Plant-derived single domain COVID-19 antibodies

Shen, Andrew M; Malekshah, Obeid M.; Pogrebnyak, Natalia; Minko, Tamara

doi:10.1016/j.jconrel.2023.05.030

Cited by 3 publications

(3 citation statements)

References 52 publications

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“…To overcome this toxicity, plant-derived edible nanoparticles could be considered. Plants have the potential to be produced on a large scale, making them suitable for drug delivery applications [209].…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

Targeted Nanoparticle-Based Diagnostic and Treatment Options for Pancreatic Cancer

Gu,

Minko

2024

Cancers

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Pancreatic ductal adenocarcinoma (PDAC), one of the deadliest cancers, presents significant challenges in diagnosis and treatment due to its aggressive, metastatic nature and lack of early detection methods. A key obstacle in PDAC treatment is the highly complex tumor environment characterized by dense stroma surrounding the tumor, which hinders effective drug delivery. Nanotechnology can offer innovative solutions to these challenges, particularly in creating novel drug delivery systems for existing anticancer drugs for PDAC, such as gemcitabine and paclitaxel. By using customization methods such as incorporating conjugated targeting ligands, tumor-penetrating peptides, and therapeutic nucleic acids, these nanoparticle-based systems enhance drug solubility, extend circulation time, improve tumor targeting, and control drug release, thereby minimizing side effects and toxicity in healthy tissues. Moreover, nanoparticles have also shown potential in precise diagnostic methods for PDAC. This literature review will delve into targeted mechanisms, pathways, and approaches in treating pancreatic cancer. Additional emphasis is placed on the study of nanoparticle-based delivery systems, with a brief mention of those in clinical trials. Overall, the overview illustrates the significant advances in nanomedicine, underscoring its role in transcending the constraints of conventional PDAC therapies and diagnostics.

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Section: Metal Nanoparticlesmentioning

confidence: 99%

Targeted Nanoparticle-Based Diagnostic and Treatment Options for Pancreatic Cancer

Gu,

Minko

2024

Cancers

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“…Following the identification of their maximal expression levels in the top leaves, both the fusion proteins were purified from the infiltrated leaves using protein A affinity chromatography. Shen et al 2023 selected a SARS-CoV-2 spike protein-targeted human heavy chain variable domain (VHH) antibody fragment for rapid expression using plant cell suspensions and transgenic tobacco plants. Purification of this antibody was enabled through 6x His tag and C-Myc tag.…”

Section: Examples Of Plant-made Vaccine Purification Using Affinity Tagsmentioning

confidence: 99%

Recent advances in expression and purification strategies for plant made vaccines

Venkataraman,

Khan,

Habibi

et al. 2023

Front. Plant Sci.

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Plants have been explored as a platform to produce pharmaceutical proteins for over 20 years. Important features such as the cost-effectiveness of production, the ease of scaling up to manufacturing capacity, the lack of cold chain requirements and the ability to produce complex therapeutic proteins which are biologically and functionally identical to their mammalian counterparts, make plants a strong alternative for vaccine production. This review article focuses on both the expression as well as the downstream purification processes for plant made vaccines. Expression strategies including transgenic, transient and cell suspension cultures are outlined, and various plant tissues targeted such as leaves and seeds are described. The principal components used for downstream processing of plant made vaccines are examined. The review concludes with a reflection of the future benefits of plant production platforms for vaccine production.

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“…Plant-based recombinant COVID-19 works have mostly been based on transient expression in Nicotiana benthamiana (see Table 1 and references therein). Only few studies involved the stable transformation of plant cell suspensions ( Daniell et al., 2022 ; Rebelo et al., 2022 ; Ferreira et al., 2023 ; Shen et al., 2023 ). Among the proteins reported, there are structural proteins such as the spike (S) protein or S subunits (S1 and S2), the receptor-binding domain (RBD), the nucleocapsid (NC), envelope (E), and membrane (M) viral proteins, and others such as angiotensin-converting enzyme 2 (ACE2) receptor and antibodies against domains of the spike protein (RBD and S1) and the nucleocapsid protein ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platforms

Santoni,

Gutierrez-Valdes,

Pivotto

et al. 2024

Front. Plant Sci.

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The COVID-19 pandemic has underscored the need for rapid and cost-effective diagnostic tools. Serological tests, particularly those measuring antibodies targeting the receptor-binding domain (RBD) of the virus, play a pivotal role in tracking infection dynamics and vaccine effectiveness. In this study, we aimed to develop a simple enzyme-linked immunosorbent assay (ELISA) for measuring RBD-specific antibodies, comparing two plant-based platforms for diagnostic reagent production. We chose to retain RBD in the endoplasmic reticulum (ER) to prevent potential immunoreactivity issues associated with plant-specific glycans. We produced ER-retained RBD in two plant systems: a stable transformation of BY-2 plant cell culture (BY2-RBD) and a transient transformation in Nicotiana benthamiana using the MagnICON system (NB-RBD). Both systems demonstrated their suitability, with varying yields and production timelines. The plant-made proteins revealed unexpected differences in N-glycan profiles, with BY2-RBD displaying oligo-mannosidic N-glycans and NB-RBD exhibiting a more complex glycan profile. This difference may be attributed to higher recombinant protein synthesis in the N. benthamiana system, potentially overloading the ER retention signal, causing some proteins to traffic to the Golgi apparatus. When used as diagnostic reagents in ELISA, BY2-RBD outperformed NB-RBD in terms of sensitivity, specificity, and correlation with a commercial kit. This discrepancy may be due to the distinct glycan profiles, as complex glycans on NB-RBD may impact immunoreactivity. In conclusion, our study highlights the potential of plant-based systems for rapid diagnostic reagent production during emergencies. However, transient expression systems, while offering shorter timelines, introduce higher heterogeneity in recombinant protein forms, necessitating careful consideration in serological test development.

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Plant-derived single domain COVID-19 antibodies

Cited by 3 publications

References 52 publications

Targeted Nanoparticle-Based Diagnostic and Treatment Options for Pancreatic Cancer

Targeted Nanoparticle-Based Diagnostic and Treatment Options for Pancreatic Cancer

Recent advances in expression and purification strategies for plant made vaccines

Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platforms

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