facile functionalization. Over the last decade, huge progress and achievements have been made in terms of the applications of CDs in cellular detection. However, a comprehensive review focusing on this topic is still lacking. Herein, the recent progress in CDs‐mediated intracellular detection, including the fluorescence imaging of cellular structures (e.g., nucleus/nucleolus, mitochondrion, lysosome, endoplasmic reticulum, Golgi apparatus, and lipid droplet), the fluorescence detection of a large variety of cellular substances (including endogenous biomacromolecules and their monomers, vitamins, important metabolites, adenosine triphosphate, reactive oxygen species, biothiols, ions, and exogenous compounds), and the monitoring of cellular parameters (pH, temperature, and mitochondrial membrane potential), is thoroughly reviewed. The synthesis, functionalization, and performance of the CDs that serve as fluorescent nanoprobes in living cells are presented. Their underlying working mechanisms are also discussed if available. Finally, the challenges and future research directions of the related research field are listed. This review may foster the future development of more CDs with better cellular sensing performance.

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“…The Mis-mPD-CDs were used to detect and image CR in living cells ( C. albicans , A549, S. aureus , and E. coli ) and zebrafish. Mis-mPD-CDs were proved to be useful for the quantitative sensing of CR in real samples of industrial wastewater and fish tissues, as well [ 163 ].…”

Section: Applications Of Cdsmentioning

confidence: 99%

“… Synthesis of Mis-mPD-CD and their application for cell-imaging and intracellular CR sensing (reprinted with permission from [ 163 ], 2022, Elsevier). …”

Section: Figurementioning

confidence: 99%

Recent Advances in Synthesis, Modification, Characterization, and Applications of Carbon Dots

Pundi

Chang

2022

Polymers

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Although there is significant progress in the research of carbon dots (CDs), some challenges such as difficulty in large-scale synthesis, complicated purification, low quantum yield, ambiguity in structure-property correlation, electronic structures, and photophysics are still major obstacles that hinder the commercial use of CDs. Recent advances in synthesis, modification, characterization, and applications of CDs are summarized in this review. We illustrate some examples to correlate process parameters, structures, compositions, properties, and performances of CDs-based materials. The advances in the synthesis approach, purification methods, and modification/doping methods for the synthesis of CDs are also presented. Moreover, some examples of the kilogram-scale fabrication of CDs are given. The properties and performance of CDs can be tuned by some synthesis parameters, such as the incubation time and precursor ratio, the laser pulse width, and the average molar mass of the polymeric precursor. Surface passivation also has a significant influence on the particle sizes of CDs. Moreover, some factors affect the properties and performance of CDs, such as the polarity-sensitive fluorescence effect and concentration-dependent multicolor luminescence, together with the size and surface states of CDs. The synchrotron near-edge X-ray absorption fine structure (NEXAFS) test has been proved to be a useful tool to explore the correlation among structural features, photophysics, and emission performance of CDs. Recent advances of CDs in bioimaging, sensing, therapy, energy, fertilizer, separation, security authentication, food packing, flame retardant, and co-catalyst for environmental remediation applications were reviewed in this article. Furthermore, the roles of CDs, doped CDs, and their composites in these applications were also demonstrated.

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Plant-derived Ca, N, S-Doped carbon dots for fast universal cell imaging and intracellular Congo red detection

Cited by 24 publications

References 58 publications

Biomass-Based Carbon Dots for Fe³⁺ and Adenosine Triphosphate Detection in Mitochondria

Biomass-Based Carbon Dots for Fe³⁺ and Adenosine Triphosphate Detection in Mitochondria

Carbon Dots for Intracellular Sensing

Recent Advances in Synthesis, Modification, Characterization, and Applications of Carbon Dots

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Plant-derived Ca, N, S-Doped carbon dots for fast universal cell imaging and intracellular Congo red detection

Cited by 24 publications

References 58 publications

Biomass-Based Carbon Dots for Fe3+ and Adenosine Triphosphate Detection in Mitochondria

Biomass-Based Carbon Dots for Fe3+ and Adenosine Triphosphate Detection in Mitochondria

Carbon Dots for Intracellular Sensing

Recent Advances in Synthesis, Modification, Characterization, and Applications of Carbon Dots

Contact Info

Product

Resources

About

Biomass-Based Carbon Dots for Fe³⁺ and Adenosine Triphosphate Detection in Mitochondria

Biomass-Based Carbon Dots for Fe³⁺ and Adenosine Triphosphate Detection in Mitochondria