Plant flavonoids target <i><scp>P</scp>seudomonas syringae</i> pv. tomato <scp>DC</scp>3000 flagella and type <scp>III</scp> secretion system

Vargas, Paola; Farias, Gabriela A.; Nogales, Joaquina; Prada, Harold; Carvajal, Vivian; Barón, Matilde; Rivilla, Rafael; Martín, Marta; Olmedilla, Adela; Gallegos, Marı́a-Trinidad

doi:10.1111/1758-2229.12086

Cited by 73 publications

(84 citation statements)

References 58 publications

(89 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate the role of flagella in P. syringae pv. tomato DC3000 motility, we used an fleQ mutant deficient in the master regulator for flagellar synthesis (46) and an fliC mutant impaired in flagellin production (previously called flaA) (51). Surface (swarming and sliding) motility assays were carried out in PAG (0.5% agar) plates to detect the ability to spread over the agar surface.…”

Section: Resultsmentioning

confidence: 99%

“…P. syringae pv. tomato DC3000 produces one to five polar flagella (46,47), but little is known about their expression and regulation. This bacterium also produces the biosurfactant syringafactin, a peptide composed of eight amino acids with six structural variants that differ in a single amino acid and the length of the carbon chain attached (3-hydroxydecanoyl or 3-hydroxydodecanoyl).…”

mentioning

confidence: 99%

“…By using mutants fleQ and fliC, lacking flagella (46,51), and syfA, impaired in the production of syringafactin (48), we have observed that the fleQ mutation alters bacterial surface motility and biosurfactant production, uncovering a new type of motility for P. syringae pv. tomato DC3000 on semisolid surfaces.…”

mentioning

confidence: 99%

See 2 more Smart Citations

FleQ Coordinates Flagellum-Dependent and -Independent Motilities in Pseudomonas syringae pv. tomato DC3000

Nogales

Vargas

Farias

et al. 2015

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

bMotility plays an essential role in bacterial fitness and colonization in the plant environment, since it favors nutrient acquisition and avoidance of toxic substances, successful competition with other microorganisms, the ability to locate the preferred hosts, access to optimal sites within them, and dispersal in the environment during the course of transmission. In this work, we have observed that the mutation of the flagellar master regulatory gene, fleQ, alters bacterial surface motility and biosurfactant production, uncovering a new type of motility for Pseudomonas syringae pv. tomato DC3000 on semisolid surfaces. We present evidence that P. syringae pv. tomato DC3000 moves over semisolid surfaces by using at least two different types of motility, namely, swarming, which depends on the presence of flagella and syringafactin, a biosurfactant produced by this strain, and a flagellumindependent surface spreading or sliding, which also requires syringafactin. We also show that FleQ activates flagellum synthesis and negatively regulates syringafactin production in P. syringae pv. tomato DC3000. Finally, it was surprising to observe that mutants lacking flagella or syringafactin were as virulent as the wild type, and only the simultaneous loss of both flagella and syringafactin impairs the ability of P. syringae pv. tomato DC3000 to colonize tomato host plants and cause disease. Motility plays a pivotal role in the spreading of bacteria across surfaces and colonization, contributing to the formation of structured communities called biofilms (1). Efficient bacterial motility under diverse environmental conditions, from liquid to semisolid and solid surfaces, is achieved by flagellum-dependent swimming and swarming or flagellum-independent twitching, gliding, nonsocial gliding, and sliding (2, 3, 4). Swimming is a flagellum-driven motility observed in bacteria moving through liquids or semisolid media, such as low-percentage agar (0.2% to 0.4%). Twitching is a slow cell movement on surfaces mediated by the extension and retraction of type IV pili (5). Gliding, a surface movement extensively studied in myxobacteria, does not require flagella or pili but involves focal adhesion complexes, cell surfaceassociated complexes that anchor the bacterium to a substrate and might act as a motor (6). Sliding is a passive form of surface spreading by expansion that is powered by the outward pressure of bacterial growth and facilitated by compounds that reduce friction between cells and surfaces (3). Sliding or spreading by expansion has been observed in a diverse group of bacteria, such as mycobacteria, Bacillus subtilis, Vibrio cholerae, Serratia marcescens, Pseudomonas aeruginosa, or Legionella pneumophila (7-12), in which a strong correlation between sliding and production of surfactants has been established. Furthermore, sliding is easily mistaken for swarming motility and can occur when flagella are disrupted in bacteria that normally would swarm (7,8,13,14). Swarming is a rapid and coordinated movement of bacterial popul...

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

FleQ Coordinates Flagellum-Dependent and -Independent Motilities in Pseudomonas syringae pv. tomato DC3000

Nogales

Vargas

Farias

et al. 2015

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…phaseolicola is also puzzling, as are the sensory proteins perceiving the signals, the links between signal sensors and the possible cross talk between T3SSs and other regulated processes in phytopathogenic bacteria (43). Recently, structurally diverse secondary metabolites have been identified as modulators of T3SS expression and assembly in various phytopathogenic bacteria (62,114,120,131).…”

Section: Plant Inducibilitymentioning

confidence: 99%

A Career on Both Sides of the Atlantic: Memoirs of a Molecular Plant Pathologist

Panopoulos

2017

Annu. Rev. Phytopathol.

View full text Add to dashboard Cite

This article recounts the experiences that shaped my career as a molecular plant pathologist. It focuses primarily on technical and conceptual developments in molecular phytobacteriology, shares some personal highlights and untold stories that impacted my professional development, and describes the early years of agricultural biotechnology. Writing this article required reflection on events occurring over several decades that were punctuated by a mid-career relocation across the Atlantic. I hope it will still be useful, informative, and enjoyable to read.

show abstract

“…MCFI was used to test the response of tomato plants to Pto mutants in the multidrug resistance efflux pump MexAB-OprM. Images of F440 and F440/F690 could be used to classify them in terms of virulence [73].…”

Section: Mcfi To Monitor Plants Infected With Bacteriamentioning

confidence: 99%

Picturing pathogen infection in plants

Barón

Pineda

Pérez-Bueno

2016

Zeitschrift Für Naturforschung C

Self Cite

View full text Add to dashboard Cite

Several imaging techniques have provided valuable tools to evaluate the impact of biotic stress on host plants. The use of these techniques enables the study of plant-pathogen interactions by analysing the spatial and temporal heterogeneity of foliar metabolism during pathogenesis. In this work we review the use of imaging techniques based on chlorophyll fluorescence, multicolour fluorescence and thermography for the study of virus, bacteria and fungi-infected plants. These studies have revealed the impact of pathogen challenge on photosynthetic performance, secondary metabolism, as well as leaf transpiration as a promising tool for field and greenhouse management of diseases. Images of standard chlorophyll fluorescence (Chl-F) parameters obtained during Chl-F induction kinetics related to photochemical processes and those involved in energy dissipation, could be good stress indicators to monitor pathogenesis. Changes on UV-induced blue (F440) and green fluorescence (F520) measured by multicolour fluorescence imaging in pathogen-challenged plants seem to be related with the up-regulation of the plant secondary metabolism and with an increase in phenolic compounds involved in plant defence, such as scopoletin, chlorogenic or ferulic acids. Thermal imaging visualizes the leaf transpiration map during pathogenesis and emphasizes the key role of stomata on innate plant immunity. Using several imaging techniques in parallel could allow obtaining disease signatures for a specific pathogen. These techniques have also turned out to be very useful for presymptomatic pathogen detection, and powerful non-destructive tools for precision agriculture. Their applicability at lab-scale, in the field by remote sensing, and in high-throughput plant phenotyping, makes them particularly useful. Thermal sensors are widely used in crop fields to detect early changes in leaf transpiration induced by both air-borne and soil-borne pathogens. The limitations of measuring photosynthesis by Chl-F at the canopy level are being solved, while the use of multispectral fluorescence imaging is very challenging due to the type of light excitation that is used.Keywords: biotic stress; chlorophyll fluorescence imaging; multicolor fluorescence imaging; plant pathogens; thermography. Dedication: This work is dedicated to the memory of Professor Peter Böger. He will always bring to my heart (M. B.) the best memories of my postdoc at Lake Constance.

show abstract

Plant flavonoids target Pseudomonas syringae pv. tomato DC3000 flagella and type III secretion system

Cited by 73 publications

References 58 publications

FleQ Coordinates Flagellum-Dependent and -Independent Motilities in Pseudomonas syringae pv. tomato DC3000

FleQ Coordinates Flagellum-Dependent and -Independent Motilities in Pseudomonas syringae pv. tomato DC3000

A Career on Both Sides of the Atlantic: Memoirs of a Molecular Plant Pathologist

Picturing pathogen infection in plants

Contact Info

Product

Resources

About