Plant essential oils against <i>Aeromonas hydrophila</i>
:<i> in vitro</i>
 activity and their use in experimentally infected fish

Sutili, Fernando Jonas; Silva, L. de Lima; Gressler, Letícia Trevisan; Battisti, Eduardo Kelm; Heinzmann, Berta Maria; Vargas, Agueda Castagna de; Baldisserotto, Bernardo

doi:10.1111/jam.12812

Cited by 47 publications

(49 citation statements)

References 32 publications

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“…); however, in some EOs, the amount of the main compound can reach up to more than 90% (Sutili et al . ). In plants, these molecules play a major role and act as antibacterials, antivirals, antifungals, insecticides and protect the plants from herbivores (Perricone et al .…”

Section: Essential Oilsmentioning

confidence: 97%

Plant essential oils as fish diet additives: benefits on fish health and stability in feed

Sutili

Gatlin

Heinzmann

et al. 2017

Reviews in Aquaculture

153

100

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Nutritional strategies focused on the use of phytochemicals as modulators of immunological and physiological responses, as well as antistress and antioxidant therapies and health promoters of the gastrointestinal tract have attracted increased interest in animal production. Over the past two decades, several studies have reported positive results of using plant essential oils (EOs) as dietary additive in several farm animal species. Recently, these nutritional alternatives have been evaluated and reported in fish production in order to enhance the resistance to diseases and prevent outbreaks, as well as to improve fish growth, animal welfare and feed utilization. However, despite the diverse and relevant biological activities reported, these phytochemicals are composed of often unstable and volatile molecules, which makes the chemical and physical characteristics of the EOs important aspects to be considered in fish nutrition research. This review summarizes the current knowledge regarding the use of plant EOs as fish dietary additives, focusing on their potential as modulators of the gut bacterial community and discussing some aspects regarding their stability in feeds.

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Section: Essential Oilsmentioning

confidence: 97%

Plant essential oils as fish diet additives: benefits on fish health and stability in feed

Sutili

Gatlin

Heinzmann

et al. 2017

Reviews in Aquaculture

153

100

View full text Add to dashboard Cite

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“…In a study conducted by Sutili et al . (), exposure to 20 and 40 mg l −1 EO of H. ringens showed relative survival rates of 70 and 66% through therapeutic baths of 1 h during 5 days. The result obtained for HEHR indicates a better relative survival rate (93·33%).…”

Section: Discussionmentioning

confidence: 88%

“…The EO of the species Ocimum americanum (20 mg l −1 ) promoted 75% relative survival rate (Sutili et al . ). On the other hand, the EO of Lippia alba (Verbenaceae) promoted a relative survival rate greater than 80% at 16 mg l −1 and greater than 90% at 40 mg l −1 (Sutili et al .…”

Section: Discussionmentioning

confidence: 97%

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Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria

et al. 2019

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Aims This study investigated the in vitro antibacterial activity of Hesperozygis ringens (Benth.) Epling leaf extracts against fish pathogenic bacteria, as well as the in vivo activity of the most active extract in silver catfish (Rhamdia quelen) experimentally infected with Aeromonas hydrophila. Moreover, the chemical composition of the extract used in the survival assay was evaluated. Methods and Results Only hexane extract (HEHR) showed in vitro antibacterial activity (MIC and MBC ranging from 1600 to 3200 μg ml−1) against clinical isolates of A. hydrophila, Raoultella ornithinolytica and Citrobacter freundii, obtained from naturally infected silver catfish, and A. hydrophilaATCC 7966. The major compound of the volatile fraction of HEHR was determined as pulegone. HEHR promoted a 93·33% relative survival rate of silver catfish experimentally infected with A. hydrophila 7 days after a single therapeutic bath at 30 mg l−1, while florfenicol at 4 mg l−1, which promoted a 60% relative survival rate. Conclusions The antibacterial activity of H. ringens (Benth.) Epling leaf extracts seems to be related to phytochemicals of apolar character, since HEHR promoted better survival rate of infected animals than florfenicol. Significance and Impact of the Study The HEHR has potential to be used in the control and treatment of bacterial infections in organic aquaculture.

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“…Preventive baths (1 h) with eugenol (5 and 10 mg/l) for 5 days did not alter phagocytic index, respiratory burst and myeloperoxidase activity and HACS in silver catfish ( Rhamdia quelen ) (Sutili et al., ). Silver catfish exposed to Hesperozygis ringens and O. americanum EOs (20 mg/l) diluted in ethanol (baths‐1 h daily/5 days) increased the HACS and cortisol levels in plasma (Sutili et al., ). Dietary supplementation of 3 g/kg of carvacrol in commercial diets improved lysozyme and myeloperoxidase activity in rainbow trout (Yilmaz et al., ).…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of plant essential oils on non‐specific immune parameters of red drum, Sciaenops ocellatus L.

Sutili

Gatlin

Rossi

et al. 2016

Animal Physiology Nutrition

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Phytochemicals such as plant essential oils (EOs) have been reported to favour various activities in the innate immune system of fish. Thus, the aim of this study was to verify the in vitro effect of three different plant EOs (Ocimum americanum, Cymbopogon flexuosus and Melaleuca alternifolia) on non-specific immune parameters and erythrocyte osmotic fragility of red drum, Sciaenops ocellatus. Concentrations of each plant EO evaluated in preparations of head-kidney macrophages, blood leucocytes and blood plasma were as follows: 0.0 (control), 1.0, 2.0, 4.0, 8.0, and 16.0 μg/ml. Red drum head-kidney macrophages significantly increased extracellular superoxide anion production when exposed (20 h) to O. americanum EO (1.0-8.0 μg/ml) and C. flexuosus EO (2.0 and 4.0 μg/ml). The respiratory burst of blood leucocytes (NBT test) significantly increased in all concentrations when compared to the respective control group, for all EOs. At the highest concentration (16.0 μg/ml), C. flexuosus EO significantly inhibited the haemolytic activity of complement system in red drum blood after 1 h exposure. None of the tested concentrations significantly altered plasma lysozyme activity or erythrocyte osmotic fragility after exposing (1 h) red drum whole blood to each EO. This study demonstrated that these plant EOs are capable of triggering superoxide anion production in red drum leucocytes (head-kidney macrophages and/or blood leucocytes). In vivo studies are warranted to address their potential as immunostimulants in the diet of red drum and other aquacultured species.

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Plant essential oils against Aeromonas hydrophila : in vitro activity and their use in experimentally infected fish

Abstract: These products can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, with antimicrobial and/or immunostimulant properties.

Cited by 47 publications

References 32 publications

Plant essential oils as fish diet additives: benefits on fish health and stability in feed

Plant essential oils as fish diet additives: benefits on fish health and stability in feed

Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria

In vitro effects of plant essential oils on non‐specific immune parameters of red drum, Sciaenops ocellatus L.

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