Plant-Derived Lactobacillus paracasei IJH-SONE68 Improves Chronic Allergy Status: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

Abstract

We have recently demonstrated that the exopolysaccharides (EPSs) produced by a plant-derived lactic acid bacterium, Lactobacillus paracasei IJH-SONE68, prevent and ameliorate allergic reaction on contact in dermatitis model mice. In the present study, we conducted a clinical trial using a capsule containing spray-dried powder from pineapple juice broth fermented with the LAB strain as an experimental diet. The clinical trial was conducted as a double-blind and placebo-controlled randomized comparative study from May 2019 to July 2021. Males and females between the ages of 21 and 70 who experience chronic allergies participated in the study. Sixty subjects were instructed to orally take a capsule containing the IJH-SONE68 powder or placebo, every day for 12 weeks. After the clinical trial was over, the scores based on subjects’ self-assessment of allergic status were significantly improved in the intervention group, as compared with the placebo group. Some serum biochemicals associated with inflammation response were also significantly improved by intake of the experimental diet. In conclusion, the IJH-SONE68-derived EPS improves chronic allergy status in humans and is expected to decrease their inconvenience.

Construction of a Temperature-Sensitive Shuttle Vector between Lactic Acid Bacteria and <i>Escherichia coli</i> and Its Application to a Tn<i>10</i>-Based Random Mutagenesis Tool in Lactic Acid Bacteria

“…38,56) Therefore, pLES-Tn10-TS1 may also be expected to be a useful plasmid that will be applied for functional and genetic analyses in health-promoting LAB strains with a focus on our isolates confirmed to be useful in randomized controlled trials. [57][58][59][60]…”

Plant-Derived Lactobacillus paracasei IJH-SONE68 Improves the Gut Microbiota Associated with Hepatic Disorders: A Randomized, Double-Blind, and Placebo-Controlled Clinical Trial

“…Interestingly, although biochemical measurements were neither primary nor secondary outcomes, and were monitored only to detect adverse effects, the recent study using the same sample and placebo [ 14 ] also revealed that serum levels of AST and ALT were significantly decreased by IJH-SONE68 intake ( p = 0.001 and 0.022, respectively). Considering the previous and present results together, IJH-SONE68-derived powder seems to have beneficial effects on both parameters.…”

“…It is of more interest that the relative abundance of the Anaerostipes species has reportedly decreased in infants and children suffering from food or respiratory allergies and eczema [ 35 , 36 , 37 , 38 , 39 ]. Although a gut microbiota analysis was not performed in our recent clinical study on allergic symptoms [ 14 ], it is expected that similar changes may also have occurred in the subjects, suggesting that an increase in Anaerostipes contributed to the improvement in hepatic parameters and allergic status.…”

“…This was achieved via the reduction in the accelerated expression of inflammatory cytokines [ 12 , 13 ]. Our clinical study involving volunteer participants with perennial allergy symptoms also showed that the EPS from the IJH-SONE68 strain improved allergic conditions [ 14 ].…”

Lactobacillus paracaseiAH2 isolated from Chinese sourdough alleviated gluten‐induced food allergy through modulating gut microbiota and promoting short‐chain fatty acid accumulation in a BALB/c mouse model

“…With the function of ameliorating gut microbiota and regulating short‐chain fatty acid (SCFA) metabolism, L. paracasei has been intensively explored and shown to have multiple beneficial anti‐inflammation effects on allergic rhinitis, pollen allergy, β ‐lactoglobulin allergy, as well as chronic allergy status 14 . As investigated in our previous research, 15 L. paracasei AH2 isolated from traditionally homemade sourdough in Anhui province of China showed a potential to reduce the immune reactivity of wheat protein by in vitro evaluation.…”