Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following &lt;em&gt;Porphyromonas gingivalis&lt;/em&gt; infection

Meresta, Anna; Folkert, Justyna; Gaber, Timo; Miksch, K.; Buttgereit, Frank; Detert, J.; Pischon, Nicole; Gurzawska, Katarzyna

doi:10.2147/ijn.s113740

Cited by 12 publications

(11 citation statements)

References 44 publications

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“…Furthermore, the RG‐I molecules are not enzymatically degraded in the body and their structure is relatively easy to control . Our results showed that the RG‐I properties may have a great effect not only on osteoblasts, but also on fibroblasts during the bone healing process due to increasing expression levels of COL1A1 and FN1 (Fig. ).…”

Section: Discussionmentioning

confidence: 71%

“…[8][9][10][11] Recently, it was reported that pectin polysaccharide rhamnogalacturonan-I (RG-I) can prevent inflammation, 8,9 and improve tissue regeneration. [12][13][14][15][16] Our previous studies also showed that nanocoated RG-I pectins enhance bone tissue regeneration in vitro through upregulation of the gene expression of osteogenic markers in human and murine osteoblasts. [17][18][19] Moreover, pectin polysaccharides have been reported to affect the behavior of fibroblasts, the most common cells of connective tissue, which play a critical role in the soft and bone tissue healing process.…”

Section: Introductionmentioning

confidence: 86%

“…The chemical properties, monosaccharide composition, and linkage analysis of PU and PA have been presented in our previous works . In this study, PU and PA RG‐Is (128 µg mL −1 ) were adhered to the surface of 6‐well, 24‐well, and 96‐well tissue culture polystyrene (TCPS) plates (Techno Plastic Product) as described earlier …”

Section: Methodsmentioning

confidence: 99%

“…18,19 In this study, PU and PA RG-Is (128 mg mL 21 ) were adhered to the surface of 6-well, 24-well, and 96-well tissue culture polystyrene (TCPS) plates (Techno Plastic Product) as described earlier. 14…”

Section: Methodsmentioning

confidence: 99%

“…A number of studies have shown that plant‐derived polysaccharides are immunomodulatory agents . Recently, it was reported that pectin polysaccharide rhamnogalacturonan‐I (RG‐I) can prevent inflammation, and improve tissue regeneration . Our previous studies also showed that nanocoated RG‐I pectins enhance bone tissue regeneration in vitro through upregulation of the gene expression of osteogenic markers in human and murine osteoblasts .…”

Section: Introductionmentioning

confidence: 99%

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Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

Mieszkowska

Folkert

Gaber

et al. 2017

J Biomedical Materials Res

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Implant failures are primarily related to bacterial infections and inflammation. Nanocoating of implant devices with organic molecules is a method used for improving their integration into host tissues and limiting inflammation. Bioengineered plant-derived rhamnogalacturonan-Is (RG-Is) from pectins improve tissue regeneration and exhibit anti-inflammatory properties. Therefore, the aim of this study is to evaluate the in vitro effect of RG-I nanocoating on human gingival primary fibroblast (HGF) activity and proinflammatory response following Porphyromonas gingivalis (P. gingivalis) infection. Infected HGFs were incubated on tissue culture polystyrene (TCPS) plates coated with unmodified RG-I isolated from potato pectin (PU) and dearabinanated RG-I (PA). HGF morphology, proliferation, metabolic activity, and expression of genes responsible for extracellular matrix (ECM) turnover and proinflammatory response were examined. Following the P. gingivalis infection, PU and PA significantly promoted HGF proliferation and metabolic activity. Moreover, gene expression levels of IL1B, IL8, TNFA, and MMP2 decreased in the infected cells cultured on PU and PA, whereas the expression of COL1A1, FN1, and FGFR1 was upregulated. The results indicate that RG-Is are promising candidates for nanocoating of an implant surface, can reduce inflammation, and enhance implant integration, particularly in medically compromised patients with chronic inflammatory diseases such as periodontitis and rheumatoid arthritis. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3475-3481, 2017.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

Mieszkowska

Folkert

Gaber

et al. 2017

J Biomedical Materials Res

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Surface Nanocoating with Plant‐Derived Pectins Improves Fibroblast Response In Vitro

Folkert¹,

Mieszkowska²,

Gaber³

et al. 2018

Starch Stärke

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Pectins, plant‐derived polysaccharides, are novel candidates for biomaterial nanocoatings. Their chemical structure imitates the polysaccharides from the extracellular matrix of mammals, providing bio‐specific cell adhesion. It is possible to control the structure of pectin rhamnogalacturonan‐I (RG‐I) by enzymatic modification. RG‐Is has been proposed for surface nanocoating of orthopedic and dental titanium implants due to its effect on osseointegration with stimulation of bone and connective tissue healing. The aim of the current study is to evaluate in vitro, the impact of plant‐derived pectin RG‐I nanocoating on the cellular response of human fibroblasts. In this study, unmodified RG‐I and enzymatically modified RG‐I with low amount of arabinose and high amount of galactose are examined. All in vitro studies are performed on tissue culture polystyrene plates, whose surfaces are coated with unmodified and modified RG‐I. The results show that the nanocoating with pectin RG‐Is increas proliferation, cell metabolic activity, and gene expression of COL1A1, FN1, FGFR1, BMP7, and MMP2 as compared to control surface, without pectin coatings. The results show that nanocoating with RG‐Is has the capacity to improve fibroblast proliferation, differentiation, and extracellular matrix protein production, and may therefore be considered as a potential candidate for further improvement of bone and connective tissue regeneration.

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Effects of different extraction solvents on the compositions, primary structures, and anti-inflammatory activity of pectin from sweet potato processing by-products

Zhang,

Sun,

et al. 2025

Carbohydrate Polymers

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Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following <em>Porphyromonas gingivalis</em> infection

Cited by 12 publications

References 44 publications

Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

Surface Nanocoating with Plant‐Derived Pectins Improves Fibroblast Response In Vitro

Effects of different extraction solvents on the compositions, primary structures, and anti-inflammatory activity of pectin from sweet potato processing by-products

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