Plant essential oils against bacteria isolated from fish: an in vitro screening and in vivo efficacy of Lippia origanoides

Bandeira, Guerino; Souza, Carine de Freitas; Baldissera, Matheus D.; Descovi, Sharine N.; Silveira, Bibiana Petri da; Tasca, Caiane; Mourão, Rosa Helena Veras; Vargas, Águeda Castagna de; Baldisserotto, Bernardo

doi:10.1590/0103-8478cr20190064

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…Similarly, previous studies have verified the ability of the EO of L . alba (De Souza et al, 2017), EOCC (Millezi et al, 2013), citral and carvacrol (Bandeira Junior et al, 2019) to prevent the formation of biofilms of Aeromonas spp.…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial and antibiofilm activities and synergism with florfenicol from the essential oils of Lippia sidoides and Cymbopogon citratus against Aeromonas hydrophila

Silva

Pires

Costa

et al. 2022

Journal of Applied Microbiology

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Aims Aeromonas hydrophila is an opportunistic bacterium, with a high capacity for biofilm production, which can cause severe damage in aquaculture. The objective of this study was to identify the chemical compounds of the essential oils of Lippia sidoides (EOLS) and Cymbopogon citratus (EOCC), and to evaluate the biocidal, antibiofilm and synergistic action with the antimicrobial florfenicol of these essential oils (EOs) against A. hydrophila. Methods and Results The antibacterial activity of EOLS and EOCC was verified by the minimum bactericidal concentration and by the action of these EOs against both forming and consolidated biofilms. The synergistic activity of EOs with florfenicol was performed using the checkerboard technique. The main component of EOLS and EOCC was carvacrol (44.50%) and α‐citral (73.56%), respectively. Both EOs showed weak inhibitory activity (≥3125.00 µg ml−1). Two bacterial isolates were able to produce biofilm, and EOLS and EOCC acted upon the bacterial isolates to prevent biofilm formation. A bactericidal effect was verified for EOLS in the previously consolidated biofilm for both isolates and for EOCC in only one of the isolates. In general, EOLS had a synergistic effect with florfenicol, while EOCF had an additive effect. Conclusions Both EOs were able to interfere with biofilm formation and did not have an antagonistic effect in combination with florfenicol. The best results were found for EOLS, which showed a synergistic effect with florfenicol and the ability to interfere in the formation of consolidated biofilm. Significance and Impact of the Study This study highlights the potential of EOLS and EOCC to interfere in biofilm and act in synergy with florfenicol to reduce the occurrence of A. hydrophila. Development of these compounds may contribute to the development of herbal medicines in aquaculture.

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Section: Discussionmentioning

confidence: 99%

“…An additive effect can reduce the time of antibacterial action compared to antimicrobials used alone (Scandorieiro et al, 2016). Bandeira Junior et al (2019) found that citral and carvacrol had an additive effect with florfenicol against A . hydrophila .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and antibiofilm activities and synergism with florfenicol from the essential oils of Lippia sidoides and Cymbopogon citratus against Aeromonas hydrophila

Silva

Pires

Costa

et al. 2022

Journal of Applied Microbiology

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“…A strong inhibitory effect of Ocimum basilicum EO with 3 µL/mL and 9 µL/mL MIC values was reported on A. hydrophila and A. veronii, respectively [105]. Among nine different herb EOs, Conobea scoparioides and Lippia origanoides EOs had remarkable activity against A. hydrophila with the low respective MIC and MBC values of 200 µg/mL [106].…”

Section: Oligohymenophorea Ichthyophthirius Multifiliismentioning

confidence: 97%

Antiparasitic and Antibacterial Functionality of Essential Oils: An Alternative Approach for Sustainable Aquaculture

Dawood

Basuini

Zaineldin³

et al. 2021

Pathogens

143

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Using synthetic antibiotics/chemicals for infectious bacterial pathogens and parasitic disease control causes beneficial microbial killing, produces multi-drug resistant pathogens, and residual antibiotic impacts in humans are the major threats to aquaculture sustainability. Applications of herbal products to combat microbial and parasitic diseases are considered as alternative approaches for sustainable aquaculture. Essential oils (EOs) are the secondary metabolites of medicinal plants that possess bioactive compounds like terpens, terpenoids, phenylpropenes, and isothiocyanates with synergistic relationship among these compounds. The hydrophobic compounds of EOs can penetrate the bacterial and parasitic cells and cause cell deformities and organelles dysfunctions. Dietary supplementation of EOs also modulate growth, immunity, and infectious disease resistance in aquatic organisms. Published research reports also demonstrated EOs effectiveness against Ichthyophthirius multifiliis, Gyrodactylus sp., Euclinostomum heterostomum, and other parasites both in vivo and in vitro. Moreover, different infectious fish pathogenic bacteria like Aeromonas salmonicida, Vibrio harveyi, and Streptococcus agalactiae destruction was confirmed by plant originated EOs. However, no research was conducted to confirm the mechanism of action or pathway identification of EOs to combat aquatic parasites and disease-causing microbes. This review aims to explore the effectiveness of EOs against fish parasites and pathogenic bacteria as an environment-friendly phytotherapeutic in the aquaculture industry. Moreover, research gaps and future approaches to use EOs for sustainable aquaculture practice are also postulated.

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“…Pirog et al (2019) found that essential oils combined with other antimicrobials could destroy yeast and bacterial biofilm, thereby significantly decreasing their MIC [61]. Bandeira Junior et al (2019) also found that carvacrol had an additive effect with florfenicol on A. drophila [62]. In this investigation, the antibacterial activity of carvacrol combined with neomycin sulfate showed an additive effect, suggesting that a combination of conventional antibiotics with carvacrol is a promising alternative for the control of A. hydrophila infection in aquaculture.…”

Section: Discussionmentioning

confidence: 52%

Antimicrobial and Antivirulence Activities of Carvacrol against Pathogenic Aeromonas hydrophila

et al. 2022

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Aeromonas hydrophila is a ubiquitous Gram-negative opportunistic pathogen in the freshwater environment and the most common cause of bacterial septicemia in aquaculture. In this study, we investigated the impact of carvacrol, a natural monoterpenoid found in herbs, on the virulence of A. hydrophila in vitro and the antibacterial effect in combination with antibiotics. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of carvacrol against A. hydrophila NJ-35 were 125 µg/mL and 250 µg/mL, respectively. Carvacrol could inhibit the virulence factors (biofilm, protease, exopolysaccharide, and hemolysin) of A. hydrophila, and the antibiofilm potential of carvacrol was further verified by microscopic detection. Transcriptional analyses showed that the gene expression of flaB, ompA, aha, ahp, ela, act, aerA, AhyR, and hly were marked as downregulated. The checkerboard assay results showed that carvacrol did not have an antagonistic effect in combination with antibiotics (florfenicol, enrofloxacin, thiamphenicol, or doxycycline hydrochloride) commonly used in aquaculture but possessed an additive-synergistic effect with neomycin sulfate. In vivo studies demonstrated that carvacrol protected grass carp (Ctenopharyngodon idella) from A. hydrophila infection. Our results indicated that carvacrol possessed significant anti-bacterial and anti-virulence effects on A. hydrophila.

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Plant essential oils against bacteria isolated from fish: an in vitro screening and in vivo efficacy of Lippia origanoides

Cited by 11 publications

References 27 publications

Antibacterial and antibiofilm activities and synergism with florfenicol from the essential oils of Lippia sidoides and Cymbopogon citratus against Aeromonas hydrophila

Antibacterial and antibiofilm activities and synergism with florfenicol from the essential oils of Lippia sidoides and Cymbopogon citratus against Aeromonas hydrophila

Antiparasitic and Antibacterial Functionality of Essential Oils: An Alternative Approach for Sustainable Aquaculture

Antimicrobial and Antivirulence Activities of Carvacrol against Pathogenic Aeromonas hydrophila

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