Plant extracts as modulators of genotoxic effects

Sarkar, Debasis; Sharma, Archana; Talukder, Geeta

doi:10.1007/bf02856614

Cited by 34 publications

(19 citation statements)

References 98 publications

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“…Essentially, natural dyes are biodegradable in nature (Daniel et al 2006). Certain intrinsic components of the extracts could act as nonspecific redox agents, free radical scavengers, or ligands for binding metals or toxic principles, which could possibly explain the record of the least mitotic abnormalities in annatto extract treated root tips (Sarkar et al 1996). In the previous report (Aranez and Rubio 1996) on annatto genotoxicity analysis, chloroform extract and petroleum ether extract showed low levels of mitotic index compared to the control.…”

Section: Discussionmentioning

confidence: 92%

Cytotoxic Evaluation of Annatto (Bixa orellana L.) Dye Compared with Orange Red

Joseph

Siril

2010

CYTOLOGIA

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Summary Cytotoxic evaluation of annatto (Bixa orellana L.) seed pulp extract on Allium cepa root tip cells was carried out. The annatto dye was extracted using different solvents viz. alkaline water, ethyl alcohol and chloroform : alcohol mixture (1 : 3). Crude extract evaporated and dry residual powder recovered. The powder was dissolved in water at different concentrations (0.1%, 0.5% and 1%) and was used to treat onion root tips for 12 h and the mitotic behaviour was recorded. The results were compared with a synthetic dye orange red at 2 concentrations (0.05 and 0.1%). Annatto extracts treated cells showed normal mitotic behaviour whereas orange red (0.1% w/v) showed complete arrest of cell division. The synthetic dye at low concentration (0.05% w/v) showed diverse forms of mitotic abnormalities like single or double nuclear lesions, single, double or multiple bridges, metaphase clumping, tropokinensis and chromosome fragments. Cyototoxicity test system based on A. cepa root tips has proved that annatto dye is significantly less toxic compared to its synthetic alternative, orange red.

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Section: Discussionmentioning

confidence: 92%

Cytotoxic Evaluation of Annatto (Bixa orellana L.) Dye Compared with Orange Red

Joseph

Siril

2010

CYTOLOGIA

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“…A considerable body of epidemiological evidence indicates that diets high in fruit and vegetables are inversely related to risk of chronic, degenerative diseases such as coronary heart disease and certain cancers. Much research effort has focused on the identification of phytochemicals in fruit and vegetables that exert beneficial effects and elucidation of the mechanisms by which they inhibit cellular injury and degeneration (Sarkar et al, 2008).…”

Section: Plant Extracts As Genotoxic Agentsmentioning

confidence: 99%

Potential Genotoxic and Cytotoxic Effects of Plant Extracts

Çelik¹

2012

A Compendium of Essays on Alternative Therapy

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“…It is evident from data that out of total chemical structures discovered, a major share was directly derived from higher plants (Kinghorn andBalandrin 1993, Sarkar andSharma 1996) and several of them can be used as antitumour agents (Mukherjee et al 2001).…”

mentioning

confidence: 99%

Evaluation of anti-genotoxicity of the leaf extracts of Morinda citrifolia Linn.

Sreeranjini¹,

Siril²

2011

Plant Soil Environ.

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Morinda citrifolia Linn. (family Rubiaceae) is a small tree occurring in tropical areas of the world. The plant contains several medicinally active components that exhibit the therapeutic effects such as antibacterial, antiviral and anticancer activities. Anti-genotoxic effects of aqueous extracts prepared using dried leaves of M. citrifolia was studied. Allium cepa root tip meristem cells treated with 7% hydrogen peroxide were used for eliciting antigenotoxicity. For this purpose experiments were performed with A. cepa onion bulbs treated for 24 h with different concentrations (15 or 30 g/L) of aqueous extract with or without pre-treatment (1 h) with 7% hydrogen peroxide. A significant reduction in mitotic index was recorded in treatment groups over negative control. Chromosomal aberrations such as breaks, bridges, stickiness and polar deviations were observed in positive control and treatment groups. The highest (21.48) percentage of chromosomal aberrations was noticed in positive control. A significant reduction in chromosomal aberrations (9.39) was recorded in root tips treated with hydrogen peroxide followed by 15 g/L extract. H 2 O 2 induced chromosomal aberrations were reduced due to leaf extract treatment indicates antimutagenic potential of the M. citrifolia. The observations suggest that M. citrifolia aqueous leaf extracts have antimitotic and anti-genotoxic effects; consequently oxidative stress induced aberrations due to H 2 O 2 are efficiently restored in the extract treated A. cepa root meristem cells.

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Plant extracts as modulators of genotoxic effects

Cited by 34 publications

References 98 publications

Cytotoxic Evaluation of Annatto (Bixa orellana L.) Dye Compared with Orange Red

Cytotoxic Evaluation of Annatto (Bixa orellana L.) Dye Compared with Orange Red

Potential Genotoxic and Cytotoxic Effects of Plant Extracts

Evaluation of anti-genotoxicity of the leaf extracts of Morinda citrifolia Linn.

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