Plant-feeding insects harbor double-stranded RNA viruses encoding a novel proline-alanine rich protein and a polymerase distantly related to that of fungal viruses

Spear, Allyn; Sisterson, Mark S.; Yokomi, R. K.; Stenger, Drake C.

doi:10.1016/j.virol.2010.05.015

Cited by 43 publications

(47 citation statements)

References 30 publications

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“…However, BpRV1 with two dsRNA segments differs from these three viruses in the number of dsRNA segments since genomes of SFV1, CiTV1, and UmVH1 are monosegmented (26,46). Although BpRV1 and RnMBV1 have bipartite dsRNA genomes and their RdRps share sequence similarities, BpRV1 differs from RnMBV1 in the expression strategy for the generation of RdRp and the structural proteins.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Spear et al (46) proposed that the proline-alaninerich protein (PArp) encoded by SFV1 and CiTV1 may act as a scaffold protein to interact with some membrane components in insect cells, since many animal viruses can utilize a protein scaffold containing proline-rich "late" domain motifs to hijack host proteins involved in the vacuolar protein sorting pathway, resulting in the formation of multivesicular bodies (8). In the present study, the formation of abundant vacuole-like membranous structures and small membranous vesicles in hyphal cells infected with BpRV1 was observed (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a Novel Bipartite Double-Stranded RNA Mycovirus Conferring Hypovirulence in the Phytopathogenic Fungus Botrytis porri

Jin

Zhang

et al. 2012

J Virol

116

140

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The ascomycete Botrytis porri causes clove rot and leaf blight of garlic worldwide. We report here the biological and molecular features of a novel bipartite double-stranded RNA (dsRNA) mycovirus named B otrytis p orri R NA v irus 1 (BpRV1) from the hypovirulent strain GarlicBc-72 of B. porri . The BpRV1 genome comprises two dsRNAs, dsRNA-1 (6,215 bp) and dsRNA-2 (5,879 bp), which share sequence identities of 62 and 95% at the 3′- and 5′-terminal regions, respectively. Two open reading frames (ORFs), ORF I (dsRNA-1) and ORF II (dsRNA-2), were detected. The protein encoded by the 3′-proximal coding region of ORF I shows sequence identities of 19 to 23% with RNA-dependent RNA polymerases encoded by viruses in the families Totiviridae , Chrysoviridae , and Megabirnaviridae . However, the proteins encoded by the 5′-proximal coding region of ORF I and by the entire ORF II lack sequence similarities to any reported virus proteins. Phylogenetic analysis showed that BpRV1 belongs to a separate clade distinct from those of other known RNA mycoviruses. Purified virions of ∼35 nm in diameter encompass dsRNA-1 and dsRNA-2, and three structural proteins (SPs) of 70, 80, and 85 kDa, respectively. Peptide mass fingerprinting analysis revealed that the 80- and 85-kDa SPs are encoded by ORF I, while the 70-kDa SP is encoded by ORF II. Introducing BpRV1 purified virions into the virulent strain GarlicBc-38 of B. porri caused derivative 38T reduced mycelial growth and hypovirulence. These combined results suggest that BpRV1 is a novel bipartite dsRNA virus that possibly belongs to a new virus family.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Bipartite Double-Stranded RNA Mycovirus Conferring Hypovirulence in the Phytopathogenic Fungus Botrytis porri

Jin

Zhang

et al. 2012

J Virol

116

140

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“…Two of these conserved sequences (WSASGG and GKNRVIWNT) represent conserved domains of RdRp from dsRNA mycoviruses (Bruenn, 1993; Roouthier & Bruenn, 1998;Spear et al, 2010 …”

Section: Introductionmentioning

confidence: 99%

“…Two of these conserved sequences (WSASGG and GKNRVIWNT) represent conserved domains of RdRp from dsRNA mycoviruses (Bruenn, 1993;Roouthier & Bruenn, 1998;Spear et al, 2010 Asterisks, colons and dots indicate identical amino acid residues, conserved amino acid residues and semiconserved amino acid residues, respectively. FIGURE 2 -Phylogenetic tree based on the deduced amino acid sequence of the putative RdRp from 32 isolates of papaya meleira virus (PMeV) from Brazil and a member of the family Totiviridae, Phebiopsis gigantea virus 2 (PgV-2, AM111097.2).…”

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confidence: 99%

Genetic diversity studies of Papaya meleira virus

Daltro¹,

Abreu²,

Aragão³

et al. 2014

Trop. plant pathol.

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Papaya (Carica papaya) is a fruit crop of great economic and social importance for Brazil and other papaya-producing countries. Brazil is the second largest producer in the world. The papaya sticky disease, caused by Papaya meleira virus (PMeV), has caused great losses in the major Brazilian papaya-producing states. In order to estimate the genetic diversity of PMeV, latex samples were collected from papaya plants in the states of Bahia, Espírito Santo, Pernambuco, Ceará and Rio Grande do Norte, and total RNA was extracted. Specific primer for the replicase region allowed the amplification, by RT-PCR, of a fragment of approximately 560 bp from 31 isolates. The sequence analysis indicated a level of conservation greater than 88% among isolates. Furthermore, comparative analyzes indicated that PMeV has similarity with mycoviruses of the family Totiviridae. This phylogenetic relationship was reinforced by the presence of conserved motifs within in the RdRp regions from mycoviruses. Key words: Carica papaya, Totiviridae, sticky disease, variability, viruses.Papaya (Carica papaya L.), is a fruit crop that originated and was domesticated in southern Mexico, and now is globally distributed and cultivated in many countries around the world. According to data from the Food and Agriculture Organization (FAO), the global production of papaya in 2010 was about 8 million tons, representing 10% of all tropical fruits produced worldwide (faostat.fao. org). The world's leading producers are India (42%) and Brazil (23.4%). Papaya production in Brazil was about 1,87 million tons on 35,000 hectares, with a value estimated at US$ 650 million (IBGE, 2011). The major papaya producing states are Bahia (928,000 tons), followed by Espírito Santo (560,000 tons), Ceará (112,000 tons) and Rio Grande do Norte (69,000 tons).The main factors that threaten papaya production are insect pests and diseases, especially those caused by viruses. The papaya sticky disease, caused by Papaya meleira virus (PMeV) was reported in the 1980's affecting papaya plants in the south of Bahia and north of Espírito Santo (Nakagawa et al., 1987;Rodrigues et al., 1989). Soon afterwards, the disease was detected in the states of Pernambuco, Ceara and Rio Grande do Norte (Barbosa et al., 1998a,b). Outside Brazil, Mexico is the only other country where the disease has been reported (Perez-Brito et al., 2012).The disease's typical symptoms are: excessive exudation of watery latex on the fruit surface, which oxidizes and gives the fruit a stained and sticky aspect; fruit malformation, uneven ripening and changes in the organolepctic properties, rendering it unmarketable.The PMeV genome composition and size are uncommon among plant viruses. PMeV has a double strand RNA (dsRNA) genome, approximately 12 Kb in length, packed into an isometric particle with ca. 50 nm diam (Kitajima et al., 1993; Maciel-Zambolin et al., 2003). The virus can be transmitted by latex injection into the stem apex, and previous work suggests that the virus could be transmitted by Bemisia ...

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“…Other examples are abundant and a few are provided: the closest relatives of mycoreoviruses are tickborne, mammal-infecting reoviruses such as Colorado tick fever virus (family Reoviridae, genus Coltivirus) ; the closest relatives of the oomycete-infecting virus Phytophthora infestans RNA virus 1 (PiRV-1) (Cai et al, 2009) are the mammalinfecting astroviruses; Botrytis virus F (family Gammaflexiviridae, genus Mycoflexivirus) and Botrytis virus X (family Alphaflexiviridae, genus Botrexvirus) are closely related to plant-infecting potexviruses (Howitt et al, 2001). Recent examples include viruses from two plant-feeding hemipterans, a leafhopper and a treehopper, that were related to the Totiviridae and Megabirnaviridae families of fungal and lower eukaryote viruses (Spear et al, 2010), and a new virus from S. sclerotiorum whose closest identified relative is Hepatitis E virus (family Hepeviridae, genus Hepevirus) (Liu et al, 2009). Finally, the closest relatives of fungal dsRNA viruses of the family Partitiviridae are plant cryptic viruses of the same family (Ghabrial et al, 2005).…”

Section: Relationships Of Fungal Viruses To Other Eukaryotic Virusesmentioning

confidence: 99%

The Ecology and Evolution of Fungal Viruses

Milgroom

Hillman

2011

Studies in Viral Ecology

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Plant-feeding insects harbor double-stranded RNA viruses encoding a novel proline-alanine rich protein and a polymerase distantly related to that of fungal viruses

Cited by 43 publications

References 30 publications

Characterization of a Novel Bipartite Double-Stranded RNA Mycovirus Conferring Hypovirulence in the Phytopathogenic Fungus Botrytis porri

Characterization of a Novel Bipartite Double-Stranded RNA Mycovirus Conferring Hypovirulence in the Phytopathogenic Fungus Botrytis porri

Genetic diversity studies of Papaya meleira virus

The Ecology and Evolution of Fungal Viruses

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